The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Two Different Cell Types have Different Major Receptors for Human Tumor Necrosis Factor (TNFα)
Open accessAbstract
The receptors for tumor necrosis factor alpha (TNF alpha) were analyzed on myeloid cells (HL60, U937, K562, and freshly isolated blood monocytes) and on cells of epithelial origin (MCF7, HEp2 and HeLa cells), by use of radiolabeled TNF alpha and cross-linking experiments. Both cell types had high but slightly different affinities for TNF alpha. The myeloid cells had major cross-linked products of 98-100 kDa, which were similar in their N-linked glycosylation, whereas the cells of epithelial origin contained a major cross-linked product of 75 kDa, a second product of 95 kDa. The major receptors of both cell types (studied mostly with HL60 and HEp2 cells) are different proteins because (a) their apparent molecular masses were different and no evidence was obtained for cell-specific proteases, which could generate the differently sized receptors from one common receptor molecule; (b) anti-receptor antibodies, which precipitated the 95- and 75-kDa products, did not precipitate the 100-kDa cross-linked complex; (c) the native TNF alpha-receptor complexes had different proteolytic fingerprints; (d) the tryptic fragments differed in their association with the cell membrane vesicles; (e) the receptors differed in their degree of N-linked glycosylation; and (f) O-linked glycosylation was found on the major receptor of HL60 but not of HEp2 cells. In addition, myeloid cells may also contain a small amount of the HEp2-type of TNF alpha receptor. We suggest that at least two different receptors for TNF alpha exist.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Fraker, P. J., & Speck, J. C. (1978) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(78)91322-0 · Cited by 4,558
An endotoxin-induced serum factor that causes necrosis of tumors
Carswell, E. A., Old, L. J., Kassel, R. L., Green, S., Fiore, N., & Williamson, B. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.9.3666 · Cited by 4,532
Silver staining of proteins in polyacrylamide gels
Wray, W., Boulikas, T., Wray, V. P., & Hancock, R. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90179-2 · Cited by 3,504
Cachectin and tumour necrosis factor as two sides of the same biological coin
Beutler, B., & Cerami, A. (1986) · Nature · doi:10.1038/320584a0 · Cited by 1,647
Cachectin: More Than a Tumor Necrosis Factor
Epstein, F. H., Beutler, B., & Cerami, A. (1987) · New England Journal of Medicine · doi:10.1056/nejm198702123160705 · Cited by 1,432
Tumor Necrosis Factor (Cachectin) as an Essential Mediator in Murine Cerebral Malaria
Grau, G. E., Fajardo, L. F., Piguet, P.-F., Allet, B., Lambert, P.-H., & Vassalli, P. (1987) · Science · doi:10.1126/science.3306918 · Cited by 736
Molecular Cloning of the Complementary DNA for Human Tumor Necrosis Factor
Wang, A. M., Creasey, A. A., Ladner, M. B., Lin, L. S., Strickler, J., Van Arsdell, J. N., Yamamoto, R., & Mark, D. F. (1985) · Science · doi:10.1126/science.3856324 · Cited by 622
Structure of tumour necrosis factor
Jones, E. Y., Stuart, D. I., & Walker, N. P. C. (1989) · Nature · doi:10.1038/338225a0 · Cited by 505
Ranges, G. E., Figari, I. S., Espevik, T., & Palladino, M. A. (1987) · The Journal of experimental medicine · doi:10.1084/jem.166.4.991 · Cited by 494
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Itoh, N., Yonehara, S., Ishii, A., Yonehara, M., Mizushima, S.-I., Sameshima, M., Hase, A., Seto, Y., & Nagata, S. (1991) · Cell · doi:10.1016/0092-8674(91)90614-5 · Cited by 2,737
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Schall, T. J., Lewis, M., Koller, K. J., Lee, A., Rice, G. C., Wong, G. H. W., Gatanaga, T., Granger, G. A., Lentz, R., Raab, H., et al. (1990) · Cell · doi:10.1016/0092-8674(90)90816-w · Cited by 964
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Smith, C. A., Davis, T., Anderson, D., Solam, L., Beckmann, M. P., Jerzy, R., Dower, S. K., Cosman, D., & Goodwin, R. G. (1990) · Science · doi:10.1126/science.2160731 · Cited by 959
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Loetscher, H., Pan, Y.-C. E., Lahm, H.-W., Gentz, R., Brockhaus, M., Tabuchi, H., & Lesslauer, W. (1990) · Cell · doi:10.1016/0092-8674(90)90815-v · Cited by 905
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Metadata
- Authors
- Hohmann, H. P., Remy, R., Brockhaus, M., & van Loon, A. P.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1989-09
- Volume
- 264
- Issue
- 25
- Pages
- 14927-14934
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1552669957
- Cited by
- 356
- References
- 27
- FWCI
- 18.07
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Hohmann, H. P., Remy, R., Brockhaus, M., & van Loon, A. P. (1989). Two Different Cell Types have Different Major Receptors for Human Tumor Necrosis Factor (TNFα). Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)63791-3
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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