Molecular cloning and characterization of the rat NMDA receptor
Abstract
A complementary DNA encoding the rat NMDA receptor has been cloned and characterized. The single protein encoded by the cDNA forms a receptor-channel complex that has electrophysiological and pharmacological properties characteristic of the NMDA receptor. This protein has a significant sequence similarity to the AMPA/kainate receptors and contains four putative transmembrane segments following a large extracellular domain. The NMDA receptor messenger RNA is expressed in neuronal cells throughout the brain regions, particularly in the hippocampus, cerebral cortex and cerebellum.
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The NMDAR1 gene encodes the essential subunit for the NMDAR.
“Here we describe an application of this method to the NMDA receptor 1 gene ( NMDAR1 ), which encodes the essential subunit for the NMDAR (Moriyoshi et al., 1991;Nakanishi,1992).”
From The essential role of hippocampal CA1 NMDA receptor-dependent synaptic plasticity in spatial memory. (1996) · cites this paper as Moriyoshi et al., 1991
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- Authors
- Moriyoshi, K., Masu, M., Ishii, T., Shigemoto, R., Mizuno, N., & Nakanishi, S.
- Affiliations
- Kyoto University (JP)
- Venue
- Nature
- Published
- 1991-11-07
- Volume
- 354
- Issue
- 6348
- Pages
- 31-37
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2059334396
- Cited by
- 1,771
- References
- 0
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 7 · 2023: 19 · 2022: 15 · 2021: 12 · 2020: 21 · 2019: 22 · 2018: 22 · 2017: 20 · 2016: 14 · 2015: 26 · 2014: 28 · 2013: 18 · 2012: 27
- FWCI
- 53.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- NMDA receptor, rats, cDNA cloning, transmembrane helices, extracellular domain, mRNA expression, hippocampus, cerebral cortex, cerebellum, neurons
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Brain, Cloning, Molecular, Electrophysiology, Ibotenic Acid, Kainic Acid, Molecular Sequence Data, RNA, Messenger, Xenopus, Molecular Structure, Receptors, N-Methyl-D-Aspartate, alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid, Rats
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