The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
A Novel Spliceosome Containing U11, U12, and U5 snRNPs Excises a Minor Class (AT–AC) Intron In Vitro
Open accessAbstract
A minor class of introns with noncanonical splice (AT-AC) and branch site sequences exists in metazoan protein coding genes. We have established a HeLa cell in vitro system that accurately splices a pre-mRNA substrate containing such an intron from the human P120 gene. Splicing occurs via a lariat intermediate whose branch site A residue is predicted to bulge from a duplex formed with the low abundance U12 small nuclear ribonucleoprotein (snRNP), which we confirm by psoralen cross-linking. Native gel electrophoresis reveals that U11, U12, and U5 snRNPs assemble onto the P120 pre-mRNA to form splicing complexes. Inhibition of P120 splicing by 2'-O-methyl oligonucleotides complementary to U12 or U5 demonstrates that U12 and U5 snRNPs perform essential roles in the AT-AC spliceosome.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Exon Recognition in Vertebrate Splicing
Berget, S. M. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.6.2411 · Cited by 1,086
Cimino, G. D., Gamper, H. B., Isaacs, S. T., & Hearst, J. E. (1985) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.54.070185.005443 · Cited by 744
Protein–protein interactions and 5'-splice-site recognition in mammalian mRNA precursors
Kohtz, J. D., Jamison, S. F., Will, C. L., Zuo, P., Lührmann, R., Garcia-Blanco, M. A., & Manley, J. L. (1994) · Nature · doi:10.1038/368119a0 · Cited by 623
Madhani, H. D., & Guthrie, C. (1992) · Cell · doi:10.1016/0092-8674(92)90556-r · Cited by 433
Messenger RNA Splicing in Yeast: Clues to Why the Spliceosome Is a Ribonucleoprotein
Guthrie, C. (1991) · Science · doi:10.1126/science.1853200 · Cited by 418
U5 snRNA interacts with exon sequences at 5′ and 3′ splice sites
Newman, A. J., & Norman, C. (1992) · Cell · doi:10.1016/0092-8674(92)90149-7 · Cited by 360
The U5 and U6 Small Nuclear RNAs as Active Site Components of the Spliceosome
Sontheimer, E. J., & Steitz, J. A. (1993) · Science · doi:10.1126/science.8266094 · Cited by 347
Self-citationA reappraisal of non-consensus mRNA splice sites
Jackson, L. J. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.14.3795 · Cited by 318
Mutations in U6 snRNA that Alter Splice Site Specificity: Implications for the Active Site
Lesser, C. F., & Guthrie, C. (1993) · Science · doi:10.1126/science.8266093 · Cited by 280
Wyatt, J. R., Sontheimer, E. J., & Steitz, J. A. (1992) · Genes & Development · doi:10.1101/gad.6.12b.2542 · Cited by 269
Self-citation
Cited by
Alternative splicing: a pivotal step between eukaryotic transcription and translation
Kornblihtt, A. R., Schor, I. E., Alló, M., Dujardin, G., Petrillo, E., & Muñoz, M. J. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3525 · Cited by 853
U1 snRNP protects pre-mRNAs from premature cleavage and polyadenylation
Kaida, D., Berg, M. G., Younis, I., Kasim, M., Singh, L. N., Wan, L., & Dreyfuss, G. (2010) · Nature · doi:10.1038/nature09479 · Cited by 638
The significant other: splicing by the minor spliceosome
Turunen, J. J., Niemelä, E. H., Verma, B., & Frilander, M. J. (2013) · WIREs RNA · doi:10.1002/wrna.1141 · Cited by 349
Protein functions in pre-mRNA splicing
Will, C. L., & Lührmann, R. (1997) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(97)80003-8 · Cited by 271
Mutation Detection in the and Alleles of the Sodium Channel
Kohrman, D. C., Harris, J. B., & Meisler, M. H. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.29.17576 · Cited by 140
Metadata
- Authors
- Tarn, W.-Y., & Steitz, J. A.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1996-03
- Volume
- 84
- Issue
- 5
- Pages
- 801-811
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2036969473
- Cited by
- 340
- References
- 29
- FWCI
- 7.67
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A Novel Spliceosome Containing U11, U12, and U5 snRNPs Excises a Minor Class (AT–AC) Intron In Vitro. https://app.cochat.ai/papers/10.1016/s0092-8674(00)81057-0
- The paper
- Tarn, W.-Y., & Steitz, J. A. (1996). A Novel Spliceosome Containing U11, U12, and U5 snRNPs Excises a Minor Class (AT–AC) Intron In Vitro. Cell. https://doi.org/10.1016/s0092-8674(00)81057-0
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
