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A simple procedure for rapid transmethylation of glycerolipids and cholesteryl esters
Open accessAbstract
A simple procedure suitable for rapid transmethylation of triacylglycerols, other neutral lipids (including cholesteryl esters), and glycerophospholipids is described. Lipids in diethyl ether solution (50 volumes), in the presence of methyl acetate (1 vol), are reacted with 1 M sodium methoxide in methanol (1 vol) at room temperature. Essentially complete transmethylation can occur within a few minutes with no hydrolysis. Glassware and reagent requirements are minimal and samples are ready for gas-liquid chromatography analysis with very little work-up.
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Research works printed but not linked: 5
- Sheppard, 1975, Esterification of fatty acids for gas-liquid chromatographic analysis, J. Chromatogr. Sci., 13, 448, 10.1093/chromsci/13.9.448 · doi:10.1093/chromsci/13.9.448
- Christopherson, 1969, Preparation of milk fat methyl esters by alcoholysis in an essentially nonalcoholic solution, J. Dairy Sci., 52, 1289, 10.3168/jds.S0022-0302(69)86739-1 · DOI · Paper page
- Iverson, 1977, Butyl ester preparation for gas-liquid chromatographic determination of fatty acids in butter, J. Assoc. Off. Anal. Chem., 60, 284
- Timms, 1978, Artefact peaks in the preparation and gas-liquid chromatographic determination of methyl esters, Aust. J. Dairy Technol., 33, 4
- Mattson, 1962, Synthesis and properties of glycerides, J. Lipid Res., 3, 281, 10.1016/S0022-2275(20)40400-6 · DOI · Paper page
Cited by
Direct transesterification of all classes of lipids in a one-step reaction.
Lepage, G., & Roy, C. C. (1986) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38861-1 · Cited by 2,384
The role of Δ9-desaturase in the production of cis-9, trans-11 CLA
Corl, B. A., Baumgard, L. H., Dwyer, D. A., Griinari, J. M., Phillips, B. S., & Bauman, D. E. (2001) · The Journal of Nutritional Biochemistry · doi:10.1016/s0955-2863(01)00180-2 · Cited by 406
Munukka, E., Rintala, A., Toivonen, R., Nylund, M., Yang, B., Takanen, A., Hänninen, A., Vuopio, J., Huovinen, P., Jalkanen, S., et al. (2017) · The ISME Journal · doi:10.1038/ismej.2017.24 · Cited by 278
Tulenko, T. N., Chen, M., Mason, P. E., & Mason, R. P. (1998) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)33861-x · Cited by 172
Buccioni, A., Pauselli, M., Viti, C., Minieri, S., Pallara, G., Roscini, V., Rapaccini, S., Marinucci, M. T., Lupi, P., Conte, G., et al. (2015) · Journal of Dairy Science · doi:10.3168/jds.2014-8651 · Cited by 164
Metadata
- Authors
- Christie, W. W.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1982-09
- Volume
- 23
- Issue
- 7
- Pages
- 1072-1075
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2137419334
- Cited by
- 1,063
- References
- 0
- FWCI
- 1.97
- Type
- journal article
- Language
- en
- Open access
- hybrid
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- creativecommons.org/licenses/by/4.0/ (vor, 13903 d)
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- Christie, W. W. (1982). A simple procedure for rapid transmethylation of glycerolipids and cholesteryl esters. Journal of Lipid Research. https://doi.org/10.1016/s0022-2275(20)38081-0
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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