Caulerpenyne from Caulerpa taxifolia has an antiproliferative activity on tumor cell line SK-N-SH and modifies the microtubule network
Abstract
Caulerpenyne, the major secondary metabolite synthesized by the green marine alga Caulerpa taxifolia, is cytotoxic against several cell lines. To identify possible targets of this toxin, we investigated the effect of caulerpenyne on the neuroblastoma SK-N-SH cell line. Caulerpenyne induced an inhibition of SK-N-SH cell proliferation with an IC50 of 10 +/- 2 microM after 2 hr of incubation. We observed no blockage in G2/M phase and an increase in cell death. On immunofluorescence microscopy, caulerpenyne affected the microtubule network in SK-N-SH cell line; we observed a loss of neurites and a compaction of the microtubule network at the cell periphery. In vitro, after 35 min of incubation, caulerpenyne inhibited the polymerization of pig brain purified tubulin or microtubule proteins, with an IC50 of 21 +/- 2 microM and 51 +/- 6 microM respectively. Analysis by electron microscopy indicated that caulerpenyne induced aggregation of tubulin, which may be responsible for inhibition of microtubule polymerization and bundling of residual microtubules.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · see citing works
Mosmann, T. (1983) · Journal of Immunological Methods · doi:10.1016/0022-1759(83)90303-4 · Cited by 56,104 · see citing works
Taxol: an antimitotic agent with a new mechanism of action
Manfredi, J. J., & Horwitz, S. B. (1984) · Pharmacology & Therapeutics · doi:10.1016/0163-7258(84)90025-1 · Cited by 486 · see citing works
Gerwick, W. H., Proteau, P. J., Nagle, D. G., Hamel, E., Blokhin, A., & Slate, D. L. (1994) · The Journal of Organic Chemistry · doi:10.1021/jo00085a006 · Cited by 365 · see citing works
Pharmaceuticals from cultured algae
Schwartz, R. E., Hirsch, C. F., Sesin, D. F., Flor, J. E., Chartrain, M. M., Fromtling, R. E., Harris, G. H., Salvatore, M. J., Liesch, J. M., & Yudin, K. (1990) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/bf01573860 · Cited by 315 · see citing works
Inhibition of tubulin-microtubule polymerization by drugs of the Vinca alkaloid class.
Owellen, R. J., Hartke, C., Dickerson, R. M., & Hains, F. O. (1976) · PubMed · Cited by 207 · see citing works
Bundling of microtubules by glyceraldehyde‐3‐phosphate dehydrogenase and its modulation by ATP
Huitorel, P., & Pantaloni, D. (1985) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1985.tb09016.x · Cited by 181 · see citing works
Self-citationHernández, M., Burillo, S., Crespo, M. S., & Nieto, M. L. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.1.606 · Cited by 156 · see citing works
Loike, J. D., Brewer, C. F., Sternlicht, H., et al. (1978) · PubMed · Cited by 143 · see citing works
Chemical defence of the mediterranean alga Caulerpa taxifolia: variations in caulerpenyne production
Amade, P., & Lemée, R. (1998) · Aquatic Toxicology · doi:10.1016/s0166-445x(98)00054-x · Cited by 108 · see citing works
Self-citationIdentification of a distinct class of vinblastine binding sites on microtubules
Jordan, M. A., Margolis, R. L., Himes, R. H., et al. (1986) · Journal of Molecular Biology · doi:10.1016/0022-2836(86)90406-7 · Cited by 104 · see citing works
Fischel, J.-L., Rodolphe Lemée, B., Formento, P., et al. (1996) · PubMed · Cited by 82 · see citing works
Differentiation of human colon cancer cells changes the expression of β-tubulin isotypes and MAPs
Carles, G., Braguer, D., Dumontet, C., et al. (1999) · British Journal of Cancer · doi:10.1038/sj.bjc.6690481 · Cited by 61 · see citing works
Comparative effects of taxol and Taxotere on two different human carcinoma cell lines
Garcia, P., Braguer, D., Carles, G., et al. (1994) · Cancer Chemotherapy and Pharmacology · doi:10.1007/bf00686042 · Cited by 57 · see citing works
SHORT COMMUNICATION. Antimicrobial and Antineoplastic Activity in Some South Florida Seaweeds
Hodgson, L. M. (1984) · Botanica Marina · doi:10.1515/botm.1984.27.8.387 · Cited by 56 · see citing works
Structure and Function in the Tubulin Dimer and the Role of the Acidic Carboxyl Terminus
Sackett, D. L. (1995) · Sub-cellular biochemistry/Subcellular biochemistry · doi:10.1007/978-1-4899-1727-0_9 · Cited by 53 · see citing works
Phospholipase A2 inhibitors from marine algae
Mayer, A. M. S., Paul, V. J., Fenical, W., Norris, J. N., de Carvalho, M. S., & Jacobs, R. S. (1993) · Springer eBooks · doi:10.1007/978-94-011-1998-6_69 · Cited by 51 · see citing works
Parent-Massin, D. (1996) · Journal of Toxicology and Environmental Health · doi:10.1080/009841096161924 · Cited by 40 · see citing works
C-terminal cleavage of tubulin by subtilisin enhances ring formation
Peyrot, V., Briand, C., & Andreu, J. M. (1990) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(90)90499-o · Cited by 25 · see citing works
Self-citationAction of hydrolyzed cisplatin and some analogs on microtubule protein polymerization in vitro.
