The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
A single amino acid substitution in rhodopsin (lysine 248—-leucine) prevents activation of transducin.
Open accessAbstract
In structure-function studies on bovine rhodopsin by in vitro site-specific mutagenesis, we have prepared three mutants in the cytoplasmic loop between the putative transmembrane helices E and F. In each mutant, charged amino acid residues were replaced by neutral residues: mutant 1, Glu239----Gln; mutant 2, Lys248----Leu; and mutant 3, Glu247----Gln, Lys248----Leu, and Glu249----Gln. The mutant rhodopsin genes were expressed in monkey kidney (COS-1) cells. After the addition of 11-cis-retinal to the cells, the rhodopsin mutants were purified by immunoaffinity adsorption. Each mutant gave a wild-type rhodopsin visible absorption spectrum. The mutants were assayed for their ability to stimulate the GTPase activity of transducin in a light-dependent manner. While mutants 1 and 3 showed wild-type activity, mutant 2 (Lys248----Leu) was inactive.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 317,331
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
Burnette, W. N. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90281-5 · Cited by 8,429
Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity
HUNTER, W. M., & GREENWOOD, F. C. (1962) · Nature · doi:10.1038/194495a0 · Cited by 6,919
Silver staining of proteins in polyacrylamide gels
Wray, W., Boulikas, T., Wray, V. P., & Hancock, R. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90179-2 · Cited by 3,504
Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs
Kozak, M. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.2.857 · Cited by 3,293
Assay of proteins in the presence of interfering materials
Bensadoun, A., & Weinstein, D. (1976) · Analytical Biochemistry · doi:10.1016/s0003-2697(76)80064-4 · Cited by 3,179
Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
Biggin, M. D., Gibson, T. J., & Hong, G. F. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.13.3963 · Cited by 2,376
Molecular Genetics of Human Color Vision: The Genes Encoding Blue, Green, and Red Pigments
Nathans, J., Thomas, D., & Hogness, D. S. (1986) · Science · doi:10.1126/science.2937147 · Cited by 1,721
Cited by
Savarese, T. M., & Fraser, C. M. (1992) · Biochemical Journal · doi:10.1042/bj2830001 · Cited by 473
Karnik, S. S., Sakmar, T. P., Chen, H. B., & Khorana, H. G. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.22.8459 · Cited by 414
Self-citationRhodopsin Mutants That Bind But Fail to Activate Transducin
Franke, R. R., König, B., Sakmar, T. P., Khorana, H. G., & Hofmann, K. P. (1990) · Science · doi:10.1126/science.2218504 · Cited by 377
Self-citationCloning and expression of a novel angiotensin II receptor subtype.
Sandberg, K., Ji, H., Clark, A. J., Shapira, H., & Catt, K. J. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)50109-0 · Cited by 274
Metadata
- Authors
- Franke, R. R., Sakmar, T. P., Oprian, D. D., & Khorana, H. G.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1988-02
- Volume
- 263
- Issue
- 5
- Pages
- 2119-2122
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1579091371
- Cited by
- 259
- References
- 25
- FWCI
- 5.46
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A single amino acid substitution in rhodopsin (lysine 248—-leucine) prevents activation of transducin. https://app.cochat.ai/papers/10.1016/s0021-9258(18)69178-1
- The paper
- Franke, R. R., Sakmar, T. P., Oprian, D. D., & Khorana, H. G. (1988). A single amino acid substitution in rhodopsin (lysine 248—-leucine) prevents activation of transducin. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)69178-1
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
