Bioelectrorheological model of the cell. 2. Analysis of creep and its experimental verification
Abstract
The electrorheological model of the cell proposed in Part 1 of this work was used to analyze changes in time of the shape of a cell acted on by a constant-amplitude external alternating electric field, with lossiness of the media taken into account. Shear stress in the cell membrane was determined. This model was then subjected to preliminary experimental verification using Neurospora crassa (slime) spheroplasts subjected to an external alternating electric field of constant frequency (3 MHz) and varying magnitude for different periods of time. Reversible viscoelastic cell deformation and fatigue (stiffening) of the material were observed. A satisfactory fit of the experimental data to Burgers' rheological model was found, and the values of the elastic moduli E1 = 9 X 10(2) N/m2, E2 = 2 X 10(2) N/m2 and viscosities eta 1 = 8 X 10(4) Ns/m2; eta 2 = 7 X 10(3) Ns/m2 were evaluated.
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In N. crassa, the bioelectrorheological model predicted changes in cell volume and surface caused by AEFs of 3000 kHz.
“Further testing in N. crassa (mold cells) showed that the model was able to predict changes in cell volume and surface caused by AEFs of 3000 kHz [54].”
From Permeabilizing Cell Membranes with Electric Fields (2021) · cites this paper as [54]
The bioelectrorheological model demonstrates the relationship between AEFs, shape deformations, and membrane destabilization, which can contribute to electroporation and other electric field-induced cell phenomena including electrofusion and electro-destruction.
“The bioelectrorheological model of the cell was proposed and has been expanded upon through a series of manuscripts by Pawlowski et al. [40,53,54,55,56,57] (Figure 3B). This model demonstrates the relationship between AEFs, shape deformations, and membrane destabilization, which can contribute to electroporation and other electric field-induced cell phenomena including electrofusion and electro-d”
From Permeabilizing Cell Membranes with Electric Fields (2021) · cites this paper as [54]
The bioelectrorheological model provides a possible explanation for the causes of electroporation by demonstrating the combined effects of heat energy and extensile stress leading to reversible cell membrane destabilization, which can directly result in electroporation.
“Although the electric field strengths studied by Pawlowski and colleagues [40,53,54,55,56,57] were two orders of magnitude greater than that of TTFields, the bioelectrorheological model provides a possible explanation to the causes of electroporation by demonstrating the combined effects of heat energy and extensile stress leading to reversible cell membrane destabilization, which can directly”
From Permeabilizing Cell Membranes with Electric Fields (2021) · cites this paper as [54]
Both DC and AC electroporation are believed to cause a dielectric breakdown of the cell; the oscillating nature of AC electroporation is thought to provoke structural fatigue of the membrane, comparable to the extensile stress proposed in the bioelectrorheological model.
“Both types of electroporation are believed to cause a dielectric breakdown of the cell. However, the oscillating nature of AC electroporation is thought to provoke structural fatigue of the membrane [61], comparable to the extensile stress proposed in the bioelectrorheological model [40,53,54,55,56,57].”
From Permeabilizing Cell Membranes with Electric Fields (2021) · cites this paper as [54]
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Permeabilizing Cell Membranes with Electric Fields
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Bioelectrorheological model of the cell. 3. Viscoelastic shear deformation of the membrane
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- Authors
- Fikus, M., & Pawlowski, P.
- Affiliations
- Institute of Biochemistry and Biophysics, Polish Academy of Sciences (PL)
- Venue
- Journal of Theoretical Biology
- Publisher
- Elsevier BV
- Published
- 1989-04
- Volume
- 137
- Issue
- 4
- Pages
- 365-373
- ISSN
- 0022-5193 (ISSN-L)
- OpenAlex
- W2061373740
- Cited by
- 11
- References
- 10
- Citations by year
- 2024: 1 · 2023: 1 · 2021: 1 · 2018: 1 · 2014: 1 · 2012: 1
- FWCI
- 0.15
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Theoretical Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Electromagnetic Fields and Biological Effects
- Physical Sciences › Chemical Engineering › Fluid Flow and Transfer Processes › Rheology and Fluid Dynamics Studies
- Keywords
- viscoelasticity, AC electric field, Burgers model, elastic modulus, viscosity, cell deformation, lossy media
- MeSH terms
- Cell Membrane, Mathematics, Models, Biological, Neurospora crassa, Rheology, Stress, Mechanical
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