Cited by 155Drug-Induced Adverse ReactionsDrug-Induced Hepatotoxicity and ProtectionGlutathione Transferases and Polymorphisms

Reactions of the nitroso and hydroxylamine metabolites of sulfamethoxazole with reduced glutathione. Implications for idiosyncratic toxicity

Cribb, A E, Miller, M, Leeder, J S, Hill, J, Spielberg, S P·1991·Drug Metabolism and Disposition

Abstract

N4-oxidation of sulfonamides has been implicated in the pathogenesis of idiosyncratic reactions to these antimicrobials. In vitro toxicity assays employing mononuclear leukocytes as target cells have shown that the toxicity of sulfamethoxazole hydroxylamine (SMX-HA) is inhibited by exogenous glutathione, suggesting that conjugation with glutathione is an important detoxification pathway. However, in these experiments, significant depletion of cellular glutathione only occurred at concentrations of SMX-HA greater than or equal to 300 microM. At concentrations of SMX-HA which produce 50% toxicity in mononuclear leukocytes (approximately 100 microM), there was not a significant loss of glutathione. SMX-HA also caused a small but significant increase in oxidized glutathione concentrations. In cell-free experiments, reduced glutathione (GSH) prevented the autooxidation of SMX-HA to nitrososulfamethoxazole (nitroso-SMX). During this process, oxidized glutathione was formed. GSH rapidly reacted with nitroso-SMX to form a labile semimercaptal conjugate. Physiologically relevant concentrations of GSH (i.e. 1 mM) favored thiolytic cleavage of the semimercaptal to form SMX-HA. Isomerization of the semimercaptal to the more stable sulfinamide occurred at low GSH concentrations. Purified glutathione transferases had no effect on the reaction of SMX-HA with GSH. Therefore, glutathione is important in protecting cells from the toxicity of SMX-HA largely by preventing its further oxidation to nitroso-SMX. Stable glutathione conjugates are likely to be formed only in small quantities under physiological conditions. Conjugation with glutathione would not be expected to be a major pathway for clearance of the hydroxylamine and nitroso metabolites of sulfonamides.

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Crossref · 10.1016/s0090-9556(25)08967-6

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Authors
Cribb, A. E., Miller, M., Leeder, J. S., Hill, J., & Spielberg, S. P.
Affiliations
University of Toronto (CA)
Venue
Drug Metabolism and Disposition
Publisher
Elsevier BV
Published
1991-09
Volume
19
Issue
5
Pages
900-906
ISSN
0090-9556 (ISSN-L)
OpenAlex
W1790339004
Cited by
155
References
0
Citations by year
2026: 2 · 2025: 1 · 2024: 2 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 3 · 2018: 7 · 2017: 5 · 2016: 1 · 2015: 2 · 2014: 7 · 2013: 5 · 2012: 7
FWCI
2.94
Type
journal article
Language
en
Open access
closed
Locations
  • Drug Metabolism and Disposition (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed
Topics
  • Health Sciences › Medicine › Pharmacology › Drug-Induced Adverse Reactions
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glutathione Transferases and Polymorphisms
Keywords
sulfamethoxazole, reduced glutathione, glutathione conjugation, idiosyncratic drug toxicity, mononuclear leukocytes, glutathione depletion, glutathione disulfide, glutathione S-transferase, drug detoxification
MeSH terms
Cell-Free System, Glutathione, Glutathione Reductase, Humans, Leukocytes, Mononuclear, Inactivation, Metabolic, Oxidation-Reduction, Sulfamethoxazole, In Vitro Techniques

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