The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Identification of a stromelysin cleavage site within the interglobular domain of human aggrecan. Evidence for proteolysis at this site in vivo in human articular cartilage.
Open accessAbstract
Products generated by the digestion of human aggrecan with recombinant human stromelysin have been purified and analyzed by N-terminal sequencing and C-terminal peptide isolation. N-terminal analysis of chondroitin sulfate-bearing fragments revealed a clearly identifiable sequence initiating at residue Phe342 of human aggrecan, providing evidence for a cleavage site at the Asn341-Phe342 bond located within the interglobular domain. This cleavage site, which separates the G1 domain from the remainder of the molecule, was confirmed by isolation from the liberated G1 domain of a C-terminal tryptic peptide with the sequence YDAICYTGEDFVDIPEN (in which the C-terminal residue is Asn341). This peptide was also isolated from tryptic digests of hyaluronan-binding proteins (A1D4 samples) prepared by CsCl gradient centrifugation of extracts of mature human articular cartilages. Since these A1D4 samples contain G1 domain which accumulates as a result of aggrecan catabolism in vivo, these results clearly indicate that stromelysin cleaves the Asn341-Phe342 bond of human aggrecan in situ.
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Cites
A Direct Spectrophotometric Microassay for Sulfated Glycosaminoglycans in Cartilage Cultures
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Murphy, G., Cockett, M. I., Ward, R. V., & Docherty, A. J. P. (1991) · Biochemical Journal · doi:10.1042/bj2770277 · Cited by 457
Doege, K. J., Sasaki, M., Kimura, T., & Yamada, Y. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)35257-2 · Cited by 446
Comparison of human stromelysin and collagenase by cloning and sequence analysis
Whitham, S. E., Murphy, G., Angel, P., Rahmsdorf, H. J., Smith, B. J., Lyons, A., Harris, T. J. R., Reynolds, J. J., Herrlich, P., & Docherty, A. J. P. (1986) · Biochemical Journal · doi:10.1042/bj2400913 · Cited by 436
Wilhelm, S. M., Collier, I. E., Kronberger, A., Eisen, A. Z., Marmer, B. L., Grant, G. A., Bauer, E. A., & Goldberg, G. I. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.19.6725 · Cited by 321
Sandy, J. D., Neame, P. J., Boynton, R. E., & Flannery, C. R. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)31499-6 · Cited by 284
Self-citationSites of stromelysin cleavage in collagen types II, IX, X, and XI of cartilage.
Wu, J. J., Lark, M. W., Chun, L. E., & Eyre, D. R. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)67640-4 · Cited by 259
Self-citationProteoglycans of articular cartilage: Changes in aging and in joint disease
Hardingham, T., & Bayliss, M. (1990) · Seminars in Arthritis and Rheumatism · doi:10.1016/0049-0172(90)90044-g · Cited by 232
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Self-citationCleavage of cartilage proteoglycan between G1 and G2 domains by stromelysins
Fosang, A. J., Neame, P. J., Hardingham, T. E., Murphy, G., & Hamilton, J. A. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)98442-5 · Cited by 166
Cited by
Cloning and Characterization of ADAMTS11 , an Aggrecanase from the ADAMTS Family
Abbaszade, I., Liu, R.-Q., Yang, F., Rosenfeld, S. A., Ross, O. H., Link, J. R., Ellis, D. M., Tortorella, M. D., Pratta, M. A., Hollis, J. M., et al. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.33.23443 · Cited by 490
Baillat, D., Bègue, A., Stéhelin, D., & Aumercier, M. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m200088200 · Cited by 84
Increased metalloproteinase activity in the hippocampus following status epilepticus
Dubey, D., McRae, P. A., Rankin-Gee, E. K., Baranov, E., Wandrey, L., Rogers, S., & Porter, B. E. (2017) · Epilepsy Research · doi:10.1016/j.eplepsyres.2017.02.021 · Cited by 65
Mercuri, F. A., Maciewicz, R. A., Tart, J., Last, K., & Fosang, A. J. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.42.33038 · Cited by 52
Madsen, S. H., Sumer, E. U., Bay-Jensen, A.-C., Sondergaard, B.-C., Qvist, P., & Karsdal, M. A. (2010) · Biomarkers · doi:10.3109/13547500903521810 · Cited by 29
Metadata
- Authors
- Flannery, C. R., Lark, M. W., & Sandy, J. D.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1992-01
- Volume
- 267
- Issue
- 2
- Pages
- 1008-1014
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1494656326
- Cited by
- 270
- References
- 13
- FWCI
- 8.59
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Flannery, C. R., Lark, M. W., & Sandy, J. D. (1992). Identification of a stromelysin cleavage site within the interglobular domain of human aggrecan. Evidence for proteolysis at this site in vivo in human articular cartilage. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)48387-1
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