Amyloid-like properties of peptides flanking the epitope of amyloid precursor protein-specific monoclonal antibody 22C11.
Open accessAbstract
Alzheimer's disease is one of the prevalent forms of human dementia. Its pathology is distinguished by proteinaceous deposits ("amyloid") in the brain. They contain a peptide (beta A4) that is proteolytically derived from a larger transmembrane protein. To follow the different metabolic pathways of this Amyloid Precursor Protein (APP) may thus lead to the elucidation of the molecular basis of Alzheimer's disease. Specific antibodies are necessary tools for this task. Using synthetic peptides, we have characterized the epitope of the APP-specific monoclonal antibody 22C11; it is localized between residues 66 and 81 of APP. Some of the peptides flanking this site exhibited properties generally associated with amyloid, i.e. low solubility, filament formation, and birefringence after Congo Red staining. Exploiting differences in the peptides' aggregational properties, we present evidence that the two dyes Eosin and Direct Red 254, in conjunction with classical amyloid staining by Congo Red, can be used to characterize aggregating, amyloid-like peptides in vitro.
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Aβ1-42 forms insoluble amyloid fibrils more rapidly than Aβ1-40.
“A{31-42 has been shown to form insoluble amyloid fibrils more rapidly than A{31-40 (10)(11)(12)(13).”
From Amyloid β Protein (Aβ) in Alzheimeri's Disease Brain (1995) · cites this paper as [10]
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The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor
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Self-citationRETRACTED ARTICLE: APP binds DR6 to trigger axon pruning and neuron death via distinct caspases
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- Authors
- Hilbich, C., Mönning, U., Grund, C., Masters, C. L., & Beyreuther, K.
- Affiliations
- Heidelberg University (DE)
- ORCID
- Masters, C L
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1993-12
- Volume
- 268
- Issue
- 35
- Pages
- 26571-26577
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1484988735
- Cited by
- 137
- References
- 2
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 1 · 2022: 2 · 2021: 2 · 2019: 1 · 2017: 1 · 2015: 1 · 2014: 4 · 2013: 3 · 2012: 5
- FWCI
- 5.77
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Keywords
- amyloid precursor protein, epitope mapping, peptide aggregation, Alzheimer's disease, Congo red staining, eosin staining, Congo red birefringence
- MeSH terms
- Amino Acid Sequence, Amyloid, Antibodies, Monoclonal, Epitopes, Humans, Microscopy, Electron, Molecular Sequence Data, Peptides, Amyloid beta-Protein Precursor
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