The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Mutations in U6 snRNA that Alter Splice Site Specificity: Implications for the Active Site
Abstract
What determines the precise sites of cleavage in the two transesterification reactions of messenger RNA (mRNA) splicing is a major unsolved question. Mutation of the invariant G (guanosine) at position 5 of 5′ splice sites in Saccharomyces cerevisiae introns activates cleavage at nearby aberrant sites. A genetic approach was used to test the hypothesis that a base-pairing interaction between the 5′ splice site and the invariant ACAGAG sequence of U6 is a determinant of 5′ splice site choice. Mutations in U6 or the intron (or both) that were predicted to stabilize the interaction suppressed aberrant cleavage and increased normal cleavage. In addition, a mutation in the ACAGAG sequence suppressed mutations of the 3′ splice site dinucleotide. These data can fit a model for the spliceosomal active site comprised of a set of RNA-RNA interactions between the intron, U2 and U6.
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Cites
Sikorski, R. S., & Hieter, P. (1989) · Genetics · doi:10.1093/genetics/122.1.19 · Cited by 8,664
A general two-metal-ion mechanism for catalytic RNA
Steitz, T. A., & Steitz, J. A. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.14.6498 · Cited by 1,171
A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation
Zhuang, Y., & Weiner, A. M. (1986) · Cell · doi:10.1016/0092-8674(86)90064-4 · Cited by 722
Biochemical Mechanisms of Constitutive and Regulated Pre-mRNA Splicing
Green, M. R. (1991) · Annual Review of Cell Biology · doi:10.1146/annurev.cb.07.110191.003015 · Cited by 684
Parker, R., Siliciano, P. G., & Guthrie, C. (1987) · Cell · doi:10.1016/0092-8674(87)90564-2 · Cited by 488
Self-citationGuthrie, C., & Patterson, B. (1988) · Annual Review of Genetics · doi:10.1146/annurev.ge.22.120188.002131 · Cited by 467
Self-citationMadhani, H. D., & Guthrie, C. (1992) · Cell · doi:10.1016/0092-8674(92)90556-r · Cited by 433
Self-citationMessenger RNA Splicing in Yeast: Clues to Why the Spliceosome Is a Ribonucleoprotein
Guthrie, C. (1991) · Science · doi:10.1126/science.1853200 · Cited by 418
Self-citationSeraphin, B., & Rosbash, M. (1989) · Cell · doi:10.1016/0092-8674(89)90296-1 · Cited by 380
Interactions of Small Nuclear RNA's with Precursor Messenger RNA During in Vitro Splicing
Wassarman, D. A., & Steitz, J. A. (1992) · Science · doi:10.1126/science.1411506 · Cited by 362
Cited by
RNA Splicing by the Spliceosome
Wilkinson, M. E., Charenton, C., & Nagai, K. (2020) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-091719-064225 · Cited by 718
Dynamic RNA-RNA interactions in the spliceosome
Madhani, H. D., & Guthrie, C. (1994) · Annual Review of Genetics · doi:10.1146/annurev.ge.28.120194.000245 · Cited by 357
Self-citationThe U5 and U6 Small Nuclear RNAs as Active Site Components of the Spliceosome
Sontheimer, E. J., & Steitz, J. A. (1993) · Science · doi:10.1126/science.8266094 · Cited by 347
A Novel Spliceosome Containing U11, U12, and U5 snRNPs Excises a Minor Class (AT–AC) Intron In Vitro
Tarn, W.-Y., & Steitz, J. A. (1996) · Cell · doi:10.1016/s0092-8674(00)81057-0 · Cited by 340
An RNA Switch at the 5′ Splice Site Requires ATP and the DEAD Box Protein Prp28p
Staley, J. P., & Guthrie, C. (1999) · Molecular Cell · doi:10.1016/s1097-2765(00)80174-4 · Cited by 339
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Metadata
- Authors
- Lesser, C. F., & Guthrie, C.
- Venue
- Science
- Published
- 1993-12-24
- Volume
- 262
- Issue
- 5142
- Pages
- 1982-1988
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1968843636
- Cited by
- 280
- References
- 38
- FWCI
- 8.87
- Type
- journal article
- Language
- en
- Open access
- closed
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
