The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
N-methyl-D-aspartate receptors: different subunit requirements for binding of glutamate antagonists, glycine antagonists, and channel-blocking agents
Abstract
Expression of the NR-1 subunit in Xenopus oocytes produces channels that respond to glutamate and are blocked by competitive and noncompetitive antagonists of the N-methyl-D-aspartate (NMDA) receptor. Ionic conductances through these channels are increased by coexpression with NR-2 receptor subunits. We have characterized the pharmacological properties of NMDA receptors assembled from combinations of subunits expressed in transfected cells, to determine the minimum subunit requirements for binding of competitive glutamate antagonists, glycine antagonists, and channel-blocking agents, as detected by ligand-binding experiments. Expression of NR-1a alone produced glycine antagonist binding, whereas the combination of NR-1a and NR-2A was needed to produce binding sites for glutamate antagonists and channel-blocking agents. These results suggest that functional NMDA receptors assemble from these subunits. However, differences in the pharmacological effects of NMDA and polyamines show that not all characteristics of native NMDA receptors are reproduced by this combination of subunits.
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“NMDA receptors are ligand-gated cation channels with crucial roles in synaptic transmission and plasticity.”
From Anti-NMDA-receptor encephalitis: case series and analysis of the effects of antibodies (2008) · cites this paper as [1]
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“The receptors are heteromers of NR1 subunits that bind glycine and NR2 (A, B, C, or D) subunits that bind glutamate.1”
From Anti-NMDA-receptor encephalitis: case series and analysis of the effects of antibodies (2008) · cites this paper as [1]
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PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 316,394
Yung-Chi, C., & Prusoff, W. H. (1973) · Biochemical Pharmacology · doi:10.1016/0006-2952(73)90196-2 · Cited by 12,922
High-Efficiency Transformation of Mammalian Cells by Plasmid DNA
Chen, C., & Okayama, H. (1987) · Molecular and Cellular Biology · doi:10.1128/mcb.7.8.2745 · Cited by 5,327
Molecular cloning and characterization of the rat NMDA receptor
Moriyoshi, K., Masu, M., Ishii, T., Shigemoto, R., Mizuno, N., & Nakanishi, S. (1991) · Nature · doi:10.1038/354031a0 · Cited by 1,771
Calcium-mediated neurotoxicity: relationship to specific channel types and role in ischemic damage
Choi, D. W. (1988) · Trends in Neurosciences · doi:10.1016/0166-2236(88)90200-7 · Cited by 1,726
Flip and Flop: A Cell-Specific Functional Switch in Glutamate-Operated Channels of the CNS
Sommer, B., KEINÄNEN, K. P., Verdoorn, T. A., Wisden, W., Burnashev, N., Herb, A., Köhler, M. G., Takagi, T., Sakmann, B. J., & Seeburg, P. H. (1990) · Science · doi:10.1126/science.1699275 · Cited by 1,272
Williams, K. L. (1993) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)13275-6 · Cited by 860
Functional characterization of a heteromeric NMDA receptor channel expressed from cloned cDNAs
Meguro, H., Mori, H., Araki, K., Kushiya, E., Kutsuwada, T., Yamazaki, M., Kumanishi, T., Arakawa, M., Sakimura, K., & Mishina, M. (1992) · Nature · doi:10.1038/357070a0 · Cited by 799
Zinc potentiates agonist-lnduced currents at certain splice variants of the NMDA receptor
Hollmann, M., Boulter, J., Maron, C., Beasley, L., Sullivan, J., Pecht, G., & Heinemann, S. F. (1993) · Neuron · doi:10.1016/0896-6273(93)90209-a · Cited by 614
[3H]MK‐801 Labels a Site on the N‐Methyl‐D‐Aspartate Receptor Channel Complex in Rat Brain Membranes
Wong, E. H. F., Knight, A. R., & Woodruff, G. N. (1988) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1988.tb13260.x · Cited by 454
Reynolds, I. J., Murphy, S. N., & Miller, R. J. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.21.7744 · Cited by 336
