Cited by 353Genomics, phytochemicals, and oxidative stressBioactive Compounds and Antitumor AgentsPharmacogenetics and Drug Metabolism

Regulation of human NAD(P)H:quinone oxidoreductase gene. Role of AP1 binding site contained within human antioxidant response element.

Open access
Li, Y, Jaiswal, A.K.·1992·Journal of Biological Chemistry

Abstract

Deletion mutagenesis and transfection studies into hepatic (mouse hepatoma (Hepa-1) and human hepatoblastoma (Hep-G2)) and nonhepatic (HeLa) cells indicated that high levels of expression of the human NAD(P)H:quinone oxidoreductase gene in tumor cells and its induction by beta-naphthoflavone and 3-(2)-tert-butyl-4-hydroxyanisole are mediated by human antioxidant response element (hARE) located in the region between -470 and -445. The hARE, when attached to the thymidine kinase promoter and transfected into several mammalian cells, expressed high levels of the chloramphenicol acetyltransferase gene that was inducible by beta-naphthoflavone and 3-(2)-tert-butyl-4-hydroxyanisole. Nucleotide sequence analysis of the hARE revealed the presence of a recognition site for binding to the AP1 protein. Mutation of the AP1 binding site located within the hARE resulted in the loss of expression and induction upon transfection into various cell types. Band shift and competition assays with hARE and nuclear extracts from control and beta-naphthoflavone-treated Hepa-1, Hep-G2 and HeLa cells indicated specific interaction of regulatory protein(s) to the hARE. The supershift assays using antibodies against specific proteins of the AP1 family identified Jun-D and c-Fos as two members in the hARE-protein complex observed in band shift assays.

Integrity signals

Registry

Crossref · 10.1016/s0021-9258(18)42151-5

Cites

38 of 38 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Li, Y., & Jaiswal, A. K.
Affiliations
Fox Chase Cancer Center (US)
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1992-07
Volume
267
Issue
21
Pages
15097-15104
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1603378617
Cited by
353
References
38
Citations by year
2026: 2 · 2025: 1 · 2022: 4 · 2021: 3 · 2020: 4 · 2019: 4 · 2018: 5 · 2017: 6 · 2016: 4 · 2015: 11 · 2014: 11 · 2013: 5 · 2012: 6
FWCI
7.54
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
National Institute of General Medical Sciences (GM-47466)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Bioactive Compounds and Antitumor Agents
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Pharmacogenetics and Drug Metabolism
Keywords
NAD(P)H:quinone oxidoreductase, antioxidant response element, AP-1 binding site, JunD, c-fos, beta-naphthoflavone, gene induction, transcriptional regulation, hepatoma cells, hepatoblastoma cells, chloramphenicol acetyltransferase reporter
MeSH terms
Amino Acid Sequence, Animals, Antioxidants, Benzoflavones, Binding Sites, Butylated Hydroxyanisole, Enzyme Induction, Glutathione Transferase, HeLa Cells, Humans, Molecular Sequence Data, Plasmids, Sequence Homology, Nucleic Acid, Transfection, Tumor Cells, Cultured, Chloramphenicol O-Acetyltransferase, Gene Expression Regulation, Enzymologic, Mutagenesis, NAD(P)H Dehydrogenase (Quinone), Proto-Oncogene Proteins c-jun, Proto-Oncogene Proteins c-fos, beta-Naphthoflavone, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review