Chromatographic procedures for phytoecdysteroids
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SFC is compatible with UV/vis-, FT-IR and MS-detection.
“SFC is compatible with UV/vis-, FT-IRand MS-detection [107,117,129].”
From Ecdysteroids (2021) · cites this paper as [107]
Open-column chromatography used to be the standard workhorse for laboratory purification of ecdysteroids but has gradually been replaced by flash chromatography and preparative HPLC.
“Open-column chromatography on silica or alumina eluted with solvents of increasing percentages of methanol or ethanol in hexane, dichloromethane or chloroform used to be the standard 'workhorse' for the laboratory purification of ecdysteroids, as it was of other plant secondary compounds, but this has gradually been replaced by flash chromatography and preparative HPLC on NP-and RP-stationary”
From Ecdysteroids (2021) · cites this paper as [107]
SFC separation of ecdysteroids provides shorter retention times, sharper peaks and greater sensitivity than HPLC.
“SFC separation of ecdysteroids, using critical CO 2 , which is non-polar, modified with methanol and silica/bonded (NP) silica HPLC columns or fused silica capillary columns, provides shorter retention times, sharper peaks and greater sensitivity than HPLC.”
From Ecdysteroids (2021) · cites this paper as [107]
The most commonly used HPLC systems are variants on a C18-RP-column eluted with a linear gradient of methanol or acetonitrile in water.
“The most commonly used HPLC systems are variants on a C 18 -RP-column eluted with a linear gradient of methanol or acetonitrile in water.”
From Ecdysteroids (2021) · cites this paper as [107]
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Thin-layer chromatography of ecdysteroids originated fromSilene otites L. (Wib.)
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Self-citationQuantitative determination of phytoecdysones as illustrated by application to species of Helleborus
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Phytoecdysteroids: Biological aspects
Dinan, L. (2001) · Phytochemistry · doi:10.1016/s0031-9422(01)00078-4 · Cited by 392 · see citing works
The Phytochemical, Biological, and Medicinal Attributes of Phytoecdysteroids: An Updated Review
Das, N., Mishra, S. K., Bishayee, A., Ali, E. S., & Bishayee, A. (2021) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2020.10.012 · Cited by 115 · see citing works
Ecdysteroids: Production in plant in vitro cultures
Thiem, B., Kikowska, M., Maliński, M. P., Kruszka, D., Napierała, M., & Florek, E. (2017) · Phytochemistry Reviews · doi:10.1007/s11101-016-9483-z · Cited by 77 · see citing works
“There are also some other methods for ecdysteroids isolation, however, they are efficient only on an analytical scale (Morgan et al. 1990; Lafont et al. 1994).”
MethodLafont, R., Balducci, C., & Dinan, L. (2021) · Encyclopedia · doi:10.3390/encyclopedia1040096 · Cited by 49 · see citing works
Self-citationHigh-performance liquid chromatography coupled to nuclear magnetic resonance spectroscopy
Wilson, I. D., Morgan, E. D., Lafont, R., & Wright, B. (1998) · Journal of Chromatography A · doi:10.1016/s0021-9673(97)01299-5 · Cited by 32 · see citing works
Self-citationSelectivity in the high-performance liquid chromatography of ecdysteroids
Lafont, R., Kaouadji, N., Morgan, E. D., & Wilson, I. D. (1994) · Journal of Chromatography A · doi:10.1016/0021-9673(94)85206-5 · Cited by 28 · see citing works
Self-citationGlazowska (2018) · doi:10.1002/jssc.201800506 · Cited by 22 · see citing works
Ho, R., Girault, J.-P., Cousteau, P.-Y., Bianchini, J.-P., Raharivelomanana, P., & Lafont, R. (2008) · Journal of Chromatographic Science · doi:10.1093/chromsci/46.2.102 · Cited by 28 · see citing works
Lafont, R., Blais, C., Harmatha, J., & Wilson, I. D. (2013) · Reference Module in Chemistry, Molecular Sciences and Chemical Engineering · doi:10.1016/b978-0-12-409547-2.04726-0 · Cited by 1 · see citing works
Self-citation
- Authors
- Lafont, R., Morgan, E. D., & Wilson, I. D.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Keele University (GB)
- Corresponding author
- Morgan, E.D.
- Venue
- Journal of Chromatography A
- Publisher
- Elsevier BV
- Published
- 1994-01
- Volume
- 658
- Issue
- 1
- Pages
- 31-53
- ISSN
- 0021-9673 (ISSN-L)
- OpenAlex
- W1981361344
- Cited by
- 46
- References
- 78
- Citations by year
- 2024: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2013: 2
- FWCI
- 4.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Chromatography A (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical Studies and Bioactivities
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Keywords
- phytoecdysteroids, ecdysteroids, plant steroids, separation processes, chromatographic methods
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