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Cited by 273

The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.

Actin polymerization. The mechanism of action of cytochalasin D.

Open access
Goddette, D W, Frieden, C·1986·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(18)66662-1
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Abstract

Fluorescence changes using actin covalently labeled with N-(1-pyrenyl)iodoacetamide have been used to determine the effect of cytochalasin D on actin polymerization. A mechanism for the effect of cytochalasin D on actin polymerization is presented, which explains the experimental observation of a cytochalasin D-induced increase in the initial rate of polymerization and a decrease in the final extent of the reaction. Central to this mechanism is the Mg2+-dependent formation of cytochalasin D-induced dimers. The dimers serve as nuclei to enhance the polymerization rate. Binding of Mg2+ to a low affinity site on the dimer induces a conformational change which can be observed as a rapid fluorescence increase. A subsequent time-dependent fluorescence decrease observed prior to polymerization appears to represent ATP hydrolysis resulting in dissociation of the dimer and release of actin monomers containing ADP. We postulate that a slow rate of exchange of ATP for bound ADP relative to hydrolysis results in the accumulation of monomers containing ADP. As these monomers have a high critical concentration, the final extent of polymerization is reduced dramatically. The Mg2+ dependence of the final extent of polymerization in the presence of cytochalasin D is also explained in the context of this mechanism.

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Metadata

Authors
Goddette, D. W., & Frieden, C.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1986-12
Volume
261
Issue
34
Pages
15974-15980
ISSN
0021-9258 (ISSN-L)
DOI
10.1016/s0021-9258(18)66662-1
OpenAlex
W1549239847
Cited by
273
References
27
FWCI
3.19
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Actin polymerization. The mechanism of action of cytochalasin D. https://app.cochat.ai/papers/10.1016/s0021-9258(18)66662-1
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Goddette, D. W., & Frieden, C. (1986). Actin polymerization. The mechanism of action of cytochalasin D. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)66662-1

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.