The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Human Upf Proteins Target an mRNA for Nonsense-Mediated Decay When Bound Downstream of a Termination Codon
Open accessAbstract
Nonsense-mediated decay (NMD) rids eukaryotic cells of aberrant mRNAs containing premature termination codons. These are discriminated from true termination codons by downstream cis-elements, such as exon-exon junctions. We describe three novel human proteins involved in NMD, hUpf2, hUpf3a, and hUpf3b. While in HeLa cell extracts these proteins are complexed with hUpf1, in intact cells hUpf3a and hUpf3b are nucleocytoplasmic shuttling proteins, hUpf2 is perinuclear, and hUpf1 cytoplasmic. hUpf3a and hUpf3b associate selectively with spliced beta-globin mRNA in vivo, and tethering of any hUpf protein to the 3'UTR of beta-globin mRNA elicits NMD. These data suggest that assembly of a dynamic hUpf complex initiates in the nucleus at mRNA exon-exon junctions and triggers NMD in the cytoplasm when recognized downstream of a translation termination site.
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Cites
Messenger RNA degradation in eukaryotes
Sachs, A. B. (1993) · Cell · doi:10.1016/0092-8674(93)80043-e · Cited by 846
Binary specification of nonsense codons by splicing and cytoplasmic translation
Thermann, R., Neu‐Yilik, G., Deters, A., Frede, U., Wehr, K., Hagemeier, C., Hentze, M. W., & Kulozik, A. E. (1998) · The EMBO Journal · doi:10.1093/emboj/17.12.3484 · Cited by 406
Cited by
Nonsense-mediated mRNA decay: splicing, translation and mRNP dynamics
Maquat, L. E. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1310 · Cited by 1,214
The cap-to-tail guide to mRNA turnover
Wilusz, C. J., Wormington, M., & Peltz, S. W. (2001) · Nature Reviews Molecular Cell Biology · doi:10.1038/35067025 · Cited by 759
How introns influence and enhance eukaryotic gene expression
Le Hir, H., Nott, A., & Moore, M. J. (2003) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(03)00052-5 · Cited by 734
Boutz, P. L., Stoilov, P., Li, Q., Lin, C.-H., Chawla, G., Ostrow, K., Shiue, L., Ares, M., & Black, D. L. (2007) · Genes & Development · doi:10.1101/gad.1558107 · Cited by 577
Johnson, E. C. B., Carter, E. K., Dammer, E. B., Duong, D. M., Gerasimov, E. S., Liu, Y., Liu, J., Betarbet, R., Ping, L., Yin, L., et al. (2022) · Nature Neuroscience · doi:10.1038/s41593-021-00999-y · Cited by 561
Metadata
- Authors
- Lykke-Andersen, J., Shu, M.-D., & Steitz, J. A.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2000-12
- Volume
- 103
- Issue
- 7
- Pages
- 1121-1131
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2102018304
- Cited by
- 578
- References
- 2
- FWCI
- 10.03
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Lykke-Andersen, J., Shu, M.-D., & Steitz, J. A. (2000). Human Upf Proteins Target an mRNA for Nonsense-Mediated Decay When Bound Downstream of a Termination Codon. Cell. https://doi.org/10.1016/s0092-8674(00)00214-2
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