Blount, P., & Merlie, J. P. (1989) · Neuron · doi:10.1016/0896-6273(89)90259-6 · Cited by 285
Stern, P., Béhé, P., Schoepfer, R., & Colquhoun, D. (1992) · Proceedings of the Royal Society B Biological Sciences · doi:10.1098/rspb.1992.0159 · Cited by 267
Sills, M. A., Fagg, G. E., Pozza, M. F., Angst, C., Brundish, D. E., Hurt, S. D., Wilusz, E. J., & Williams, M. J. (1991) · European Journal of Pharmacology · doi:10.1016/0014-2999(91)90063-v · Cited by 211
Rock, D. M., & Macdonald, R. L. (1992) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)08850-9 · Cited by 124
Polyamines regulate glycine interaction with the N‐methyl‐ D ‐aspartate receptor
Ransom, R. W., & Deschenes, N. L. (1990) · Synapse · doi:10.1002/syn.890050406 · Cited by 78
Chazot, P. L., Cik, M., & Stephenson, F. A. (1992) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1992.tb08364.x · Cited by 70
Developmental Changes in the Sensitivity of the N-Methyl-d-Aspartate Receptor to Polyamines
Williams, K. L., Hanna, J. L., & Molinoff, P. B. (1991) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)15397-2 · Cited by 56
Molecular target size analyses of the NMDA-receptor complex in rat cortex
Honoré, T., Drejer, J., Nielsen, E. Ø., Watkins, J. C., Olverman, H. J., & Nielsen, M. (1989) · European Journal of Pharmacology Molecular Pharmacology · doi:10.1016/0922-4106(89)90054-0 · Cited by 50
Research works printed but not linked: 1
- Monaghan, D. T., H. J. Olverman, L. Nguyen, J. C. Watkins, and C. W. Cotman. Two classes of N-methyl-D-aapartate recognition sites: differential distribution and differential regulation by glycine. Proc. NatL Acad. Sci. USA 85:9836-9840 (1988).
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Anti-NMDA-receptor encephalitis: case series and analysis of the effects of antibodies
Dalmau, J., Gleichman, A. J., Hughes, E. G., Rossi, J. E., Peng, X., Lai, M., Dessain, S. K., Rosenfeld, M. R., Balice-Gordon, R., & Lynch, D. R. (2008) · The Lancet Neurology · doi:10.1016/s1474-4422(08)70224-2 · Cited by 3,488
Self-citationParaneoplastic anti– N ‐methyl‐ D ‐aspartate receptor encephalitis associated with ovarian teratoma
Dalmau, J., Tüzün, E., Wu, H., Masjuan, J., Rossi, J. E., Voloschin, A., Baehring, J. M., Shimazaki, H., Koide, R., King, D., et al. (2007) · Annals of Neurology · doi:10.1002/ana.21050 · Cited by 2,492
The Role of Excitotoxicity in Neurodegenerative Disease
Doble, A. (1999) · Pharmacology & Therapeutics · doi:10.1016/s0163-7258(98)00042-4 · Cited by 723
Danysz, W., & Parsons, C. G. (1998) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01382-6 · Cited by 652
Michaelis, E. K. (1998) · Progress in Neurobiology · doi:10.1016/s0301-0082(97)00055-5 · Cited by 578
Metadata
- Authors
- Lynch, D. R., Anegawa, N. J., Verdoorn, T., & Pritchett, D. B.
- Affiliations
- University of Pennsylvania (US)
- Venue
- Molecular Pharmacology
- Publisher
- Elsevier BV
- Published
- 1994-03
- Volume
- 45
- Issue
- 3
- Pages
- 540-545
- ISSN
- 0026-895X (ISSN-L)
- OpenAlex
- W1839333079
- Cited by
- 173
- References
- 19
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 4 · 2018: 3 · 2017: 3 · 2016: 4 · 2015: 4 · 2014: 2 · 2013: 4 · 2012: 1
- FWCI
- 4.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Pharmacology (publishedVersion)
- PubMed (publishedVersion)
- PubMed
- Funders
- National Institutes of Health (5r01da007130-08)
- National Institute on Drug Abuse (DA 07130-02)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Keywords
- NMDA receptor, NR1 subunit, NR2A subunit, subunit assembly, glutamate receptor antagonists, glycine antagonist, ligand binding, polyamines, competitive antagonism, non-competitive antagonism, ion channel pharmacology
- MeSH terms
- Binding Sites, Cells, Cultured, Glutamates, Glycine, Humans, Ion Channels, Kynurenic Acid, Transfection, Receptors, N-Methyl-D-Aspartate, Dizocilpine Maleate, Excitatory Amino Acid Antagonists, Glutamic Acid
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