Evaluation of the toxicological risk to humans of caulerpenyne using human hematopoietic progenitors, melanocytes, and keratinocytes in culture
Abstract
The extensive growth of Caulerpa taxifolia in the Mediterranean sea produces important quantities of bioactive secondary metabolites unable to enter the food chain. The cytotoxic effects of caulerpenyne, the major secondary metabolite from C. taxifolia, was studied in different in vitro models: skin cells, primary cultures of melanocytes and keratinocytes, immortalized keratinocytes (HaCaT and HESV), and bone marrow cells (hematopoietic progenitors CFU-GM). Typical dose-response curves from neutral red uptake and MTT assays were recorded in all models with IC50 ranging from 6 to 24 microM. Hematopoietic progenitors were more sensitive to caulerpenyne than melanocyte and keratinocyte cell lines, which could be due to their higher proliferative rate. The distribution of aggregates in colonies, macroclusters, and microclusters of hematopoietic progenitors was also altered in the presence of caulerpenyne. From our evaluation of the caulerpenyne concentrations required to result in cellular toxicity, the risks of cutaneous and/or food intoxication to humans may be considered minimal.
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- Authors
- Parent-Massin, D.
- Venue
- Journal of Toxicology and Environmental Health
- Publisher
- Informa UK Limited
- Published
- 1996-01
- Volume
- 47
- Issue
- 1
- Pages
- 47-59
- ISSN
- 0098-4108 (ISSN-L)
- OpenAlex
- W2014087980
- Cited by
- 40
- References
- 0
- Citations by year
- 2024: 4 · 2023: 1 · 2021: 3 · 2020: 4 · 2019: 2 · 2017: 1 · 2016: 1 · 2013: 1 · 2012: 1
- FWCI
- 0.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Toxicology and Environmental Health (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Physical Sciences › Environmental Science › Environmental Chemistry › Marine Toxins and Detection Methods
- Keywords
- caulerpenyne, Caulerpa taxifolia, cytotoxicity, hematopoietic progenitor cells, CFU-GM, melanocytes, keratinocytes, HaCaT keratinocytes, secondary metabolites, dose-response relationship, cell aggregation, skin toxicity, marine invasive species
- MeSH terms
- Chlorophyta, Cells, Cultured, Chromatography, High Pressure Liquid, Dose-Response Relationship, Drug, Coloring Agents, Fetal Blood, Hematopoietic Stem Cells, Humans, Lethal Dose 50, Marine Toxins, Melanocytes, Neutral Red, Sesquiterpenes, Tetrazolium Salts, Thiazoles, Keratinocytes, Eukaryota
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