Peyrot, V., Briand, C., Crevat, A. D., et al. (1983) · PubMed · Cited by 25 · see citing works
Self-citationBarbier, P., Peyrot, V., Leynadier, D., et al. (1998) · Biochemistry · doi:10.1021/bi970568t · Cited by 23 · see citing works
Self-citationLi, Y., Kobayashi, H., Hashimoto, Y., et al. (1994) · Chemico-Biological Interactions · doi:10.1016/0009-2797(94)90018-3 · Cited by 21 · see citing works
Caulerpenyne blocks MBP kinase activation controlling mitosis in sea urchin eggs
Pesando, D., Pesci-Bardona, C., Huitorel, P., et al. (1999) · European Journal of Cell Biology · doi:10.1016/s0171-9335(99)80092-2 · Cited by 14 · see citing works
Self-citationThe disruption of brain microtubules in vitro by the phospholipase inhibitor p-bromophenacyl bromide
Hargreaves, A. J., Glazier, A. P., Flaskos, J., et al. (1994) · Biochemical Pharmacology · doi:10.1016/0006-2952(94)90384-0 · Cited by 8 · see citing works
Berman, F. W., & Murray, T. F. (1996) · Journal of Biochemical and Molecular Toxicology · doi:10.1002/(sici)1522-7146(1996)11:5 · Cited by 2 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Medicinal and pharmaceutical uses of seaweed natural products: A review
Smit, A. J. (2004) · Journal of Applied Phycology · doi:10.1023/b:japh.0000047783.36600.ef · Cited by 806 · see citing works
Marine Natural Products: A Source of Novel Anticancer Drugs
Khalifa, S. A. M., Elias, N., Farag, M. A., Chen, L., Saeed, A., Hegazy, M.-E. F., Moustafa, M. S., Abd El-Wahed, A., Al-Mousawi, S. M., Musharraf, S. G., et al. (2019) · Marine Drugs · doi:10.3390/md17090491 · Cited by 555 · see citing works
“algea attributed to anticancer and antiproliferative effects against neuroblastoma cell line through induction of cells inhibition proliferation with an IC50 of 10 μM [79].”
Background
- Authors
- Barbier, P., Guise, S., Huitorel, P., Amade, P., Pesando, D., Briand, C., & Peyrot, V.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Université Nice Sophia Antipolis (FR)
- Corresponding author
- Barbier, P.
- ORCID
- Barbier, P.
- Venue
- Life Sciences
- Publisher
- Elsevier BV
- Published
- 2001-12
- Volume
- 70
- Issue
- 4
- Pages
- 415-429
- ISSN
- 0024-3205 (ISSN-L)
- OpenAlex
- W2084182951
- Cited by
- 110
- References
- 25
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 9 · 2022: 3 · 2021: 4 · 2020: 9 · 2019: 9 · 2018: 6 · 2017: 6 · 2016: 4 · 2015: 1 · 2014: 5 · 2013: 7 · 2012: 4
- FWCI
- 0.57
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Life Sciences (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Health Sciences › Medicine › Oncology › Cancer Treatment and Pharmacology
- Keywords
- caulerpenyne, Caulerpa taxifolia, marine algal toxins, antiproliferative activity, microtubule cytoskeleton, tubulin polymerization inhibition, cytotoxicity, cell death, secondary metabolites
- MeSH terms
- Antineoplastic Agents, Cell Division, Cell Survival, Dose-Response Relationship, Drug, Flow Cytometry, Fluorescent Antibody Technique, Humans, Microtubules, Neuroblastoma, Plant Extracts, Sesquiterpenes, Tetrazolium Salts, Tumor Cells, Cultured
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review