The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
A stable, radioactive substrate emulsion for assay of lipoprotein lipase
Open accessAbstract
A method is described for the assay of lipoprotein lipase, using a stable, radioactive substrate emulsion. Fatty acid-labeled trioleoylglycerol was emulsified by homogenization in glycerol with lecithin as detergent. This anhydrous emulsion was stable for at least six weeks. Substrate solutions for enzyme assay were prepared by diluting the emulsion with buffer containing serum and albumin. The fatty acid produced on hydrolysis was isolated in a one-step liquid-liquid partition system. Incubations with extracts of acetone powders from adipose tissue displayed characteristics of lipoprotein lipase activity, i.e., serum dependence and inhibition by NaCl and protamine. The method is rapid (less than 1 hour), sensitive and reproducible, and suitable for routine use.
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Citations
Cites
CLEARING FACTOR, A HEPARIN-ACTIVATED LIPOPROTEIN LIPASE
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A rapid assay for lipoprotein lipase
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Self-citationRegulation of lipoprotein lipase
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Self-citationDetermination of heparin-induced lipoprotein lipase activity in human plasma
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Self-citation
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Cited by
Effect of conjugated linoleic acid on body composition in mice
Park, Y., Albright, K. J., Liu, W., Storkson, J. M., Cook, M. E., & Pariza, M. W. (1997) · Lipids · doi:10.1007/s11745-997-0109-x · Cited by 1,163
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Shimizugawa, T., Ono, M., Shimamura, M., Yoshida, K., Ando, Y., Koishi, R., Ueda, K., Inaba, T., Minekura, H., Kohama, T., et al. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m203215200 · Cited by 339
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Metadata
- Authors
- Nilsson-Ehle, P., & Schotz, M. C.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1976-09
- Volume
- 17
- Issue
- 5
- Pages
- 536-541
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2103933115
- Cited by
- 837
- References
- 11
- FWCI
- 10.64
- Type
- journal article
- Language
- en
- Open access
- hybrid
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- creativecommons.org/licenses/by/4.0/ (vor, 16109 d)
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- Nilsson-Ehle, P., & Schotz, M. C. (1976). A stable, radioactive substrate emulsion for assay of lipoprotein lipase. Journal of Lipid Research. https://doi.org/10.1016/s0022-2275(20)41752-3
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
