The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
FKBP-rapamycin inhibits a cyclin-dependent kinase activity and a cyclin D1-Cdk association in early G1 of an osteosarcoma cell line
Open accessAbstract
Upon entering a cell the natural product rapamycin, like the structurally related immunosuppressant FK506, associates with members of the FKBP family of proteins. One or more of the resulting FKBP-rapamycin complexes blocks signaling pathways emanating from some growth factor receptors. Recently, the addition of rapamycin was shown to inhibit the phosphorylation and activation of a 70-kDa ribosomal S6 protein kinase, which normally occurs minutes after the activation of certain cytokine and growth factor receptors. We now report that rapamycin can be added 4 to 6 h after the addition of serum growth factors to quiescent human osteosarcoma cells and still arrest these cells in G1. This window of action correlates with the inducible appearance of a cyclin-dependent kinase (cdk) activity, and the induction of this activity is inhibited by the addition of rapamycin. Furthermore, p36cyclin D1 associates with this cdk protein complex in lysates of untreated cells, but does not associate with this cdk protein complex in lysates of rapamycin-treated cells. Together, these studies demonstrate that FKBP-rapamycin can modulate a cyclin-dependent kinase activity and a cyclin D1-cdk association during early G1 in MG-63 human osteosarcoma cells.
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“Anthracyclines are among the most widely used antitumor antibiotics in the clinic and exert antitumor activity mainly by inhibiting topoisomerase II [215,216,217,218,219].”
From Marine Natural Products: A Source of Novel Anticancer Drugs (2019) · cites this paper as [218]
Citations
Cites
Targets for Cell Cycle Arrest by the Immunosuppressant Rapamycin in Yeast
Heitman, J., Movva, N. R., & Hall, M. N. (1991) · Science · doi:10.1126/science.1715094 · Cited by 2,055
Chung, J., Kuo, C. J., Crabtree, G. R., & Blenis, J. (1992) · Cell · doi:10.1016/0092-8674(92)90643-q · Cited by 1,151
Formation and Activation of a Cyclin E-cdk2 Complex During the G 1 Phase of the Human Cell Cycle
Koff, A., Giordano, A., Desai, D., Yamashita, K., Harper, J. W., Elledge, S. J., Nishimoto, T., Morgan, D. O., Franza, B. R., & Roberts, J. M. (1992) · Science · doi:10.1126/science.1388288 · Cited by 1,043
Xiong, Y., Zhang, H., & Beach, D. (1992) · Cell · doi:10.1016/0092-8674(92)90518-h · Cited by 1,021
Functional interactions of the retinoblastoma protein with mammalian D-type cyclins
Ewen, M. E., Sluss, H. K., Sherr, C. J., Matsushime, H., Kato, J.-Y., & Livingston, D. M. (1993) · Cell · doi:10.1016/0092-8674(93)90136-e · Cited by 1,010
A family of human cdc2‐related protein kinases.
Meyerson, M. M., Enders, G. H., Wu, C. L., Su, L., Gorka, C., Nelson, C. E., Harlow, E., & Tsai, L. (1992) · The EMBO Journal · doi:10.1002/j.1460-2075.1992.tb05360.x · Cited by 925
Matsushime, H., Ewen, M. E., Strom, D. K., Kato, J.-Y., Hanks, S. K., Roussel, M. F., & Sherr, C. J. (1992) · Cell · doi:10.1016/0092-8674(92)90360-o · Cited by 897
Kunz, J., Henríquez, R., Schneider, U., Deuter‐Reinhard, M., Movva, N. R., & Hall, M. N. (1993) · Cell · doi:10.1016/0092-8674(93)90144-f · Cited by 859
Physical interaction of the retinoblastoma protein with human D cyclins
Dowdy, S. F., Hinds, P. W., Louie, K., Reed, S. I., Arnold, A., & Weinberg, R. A. (1993) · Cell · doi:10.1016/0092-8674(93)90137-f · Cited by 746
Bierer, B. E., Mattila, P. S., Standaert, R. F., Herzenberg, L. A., Burakoff, S. J., Crabtree, G. R., & Schreiber, S. L. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.23.9231 · Cited by 706
Self-citation
Research works printed but not linked: 5
- Koltin, 1991, Mol. Cell. Biol., 11, 1718
- Carbonaro-Hall, 1993, Oncogene, 8, 1649
- Hall, 1993, Oncogene, 8, 1377
- Williams, 1992, Oncogene, 423
- Sherr, 1992, Ciba Found. Symp., 170, 209
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Smyth, L. A., Matthews, T. P., & Collins, I. (2011) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2011.03.069 · Cited by 19
Bonatti, S., Simili, M., Galli, A., Bagnato, P., Pigullo, S., Schiestl, R. H., & Abbondandolo, A. (1998) · Chromosoma · doi:10.1007/s004120050335 · Cited by 19
Carreau, A., Gueugnon, J., Bénavidès, J., & Vigé, X. (1997) · Neuropharmacology · doi:10.1016/s0028-3908(97)00160-3 · Cited by 18
Hypersensitivity to rapamycin of BJAB B lymphoblastoid cells.
