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Identification of the acidic and basic subunit complexes of glycinin.
Open accessAbstract
Five complexes consisting of one acidic and one basic subunit that were linked via disulfide bonds were purified from unreduced S-alkylated glycinin. The acidic and basic subunits were identified unambiguously using NH2-terminal sequence analysis, sodium dodecyl sulfate (SDS)-electrophoresis, and analytical isoelectric focusing. The subunit pairings are A1aB2, A1bB1b, A2B1a, A3B4, and F2(2)B3. Polypeptide A4 was not linked to a corresponding basic subunit via a disulfide bond. The study shows that pairing between subunits is nonrandom, which is consistent with evidence that glycinin is synthesized as a Mr = 60,000 precursor that undergoes post-translational modification to form the individual linked subunits.
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Cited by
Characterization of the glycinin gene family in soybean.
Nielsen, N. C., Dickinson, C. D., Cho, T. J., Thanh, V. H., Scallon, B. J., Fischer, R. L., Sims, T. L., Drews, G. N., & Goldberg, R. B. (1989) · The Plant Cell · doi:10.1105/tpc.1.3.313 · Cited by 382
Self-citationChemistry and Nutritional Value of Soybean Components
Liu, K. (1997) · Soybeans · doi:10.1007/978-1-4615-1763-4_2 · Cited by 259
Huang, L., Ding, X., Li, Y., & Ma, H. (2019) · Food Chemistry · doi:10.1016/j.foodchem.2018.11.147 · Cited by 224
Mixing plant-based proteins: Gel properties of hemp, pea, lentil proteins and their binary mixtures
Guidi, S., Formica, F. A., & Denkel, C. (2022) · Food Research International · doi:10.1016/j.foodres.2022.111752 · Cited by 83
Metadata
- Authors
- Staswick, P. E., Hermodson, M. A., & Nielsen, N. C.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1981-08
- Volume
- 256
- Issue
- 16
- Pages
- 8752-8755
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1544745708
- Cited by
- 179
- References
- 0
- FWCI
- 3.55
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Staswick, P. E., Hermodson, M. A., & Nielsen, N. C. (1981). Identification of the acidic and basic subunit complexes of glycinin. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)68908-8
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