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Restriction isotyping of human apolipoprotein E by gene amplification and cleavage with HhaI.
Open accessAbstract
We have used restriction isotyping (restriction enzyme isoform genotyping) for rapid typing of common apolipoprotein E isoforms (E2, E3, E4). ApoE restriction isotyping used oligonucleotides to amplify apolipoprotein E gene sequences containing amino acid positions 112 and 158. The amplification products were digested with HhaI and subjected to electrophoresis on polyacrylamide gels. Each of the isoforms was distinguished by a unique combination of HhaI fragment sizes that enabled unambiguous typing of all homozygotic and heterozygotic combinations. HhaI cleaves at GCGC encoding 112arg (E4) and 158arg (E3, E4), but does not cut at GTGC encoding 112cys (E2, E3) and 158cys (E2).
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“The common APOE genotypes can be easily determined by isotyping with the restriction enzyme Hha I13).”
From Impact of Apolipoprotein E Variants: A Review of Naturally Occurring Variants and Clinical Features (2025) · cites this paper as [13]
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Cites
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
Apolipoprotein E polymorphism and atherosclerosis.
Davignon, J., Gregg, R. E., & Sing, C. F. (1988) · Arteriosclerosis: An Official Journal of the American Heart Association, Inc. · doi:10.1161/01.atv.8.1.1 · Cited by 1,981
Zannis, V. I., & Breslow, J. L. (1981) · Biochemistry · doi:10.1021/bi00507a059 · Cited by 563
Research works printed but not linked: 16
- Mahley, 1988, Apolipoprotein E: cholesterol transport protein with expanding role in cell biology, Science., 240, 622, 10.1126/science.3283935 · DOI · Paper page
- Paik, 1989, Nucleotide sequence and structure of the human apolipoprotein E gene, Proc. Natl. Acad. Sci. USA., 82, 3445, 10.1073/pnas.82.10.3445 · DOI · Paper page
- Das, 1985, Isolation, characterization, and mapping to chromosome 19 of the human apolipoprotein E gene, J. Biol. Chem., 260, 6240, 10.1016/S0021-9258(18)88963-3 · DOI
- Myklebost, 1988, A physical map of the apolipoprotein gene cluster on human chromosome 19, Hum. Genet., 78, 244, 10.1007/BF00291670 · DOI
- Utermann, 1980, Genetics of the apolipoprotein E system in man, Am. J. Hum. Genet., 32, 339
- Weisgraber, 1981, Human E apoprotein heterogeneity: cysteine-arginine interchanges in the ami no acid sequence of the apoE isoforms, J. Biol. Chem., 256, 9077, 10.1016/S0021-9258(19)52510-8 · DOI · Paper page
- Boerwinkle, 1989, Am. J. Med. Genet., 27, 567, 10.1002/ajmg.1320270310 · DOI
- Utermann, 1984, Apolipoprotein E phenotypes in patients with myocardial infarction, Hum. Genet., 65, 237, 10.1007/BF00286509 · DOI
- Breslow, 1982, Studies of familial type III hyperlipoproteinemia using as a genetic marker the apoE phenotype E2/2, J. Lipid Res., 23, 1224, 10.1016/S0022-2275(20)38060-3 · DOI · Paper page
- Weisgraber, 1982, Abnormal lipoprotein receptor-binding activity of the human apoE apoprotein due to cysteine-arginine interchange at a single site, J. Biol. Chem., 257, 2518, 10.1016/S0021-9258(18)34954-8 · DOI · Paper page
- Funke, 1986, Detection of apolipoprotein E variants by an oligonucleotide “melting” procedure, Clin. Chem., 32, 1285, 10.1093/clinchem/32.7.1285 · DOI
- Smeets, 1988, Tight linkage between myotonic dystrophy and apolipoprotein E genes revealed with allele-specific oligonucleotides, Hum. Genet., 80, 49, 10.1007/BF00451455 · DOI
- Emi, 1988, Genotyping and sequence analysis of apolipoprotein E isoforms, Genomics., 3, 373, 10.1016/0888-7543(88)90130-9 · DOI · Paper page
- Wallis, 1983, The isolation of cDNA clones for human apolipoprotein E and the detection of apoE RNA in hepatic and extra-hepatic tissues, EMBO J., 2, 2369, 10.1002/j.1460-2075.1983.tb01748.x · DOI
- Hixson, 1988, The baboon gene for apolipoprotein A-I: characterization of a cDNA clone and identification of DNA polymorphisms for genetic studies of cholesterol metabolism, Gene., 74, 483, 10.1016/0378-1119(88)90181-3 · DOI
- Landegren, 1988, DNA diagnostics — molecular techniques and automation, Science., 242, 229, 10.1126/science.3051381 · DOI
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Strittmatter, W. J., Saunders, A. M., Schmechel, D., Pericak-Vance, M., Enghild, J., Salvesen, G. S., & Roses, A. D. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.5.1977 · Cited by 4,268
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Saunders, A. M., Strittmatter, W. J., Schmechel, D., St. George-Hyslop, P. H., Pericak-Vance, M. A., Joo, S. H., Rosi, B. L., Gusella, J. F., Crapper-MacLachlan, D. R., Alberts, M. J., et al. (1993) · Neurology · doi:10.1212/wnl.43.8.1467 · Cited by 3,786
Naj, A. C., Jun, G., Beecham, G. W., Wang, L.-S., Vardarajan, B. N., Buros, J., Gallins, P. J., Buxbaum, J. D., Jarvik, G. P., Crane, P. K., et al. (2011) · Nature Genetics · doi:10.1038/ng.801 · Cited by 1,901
Oxidative Damage Is the Earliest Event in Alzheimer Disease
Nunomura, A., Perry, G., Aliev, G., Hirai, K., Takeda, A., Balraj, E. K., Jones, P. K., Ghanbari, H., Wataya, T., Shimohama, S., et al. (2001) · Journal of Neuropathology & Experimental Neurology · doi:10.1093/jnen/60.8.759 · Cited by 1,885
Lautenschlager, N. T., Cox, K. L., Flicker, L., Foster, J. K., van Bockxmeer, F. M., Xiao, J., Greenop, K. R., & Almeida, O. P. (2008) · JAMA · doi:10.1001/jama.300.9.1027 · Cited by 1,637
Metadata
- Authors
- Hixson, J., & Vernier, D.
- Affiliations
- Texas Biomedical Research Institute (US)
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1990-03
- Volume
- 31
- Issue
- 3
- Pages
- 545-548
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2111774300
- Cited by
- 2,933
- References
- 3
- Citations by year
- 2026: 19 · 2025: 36 · 2024: 40 · 2023: 35 · 2022: 43 · 2021: 48 · 2020: 48 · 2019: 48 · 2018: 56 · 2017: 54 · 2016: 59 · 2015: 76 · 2014: 82 · 2013: 93 · 2012: 89
- FWCI
- 33.40
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Lipid Research (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Heart, Lung, and Blood Institute (HL-28972, P01HL028972)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipoproteins and Cardiovascular Health
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Associations and Epidemiology
- Keywords
- apolipoprotein E, HhaI, gene amplification, apolipoprotein E isoforms
- MeSH terms
- Amino Acid Sequence, Apolipoproteins E, Base Sequence, DNA, Gene Amplification, Humans, Leukocytes, Molecular Sequence Data, Polymorphism, Genetic, Deoxyribonucleases, Type II Site-Specific, Polymerase Chain Reaction
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