Kay, J. E., Smith, M. C., Frost, V. J., & Morgan, G. Y. (1996) · PubMed Central · Cited by 15
Targeted therapies for kidney cancer in urologic practice
Haas, N. B., & Uzzo, R. G. (2007) · Urologic Oncology Seminars and Original Investigations · doi:10.1016/j.urolonc.2007.05.009 · Cited by 14
Branched‐chain amino acid synthesis is coupled to TOR activation early in the cell cycle in yeast
Blank, H. M., Reuse, C., Schmidt‐Hohagen, K., Hammer, S. E., Hiller, K., & Polymenis, M. (2023) · EMBO Reports · doi:10.15252/embr.202357372 · Cited by 11
Emerging strategies for the clinical application of rapamycin
Kahan, B. D. (1998) · Clinical Biochemistry · doi:10.1016/s0009-9120(98)00044-7 · Cited by 12
Adjuvant therapy for renal cell carcinoma
Haas, N. B., & Uzzo, R. G. (2008) · Current Oncology Reports · doi:10.1007/s11912-008-0037-4 · Cited by 12
Rapamycin induces autophagy and apoptosis in Kaposiform hemangioendothelioma primary cells in vitro
Wang, Z., Han, Q., Wang, J., Yao, W., Wang, L., & Li, K. (2022) · Journal of Pediatric Surgery · doi:10.1016/j.jpedsurg.2022.02.032 · Cited by 11
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Lagercrantz, J., Larsson, C., Grimmond, S. M., Skogseid, B. M., Gobl, A., Friedman, E., Carson, E., Phelan, C., Öberg, K., Nordenskjöld, M., et al. (1995) · Journal of Internal Medicine · doi:10.1111/j.1365-2796.1995.tb00930.x · Cited by 11
Tyrosine Kinase Inhibitors and Anti-Angiogenic Therapies in Kidney Cancer
Haas, N. B., & Uzzo, R. G. (2007) · Current Treatment Options in Oncology · doi:10.1007/s11864-007-0031-3 · Cited by 10
Probing T-cell signal transduction pathways with the immunosuppressive drugs, FK-506 and rapamycin
Siekierka, J. J. (1994) · Immunologic Research · doi:10.1007/bf02918272 · Cited by 10
Younis, A., Riaz, A., Javaid, F., Ahsan, M. T., Tariq, U., Aslam, S., & Asif, M. (2015) · Time to knit · doi:10.51847/vulzsqgqhh · Cited by 9
Kawamata, T., Yamaguchi, T., Shin‐ya, K., & Hori, T. (2003) · Neurochemical Research · doi:10.1023/a:1024284529945 · Cited by 9
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Nelson, F. C., Stachel, S. J., & Mattes, J. F. (1994) · Tetrahedron Letters · doi:10.1016/s0040-4039(00)78342-0 · Cited by 8
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Amaya, T., Hiroi, J., & Lawrence, I. D. (2003) · Elsevier eBooks · doi:10.1016/b978-012526451-8/50010-2 · Cited by 8
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Xu, J., Shi, P., Zhao, X., Shen, P., Feng, Y., Lu, F., & Shi, D. (2020) · Reproduction in Domestic Animals · doi:10.1111/rda.13868 · Cited by 6
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Mascarell, L., Auger, R., Kanellopoulos, J., & Truffa‐Bachi, P. (2006) · Molecular Immunology · doi:10.1016/j.molimm.2005.10.019 · Cited by 4
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Brazelton, T. R., Cheung, A., & Morris, R. E. (1997) · Springer eBooks · doi:10.1007/978-3-642-60572-7_44 · Cited by 4
Sikpi, M. O. (2000) · International Journal of Radiation Biology · doi:10.1080/095530000138835 · Cited by 4
Evolving role of sirolimus immunosuppression after organ transplantation
Formica, R. N., Friedman, A. L., & Lorber, M. I. (2002) · Current Opinion in Organ Transplantation · doi:10.1097/00075200-200212000-00009 · Cited by 4
Revisªo/AtualizaÁªo em Transplante Renal: Novos agentes imunossupressores
Abbud‐Filho, M., & Ramalho, H. J. (1997) · Cited by 3
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Pacheco, J. E., Wills, R. C., & Hammond, G. R. V. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1142-5_7 · Cited by 3
