Ecdysteroids: Chromatography
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SFC is compatible with UV/vis-, FT-IR and MS-detection.
“SFC is compatible with UV/vis-, FT-IRand MS-detection [107,117,129].”
From Ecdysteroids (2021) · cites this paper as [117]
The most commonly used HPLC systems are variants on a C18-RP-column eluted with a linear gradient of methanol or acetonitrile in water.
“The most commonly used HPLC systems are variants on a C 18 -RP-column eluted with a linear gradient of methanol or acetonitrile in water.”
From Ecdysteroids (2021) · cites this paper as [117]
SFC separation of ecdysteroids provides shorter retention times, sharper peaks and greater sensitivity than HPLC.
“SFC separation of ecdysteroids, using critical CO 2 , which is non-polar, modified with methanol and silica/bonded (NP) silica HPLC columns or fused silica capillary columns, provides shorter retention times, sharper peaks and greater sensitivity than HPLC.”
From Ecdysteroids (2021) · cites this paper as [117]
Open-column chromatography used to be the standard workhorse for laboratory purification of ecdysteroids but has gradually been replaced by flash chromatography and preparative HPLC.
“Open-column chromatography on silica or alumina eluted with solvents of increasing percentages of methanol or ethanol in hexane, dichloromethane or chloroform used to be the standard 'workhorse' for the laboratory purification of ecdysteroids, as it was of other plant secondary compounds, but this has gradually been replaced by flash chromatography and preparative HPLC on NP-and RP-stationary”
From Ecdysteroids (2021) · cites this paper as [117]
Cites
Li, J., Qi, H., Qi, L., Yi, L., & Li, P. (2007) · Analytica Chimica Acta · doi:10.1016/j.aca.2007.05.016 · Cited by 55 · see citing works
Wilson, I. D., Morgan, E. D., Lafont, R., Shockcor, J. P., Lindon, J. C., Nicholson, J. K., & Wright, B. (1999) · Chromatographia · doi:10.1007/bf02467609 · Cited by 52 · see citing works
Self-citationChromatographic procedures for phytoecdysteroids
Lafont, R., Morgan, E. D., & Wilson, I. D. (1994) · Journal of Chromatography A · doi:10.1016/0021-9673(94)85205-7 · Cited by 46 · see citing works
Self-citationStevens, J. F., Reed, R. L., & Morré, J. T. (2008) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf800211k · Cited by 46 · see citing works
Hikiba, J., Ogihara, M. H., Iga, M., Saito, K., Fujimoto, Y., Suzuki, M., & Kataoka, H. (2012) · Journal of Chromatography B · doi:10.1016/j.jchromb.2012.12.014 · Cited by 44 · see citing works
Modlin, R. F., Alred, P. A., & Tjerneld, F. (1994) · Journal of Chromatography A · doi:10.1016/0021-9673(94)80112-6 · Cited by 34 · see citing works
Girard derivatization for LC-MS/MS profiling of endogenous ecdysteroids in Drosophila
Lavrynenko, O., Nedielkov, R., Möller, H. M., & Shevchenko, A. (2013) · Journal of Lipid Research · doi:10.1194/jlr.d035949 · Cited by 33 · see citing works
High-performance liquid chromatography coupled to nuclear magnetic resonance spectroscopy
Wilson, I. D., Morgan, E. D., Lafont, R., & Wright, B. (1998) · Journal of Chromatography A · doi:10.1016/s0021-9673(97)01299-5 · Cited by 32 · see citing works
Self-citationMiyashita, M., Matsushita, K., Nakamura, S., Akahane, S., Nakagawa, Y., & Miyagawa, H. (2011) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2011.09.002 · Cited by 30 · see citing works
Blais, C., Blasco, T., Maria, A., Dauphin‐Villemant, C., & Lafont, R. (2010) · Journal of Chromatography B · doi:10.1016/j.jchromb.2010.02.018 · Cited by 30 · see citing works
Self-citationRaynor, M. W., Kithinji, J. P., Bartle, K. D., Games, D. E., Mylchreest, I. C., Lafont, R., Morgan, E. D., & Wilson, I. D. (1989) · Journal of Chromatography A · doi:10.1016/s0021-9673(01)93976-7 · Cited by 30 · see citing works
Self-citationSelectivity in the high-performance liquid chromatography of ecdysteroids
Lafont, R., Kaouadji, N., Morgan, E. D., & Wilson, I. D. (1994) · Journal of Chromatography A · doi:10.1016/0021-9673(94)85206-5 · Cited by 28 · see citing works
Self-citationSovová, H., Opletal, L., Sajfrtová, M., & Bártlová, M. (2008) · Journal of Separation Science · doi:10.1002/jssc.200700496 · Cited by 21 · see citing works
Xu, H., Shi, X., Ji, X., Du, Y., Zhu, H. M., & Zhang, L. (2011) · Journal of Separation Science · doi:10.1002/jssc.201000660 · Cited by 20 · see citing works
Destrez, B., Dervilly, G., Bichon, E., Monteau, F., Lafont, R., & Bizec, B. L. (2008) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.3816 · Cited by 20 · see citing works
Self-citationSupercritical fluid extraction of ecdysterone from the roots of <i>Achyranthes bidentata</i> BL.
Zheng, Y., Liu, B., Chen, M., & Chen, T. (2008) · Journal of Separation Science · doi:10.1002/jssc.200700468 · Cited by 19 · see citing works
Li, J., Li, H., Li, P., & Qi, H. (2007) · Biomedical Chromatography · doi:10.1002/bmc.824 · Cited by 19 · see citing works
Preparative-Scale Chromatography of Ecdysteroids: A Class of Biologically Active Steroids
Toth, N., & Báthori, M. (2008) · Journal of Chromatographic Science · doi:10.1093/chromsci/46.2.111 · Cited by 14 · see citing works
Wilson, I. D., & Lafont, R. (1986) · Insect Biochemistry · doi:10.1016/0020-1790(86)90075-2 · Cited by 11 · see citing works
Self-citationIsolation of acidic and conjugated ecdysteroid fractions from Manduca sexta pupae
Lozano, R., Thompson, M. J., Svoboda, J. A., & Lusby, W. R. (1988) · Insect Biochemistry · doi:10.1016/0020-1790(88)90020-0 · Cited by 8 · see citing works
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Lafont, R., Balducci, C., & Dinan, L. (2021) · Encyclopedia · doi:10.3390/encyclopedia1040096 · Cited by 49 · see citing works
Self-citation
- Authors
- Lafont, R., Blais, C., Harmatha, J., & Wilson, I. D.
- Affiliations
- Université Pierre-et-Marie-Curie (FR)
- Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry (CZ)
- Imperial College London (GB)
- Venue
- Reference Module in Chemistry, Molecular Sciences and Chemical Engineering
- Publisher
- Elsevier
- Published
- 2013
- OpenAlex
- W4235622803
- Cited by
- 1
- References
- 20
- Citations by year
- 2021: 1
- FWCI
- 0.00
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Elsevier eBooks (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
- Keywords
- ecdysteroids, polyhydroxylated steroids, thin-layer chromatography, HPLC, normal-phase chromatography, reversed-phase liquid chromatography, diode array detection, mass spectrometry, nanoLC-MS/MS, insect extracts, Drosophila, trace quantification
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