High-performance liquid chromatography coupled to nuclear magnetic resonance spectroscopy
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The protic solvent problem was overcome by using deuterated water in the mobile phase of RP-HPLC, and the sensitivity problem by using a stopped-flow method.
“The former was overcome by using deuterated water in the mobile phase of the RP-HPLC and the latter by using a stopped-flow method to allow adequate time to obtain adequate (if not complete) 1 H-spectra in 10 min of stopped-flow with ca. 100 µg of reference ecdysteroids [137].”
From Ecdysteroids (2021) · cites this paper as [137]
Cites
On-line use of NMR detection in separation chemistry
Albert, K. (1995) · Journal of Chromatography A · doi:10.1016/0021-9673(95)00239-j · Cited by 166 · see citing works
Direct coupling of chromatographic separations to NMR spectroscopy
Lindon, J. C., Nicholson, J. K., & Wilson, I. D. (1996) · Progress in Nuclear Magnetic Resonance Spectroscopy · doi:10.1016/0079-6565(95)01027-0 · Cited by 136 · see citing works
Self-citationWolfender, J., Rodriguez, S., Hostettmann, K., & Hiller, W. (1997) · Phytochemical Analysis · doi:10.1002/(sici)1099-1565(199705)8:3 · Cited by 57 · see citing works
Chromatographic procedures for phytoecdysteroids
Lafont, R., Morgan, E. D., & Wilson, I. D. (1994) · Journal of Chromatography A · doi:10.1016/0021-9673(94)85205-7 · Cited by 46 · see citing works
Self-citationThin-layer chromatography of ecdysteroids originated fromSilene otites L. (Wib.)
Báthori, M., Szendrei, K., Kalász, H., et al. (1988) · Chromatographia · doi:10.1007/bf02327660 · Cited by 11 · see citing works
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Lafont, R., Balducci, C., & Dinan, L. (2021) · Encyclopedia · doi:10.3390/encyclopedia1040096 · Cited by 49 · see citing works
Self-citationGlazowska (2018) · doi:10.1002/jssc.201800506 · Cited by 22 · see citing works
Lafont, R., Blais, C., Harmatha, J., & Wilson, I. D. (2013) · Reference Module in Chemistry, Molecular Sciences and Chemical Engineering · doi:10.1016/b978-0-12-409547-2.04726-0 · Cited by 1 · see citing works
Self-citation
- Authors
- Wilson, I. D., Morgan, E. D., Lafont, R., & Wright, B.
- Affiliations
- Keele University (GB)
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Wilson, I.D
- Venue
- Journal of Chromatography A
- Publisher
- Elsevier BV
- Published
- 1998-03
- Volume
- 799
- Issue
- 1-2
- Pages
- 333-336
- ISSN
- 0021-9673 (ISSN-L)
- OpenAlex
- W166716730
- Cited by
- 32
- References
- 5
- Citations by year
- 2025: 1 · 2022: 1 · 2021: 2 · 2019: 1 · 2018: 1 · 2017: 1 · 2013: 2 · 2012: 1
- FWCI
- 6.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Chromatography A (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Physical Sciences › Chemistry › Spectroscopy › Advanced NMR Techniques and Applications
- Keywords
- ecdysteroids, Silene otites, 20-hydroxyecdysone, 1H NMR spectrum, plant extracts, ecdysteroid biosynthesis
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
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