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Cited by 138

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Three direct repeats and a TATA-like sequence are required for regulated expression of the human low density lipoprotein receptor gene

Südhof, T C, Van der Westhuyzen, D R, Goldstein, J L, Brown, M S, Russell, D W·1987·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(18)61030-0
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Abstract

The low density lipoprotein receptor is encoded by a "housekeeping" gene that is transcribed in most mammalian cells and is subject to negative feedback regulation by sterols. To determine the basis for this regulated expression, we performed a transfection analysis with hybrid genes containing up to 6500 base pairs of 5' flanking DNA from the low density lipoprotein receptor gene fused to the coding region of the bacterial chloramphenicol acetyltransferase gene. These studies identified a 177-base pair fragment of 5' flanking DNA that is sufficient for expression as well as negative regulation by sterols. The positive elements within this region were further defined by analysis of a series of 15 mutations in which overlapping 10-base pair segments were scrambled by site-specific mutagenesis. These studies identified the positive elements as three imperfect direct repeats of 16 base pairs and a TATA-like sequence. The three repeats contain a sequence that is homologous to the consensus DNA sequence recognized by transcription factor Sp1.

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Crossref · 10.1016/s0021-9258(18)61030-0

Cites

1 of 1 references verified0 citation errors · 0 not checkable

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Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Repeats 1 and 3 contain sequences recognized by Sp1 and function to maintain basal LDLR gene transcription regardless of sterols.

    “Repeats 1 and 3 contain sequences that are recognized by the general transcription factor specificity protein 1 (Sp1) and function to maintain basal LDLR gene transcription, regardless of the presence or absence of sterols19,20.”

    From Pathways and Molecular Mechanisms Governing LDL Receptor Regulation (2025) · cites this paper as [19]

  • The LDLR gene promoter contains three imperfect direct 16 bp repeats, two TATA-like sequences, and multiple transcription initiation sites, all required for gene expression and regulation.

    “The promoter region contains three imperfect direct 16 bp repeats, two TATA-like sequences, and multiple transcription initiation sites, which are all required for gene expression and regulation19”

    From Pathways and Molecular Mechanisms Governing LDL Receptor Regulation (2025) · cites this paper as [19]

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 31

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Metadata

Authors
Südhof, T. C., Van der Westhuyzen, D. R., Goldstein, J. L., Brown, M. S., & Russell, D. W.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1987-08
Volume
262
Issue
22
Pages
10773-10779
ISSN
0021-9258
DOI
10.1016/s0021-9258(18)61030-0
Cited by
138
References
1
Language
en
Licence

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