The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Direct transesterification of all classes of lipids in a one-step reaction.
Open accessAbstract
Conventional techniques for the determination of fatty acid composition of lipids require solvent extraction, purification, hydrolysis, and derivatization procedures that are both lengthy and cumbersome. A 1-hr direct transesterification procedure carried out in methanol-benzene 4:1 with acetyl chloride circumvented all these steps and was applicable for analysis of both simple (triglycerides) and complex lipids (cholesteryl esters, phospholipids, and sphingomyelin). Recoveries (greater than 95%) of standards unaffected by the presence of 5% water and 200 mg of silica suggested that the technique could be used for the quantitative analysis of total fatty acids as well as of fatty acids in classes of lipids separated on silica from biological samples. When compared to the Folch procedure, the technique led to a 20.1% increase in total fatty acids for plasma, 3.9% for feces, 7.4% for bile, and 9.7% for rat liver. We therefore conclude that this one-step direct transesterification procedure is superior to currently used methods, not only because of its simplicity and speed, but also because of its added precision.
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Cites
A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride–methanol
Morrison, W. R., & Smith, L. M. (1964) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)40190-7 · Cited by 5,985
A simple procedure for rapid transmethylation of glycerolipids and cholesteryl esters
Christie, W. W. (1982) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38081-0 · Cited by 1,063
Lepage, G., & Roy, C. C. (1984) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)34457-6 · Cited by 1,021
Self-citationLipid Biochemistry: An Introduction
Gurr, M. I., & James, A. T. (1980) · Springer eBooks · doi:10.1007/978-94-009-5907-1 · Cited by 460
Analysis of lipids and lipoproteins
Perkins, E. G. (1975) · Medical Entomology and Zoology · Cited by 418
Putnam, J., Putnam, S., DeVoe, P., & Barness, L. (1982) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/36.1.106 · Cited by 274
Lillington, J. M., Trafford, D. J. H., & Makin, H. L. J. (1981) · Clinica Chimica Acta · doi:10.1016/0009-8981(81)90425-3 · Cited by 133
Human tissue lipids: occurrence of fatty acid isomers from dietary hydrogenated oils.
Ohlrogge, J. B., Emken, E. A., & Gulley, R. M. (1981) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)37333-8 · Cited by 84
Cited by
Newgard, C. B., An, J., Bain, J. R., Muehlbauer, M. J., Stevens, R. D., Lien, L. F., Haqq, A. M., Shah, S. H., Arlotto, M., Slentz, C. A., et al. (2009) · Cell Metabolism · doi:10.1016/j.cmet.2009.02.002 · Cited by 3,165
Siscovick, D. S. (1995) · JAMA: The Journal of the American Medical Association · doi:10.1001/jama.1995.03530170043030 · Cited by 920
Effect of dietary stearic acid on plasma cholesterol and lipoprotein levels
Bonanome, A., & Grundy, S. M. (1988) · New England Journal of Medicine · doi:10.1056/nejm198805123181905 · Cited by 915
Parletta, N., Zarnowiecki, D., Cho, J., Wilson, A., Bogomolova, S., Villani, A., Itsiopoulos, C., Niyonsenga, T., Blunden, S., Meyer, B., et al. (2019) · Nutritional Neuroscience · doi:10.1080/1028415x.2017.1411320 · Cited by 629
Chemical composition and nutritional evaluation of quinoa (Chenopodium quinoa Willd.)
Kozioł, M. J. (1992) · Journal of Food Composition and Analysis · doi:10.1016/0889-1575(92)90006-6 · Cited by 510
Metadata
- Authors
- Lepage, G., & Roy, C. C.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1986-11
- Volume
- 27
- Issue
- 1
- Pages
- 114-120
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2211467290
- Cited by
- 2,384
- References
- 9
- FWCI
- 6.20
- Type
- journal article
- Language
- en
- Open access
- hybrid
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
