Cited by 110Microtubule and mitosis dynamicsSeaweed-derived Bioactive CompoundsCancer Treatment and Pharmacology

Caulerpenyne from Caulerpa taxifolia has an antiproliferative activity on tumor cell line SK-N-SH and modifies the microtubule network

Barbier, P., Guise, S., Huitorel, P., Amade, P., Pesando, D., Briand, C., Peyrot, V.·2001·Life Sciences

Abstract

Caulerpenyne, the major secondary metabolite synthesized by the green marine alga Caulerpa taxifolia, is cytotoxic against several cell lines. To identify possible targets of this toxin, we investigated the effect of caulerpenyne on the neuroblastoma SK-N-SH cell line. Caulerpenyne induced an inhibition of SK-N-SH cell proliferation with an IC50 of 10 +/- 2 microM after 2 hr of incubation. We observed no blockage in G2/M phase and an increase in cell death. On immunofluorescence microscopy, caulerpenyne affected the microtubule network in SK-N-SH cell line; we observed a loss of neurites and a compaction of the microtubule network at the cell periphery. In vitro, after 35 min of incubation, caulerpenyne inhibited the polymerization of pig brain purified tubulin or microtubule proteins, with an IC50 of 21 +/- 2 microM and 51 +/- 6 microM respectively. Analysis by electron microscopy indicated that caulerpenyne induced aggregation of tubulin, which may be responsible for inhibition of microtubule polymerization and bundling of residual microtubules.

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Crossref · 10.1016/s0024-3205(01)01396-0

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Authors
Barbier, P., Guise, S., Huitorel, P., Amade, P., Pesando, D., Briand, C., & Peyrot, V.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • Université Nice Sophia Antipolis (FR)
Corresponding author
Barbier, P.
Venue
Life Sciences
Publisher
Elsevier BV
Published
2001-12
Volume
70
Issue
4
Pages
415-429
ISSN
0024-3205 (ISSN-L)
OpenAlex
W2084182951
Cited by
110
References
25
Citations by year
2026: 3 · 2025: 2 · 2024: 9 · 2022: 3 · 2021: 4 · 2020: 9 · 2019: 9 · 2018: 6 · 2017: 6 · 2016: 4 · 2015: 1 · 2014: 5 · 2013: 7 · 2012: 4
FWCI
0.57
Type
journal article
Language
en
Open access
closed
Locations
  • Life Sciences (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
  • Health Sciences › Medicine › Oncology › Cancer Treatment and Pharmacology
Keywords
caulerpenyne, Caulerpa taxifolia, marine algal toxins, antiproliferative activity, microtubule cytoskeleton, tubulin polymerization inhibition, cytotoxicity, cell death, secondary metabolites
MeSH terms
Antineoplastic Agents, Cell Division, Cell Survival, Dose-Response Relationship, Drug, Flow Cytometry, Fluorescent Antibody Technique, Humans, Microtubules, Neuroblastoma, Plant Extracts, Sesquiterpenes, Tetrazolium Salts, Tumor Cells, Cultured

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