Kim, L. (2008) · Cited by 2
The Role of Target of Rapamycin Signaling in Tuberous Sclerosis Complex
Manning, B. D. (2010) · Wiley eBooks · doi:10.1002/9783527630073.ch6 · Cited by 2
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Zhukova, Z. A. С. (2024) · Genes and Cells · doi:10.17816/gc630218 · Cited by 1
Pandey, A., & Tripathi, S. (2014) · International Research Journal of Pharmacy · doi:10.7897/2230-8407.050206 · Cited by 1
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Dorff, T. B., & Mack, P. C. (2015) · Springer eBooks · doi:10.1007/978-3-319-17903-2_18 · Cited by 1
Thomas, H. E., Veiga, S. R., Thomas, G., & Kozma, S. C. (2015) · Springer eBooks · doi:10.1007/978-2-8178-0492-7_2 · Cited by 1
The TGFβ receptors and signaling pathways
Datto, M., Bassing, C. H., & Wang, X. (1996) · Growth factors and cytokines in health and disease · doi:10.1016/s1874-5687(96)80017-6 · Cited by 1
Studies on the PI3K/mTOR pathway as cytostatic treatment target in pituitary adenomas
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Müller, A. (2015) · edoc Publication server (Humboldt University of Berlin) · doi:10.18452/17284 · Cited by 1
Mammalian Target of Rapamycin in Renal Cell Carcinoma
Jonasch, E., & Choueiri, M. (2012) · Humana Press eBooks · doi:10.1007/978-1-62703-062-5_18
Dou, Q. P. (2006) · Defense Technical Information Center (DTIC)
Kloke, O., Kummer, G., & Seeber, S. (1997) · Immunology Letters · doi:10.1016/s0165-2478(97)00110-7
Recent advances in immunosuppression
Woude, A. C. V., & Bierer, B. E. (1995) · Seminars in Anesthesia Perioperative Medicine and Pain · doi:10.1016/s0277-0326(05)80011-x
The Role of mTOR Inhibitors and P13K Pathway Blockade in RCC
Choueiri, M., & Mack, P. C. (2011) · Springer eBooks · doi:10.1007/978-3-642-21858-3_14
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Berlin, V., & Chiu, M. I. (1996) · Springer eBooks · doi:10.1007/978-3-642-57783-3_13
Cerovac, V., Garcia, J. L. M., Rubinfeld, H., Buchfelder, M., Losa, M., Florio, T., Páez-Pereda, M., Stalla, G. K., & Theodoropoulou, M. (2023) · doi:10.1158/0008-5472.c.6500775
Liao, K., Guo, C., Zhang, M., Lv, X., & Yan, J. (2025) · Leukemia Research Reports · doi:10.1016/j.lrr.2025.100558
(2011) · International Journal of Phytomedicine · Cited by 203
Metadata
- Authors
- Albers, M. W., Williams, R. T., Brown, E. J., Tanaka, A., Hall, F. L., & Schreiber, S. L.
- Affiliations
- Harvard University (US)
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1993-10
- Volume
- 268
- Issue
- 30
- Pages
- 22825-22829
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1485463834
- Cited by
- 145
- References
- 26
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 3 · 2015: 5 · 2014: 1 · 2013: 3 · 2012: 3
- FWCI
- 5.41
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM38627)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
- Keywords
- rapamycin, cyclin-dependent kinases, cyclin D1, G1 cell cycle arrest, osteosarcoma, MG-63 cells, cell cycle regulation, FK506
- MeSH terms
- Carrier Proteins, Heat-Shock Proteins, Protamine Kinase, Humans, Immunosuppressive Agents, Polyenes, Protein Binding, Osteosarcoma, Tumor Cells, Cultured, G1 Phase, S Phase, Cyclins, Protein Serine-Threonine Kinases, Cyclin-Dependent Kinases, Sirolimus, Tacrolimus Binding Proteins, CDC2-CDC28 Kinases, Protein Kinase Inhibitors, Cyclin-Dependent Kinase 2
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