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Studies on Squalene Epoxidase of Rat Liver
Open accessAbstract
Rat liver microsomes previously heated to 50" for 5 mm accumulate 2,3-oxidosqualene on incubation with squalene.Squalene epoxidase activity can be assayed either with squalene and heated microsomes or with lO,ll-dihydrosqualene and intact microsomes.In common with other monooxygenases, the epoxidase requires TPNH and molecular oxygen.Both a soluble fraction of rat liver and microsomes are necessary for enzyme activity.Carbon monoxide or potassium cyanide fall to inhibit squalene epoxidation.
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“Cholesterol homeostasis is maintained by negative feedback regulation of genes and their encoded enzymes in the pathway, including the rate limiting enzymes HMG CoA reductase (HMGCR) and SQLE20–22.”
From A suppressor screen in Mecp2 mutant mice implicates cholesterol metabolism in Rett syndrome (2013) · cites this paper as [22]
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Metadata
- Authors
- Yamamoto, S., & Bloch, K.
- Affiliations
- Harvard University (US)
- ORCID
- Bloch, Konrad
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1970-04
- Volume
- 245
- Issue
- 7
- Pages
- 1670-1674
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1606948338
- Cited by
- 163
- References
- 18
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 1 · 2016: 1 · 2015: 3 · 2014: 3 · 2013: 3 · 2012: 3
- FWCI
- 2.98
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Pharmacogenetics and Drug Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Cholesterol and Lipid Metabolism
- Keywords
- squalene epoxidase, squalene epoxidation, 2,3-oxidosqualene, liver microsomes, mixed function oxidase, molecular oxygen, enzyme inhibition, carbon monoxide, potassium cyanide, rat liver
- MeSH terms
- Alkenes, Animals, Carbon Isotopes, Carbon Monoxide, Chelating Agents, Cholestanes, Chromatography, Thin Layer, Cyanides, Ethers, Cyclic, Fatty Acids, Female, Lipoproteins, Liver, Microsomes, Liver, Oxygenases, Solubility, Squalene, Temperature, Transferases, Tritium, Rats
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