The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
Transforming growth factor-beta induction of type-1 plasminogen activator inhibitor. Pericellular deposition and sensitivity to exogenous urokinase.
Open accessAbstract
The human tumor cell line HT-1080 was used as a model system to study the effects of transforming growth factor-beta (TGF beta) on polypeptide synthesis and proteolytic activity of malignant cells. Confluent cultures were exposed to TGF beta under serum-free conditions, and alterations in the production of proteins were examined by metabolic labeling and polypeptide analysis. TGF beta induced the synthesis and secretion of the Mr 47,000 endothelial type plasminogen activator inhibitor (PAI-1) as shown by reverse zymography, immunblotting, and immunoprecipitation analyses. TGF beta-induced PAI-1 was rapidly deposited in the growth substratum of the cells as shown by metabolic labeling and extraction of the cultures with sodium deoxycholate. Using pulse-chase experiments, we found a relatively fast turnover of substratum-associated PAI-1. Exogenously added urokinase released PAI-1 from the substratum even in the presence of the plasmin inhibitor aprotinin, suggesting a direct effect of urokinase. Immunoreactive complexes of higher molecular weight were subsequently detected in the medium. Epidermal growth factor, transforming growth factor-alpha, platelet-derived growth factor, and insulin did not elicit similar effects on the amount of PAI-1. TGF beta also inhibited the anchorage-independent growth of HT-1080 cells at the same concentrations at which it induced PAI-1. These results indicate that TGF beta can modulate the extracellular proteolytic activity of cultured cells by enhancing the secretion and deposition of PAI-1 into their microenvironment. It remains to be established whether TGF beta inhibition of anchorage-independent growth of these cells is associated with the induction of PAI-1.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Towbin, H., Staehelin, T., & Gordon, J. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.9.4350 · Cited by 53,385
Roberts, A. B., Sporn, M. B., Assoian, R. K., Smith, J. M., Roche, N. S., Wakefield, L. M., Heine, U. I., Liotta, L. A., Falanga, V, & Kehrl, J. H. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.12.4167 · Cited by 2,866
Plasminogen Activators, Tissue Degradation, and Cancer
(1985) · Advances in Cancer Research · doi:10.1016/s0065-230x(08)60028-7 · Cited by 2,535
Ignotz, R. A., & Massagué, J. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)35666-1 · Cited by 2,492
Derynck, R., Jarrett, J. A., Chen, E. Y., Eaton, D. H., Bell, J. R., Assoian, R. K., Roberts, A. B., Sporn, M. B., & Goeddel, D. V. (1985) · Nature · doi:10.1038/316701a0 · Cited by 1,667
Growth factors from murine sarcoma virus-transformed cells.
de Larco, J. E., & Todaro, G. J. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.8.4001 · Cited by 1,461
Role of collagenous matrices in the adhesion and growth of cells.
Kleinman, H. K., Klebe, R. J., & Martin, G. R. (1981) · The Journal of cell biology · doi:10.1083/jcb.88.3.473 · Cited by 1,361
Autocrine Secretion and Malignant Transformation of Cells
Sporn, M. B., & Todaro, G. J. (1980) · New England Journal of Medicine · doi:10.1056/nejm198010093031511 · Cited by 1,346
Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli
Pennica, D., Holmes, W. E., Kohr, W. J., Harkins, R. N., Vehar, G. A., Ward, C. A., Bennett, W. F., Yelverton, E., Seeburg, P. H., Heyneker, H. L., et al. (1983) · Nature · doi:10.1038/301214a0 · Cited by 1,243
Cited by
Yamamoto, T., Nakamura, T., Noble, N. A., Ruoslahti, E., & Border, W. A. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.5.1814 · Cited by 830
Cell-Associated Plasminogen Activation: Regulation and Physiological Functions
Saksela, O., & Rifkin, D. B. (1988) · Annual Review of Cell Biology · doi:10.1146/annurev.cb.04.110188.000521 · Cited by 818
Self-citationThe Transforming Growth Factor-βs
Roberts, A. B., & Sporn, M. B. (1990) · Handbook of Experimental Pharmacology · doi:10.1007/978-3-642-49295-2_8 · Cited by 746
92-kD type IV collagenase mediates invasion of human cytotrophoblasts.
Librach, C. L., Werb, Z., Fitzgerald, M. L., Chiu, K., Corwin, N. M., Esteves, R. A., Grobelny, D., Galardy, R., Damsky, C. H., & Fisher, S. J. (1991) · The Journal of cell biology · doi:10.1083/jcb.113.2.437 · Cited by 720
Vukicevic, S., Kleinman, H. K., Luyten, F. P., Roberts, A. B., Roche, N. S., & Reddi, A. H. (1992) · Experimental Cell Research · doi:10.1016/0014-4827(92)90397-q · Cited by 696
Metadata
- Authors
- Laiho, M., Saksela, O., & Keski-Oja, J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1987-12
- Volume
- 262
- Issue
- 36
- Pages
- 17467-17474
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1497695953
- Cited by
- 257
- References
- 51
- FWCI
- 6.60
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Transforming growth factor-beta induction of type-1 plasminogen activator inhibitor. Pericellular deposition and sensitivity to exogenous urokinase. https://app.cochat.ai/papers/10.1016/s0021-9258(18)45403-8
- The paper
- Laiho, M., Saksela, O., & Keski-Oja, J. (1987). Transforming growth factor-beta induction of type-1 plasminogen activator inhibitor. Pericellular deposition and sensitivity to exogenous urokinase. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)45403-8
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
