Cited by 344Cell Adhesion Molecules ResearchBiochemical and Structural CharacterizationPolymer Surface Interaction Studies

Selection of peptides binding to the alpha 5 beta 1 integrin from phage display library.

Open access
Koivunen, E, Gay, D.A., Ruoslahti, E·1993·Journal of Biological Chemistry

Abstract

The alpha 5 beta 1 integrin binds fibronectin through the integrin recognition sequence Arg-Gly-Asp (RGD). We have used a 6-amino acid peptide library expressed on filamentous phage to identify peptide ligands for alpha 5 beta 1. We found that this integrin selectively binds RGD-containing peptides from the library. Of the 32 different sequences obtained, 28 had the RGD motif, 3 contained sequences related to RGD, and only 1 had a clearly different sequence. One of the RGD-containing phage encoded a potentially cyclic insert CRGDCL. The cyclic peptide GAC*RGDC*LGA (where * denotes cysteines forming a disulfide bond) was 10-fold more efficient than any of the linear RGD-containing hexapeptides in inhibiting the binding of RGD-expressing phage to alpha 5 beta 1 or the attachment of alpha 5 beta 1-expressing cells to fibronectin. This peptide also inhibited cell attachment mediated by the alpha v beta 1, alpha v beta 3, and alpha v beta 5 integrins with about 10-fold higher efficiency than linear GRGDSP. One peptide containing an RGD-related sequence, NGRAHA, was also found to inhibit phage attachment and cell adhesion, especially adhesion mediated by the alpha v beta 5 integrin. These results indicate that novel and high affinity ligands for integrins can be isolated from a random peptide library.

Integrity signals

Registry

Crossref · 10.1016/s0021-9258(20)80715-7

Cites

62 of 62 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Koivunen, E., Gay, D. A., & Ruoslahti, E.
Affiliations
Sanford Burnham Prebys Medical Discovery Institute (US)
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1993-09
Volume
268
Issue
27
Pages
20205-20210
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1506729263
Cited by
344
References
62
Citations by year
2026: 2 · 2025: 6 · 2024: 4 · 2023: 8 · 2022: 3 · 2021: 8 · 2020: 5 · 2019: 2 · 2018: 6 · 2017: 8 · 2016: 15 · 2015: 8 · 2014: 9 · 2013: 11 · 2012: 14
FWCI
7.09
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5p01ca028896-15, 5r35ca042507-07)
  • National Cancer Institute (CA30199, CA 28896, CA-42507, P30CA030199)
Topics
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
  • Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
Keywords
alpha5beta1 integrin, RGD peptide, fibronectin, integrin ligands, cyclic RGD peptide, phage display, cell adhesion, peptide library screening
MeSH terms
Amino Acid Sequence, Animals, Bacteriophages, Base Sequence, Binding, Competitive, Fibronectins, Cricetinae, Kinetics, Molecular Sequence Data, Oligodeoxyribonucleotides, Oligopeptides, Transfection, Gene Library, Integrins, Polymerase Chain Reaction, Databases, Factual, CHO Cells, Receptors, Fibronectin

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review