The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Yeast heat shock factor is an essential DNA-binding protein that exhibits temperature-dependent phosphorylation
Abstract
Heat shock promoters contain one or more binding sites for a specific heat shock factor (HSF). We report the cloning and sequence of the gene encoding yeast HSF, and demonstrate that HSF is required for growth at normal temperatures (15 degrees C-30 degrees C). The activity of a promoter containing a synthetic HSF binding site varies over a 200-fold range between 15 degrees C and 39 degrees C (heat shock). This change in activity is accompanied by multiple changes in the phosphorylation state of HSF, but all forms of HSF are able to bind DNA. We propose that the expression of heat shock genes in yeast is modulated by phosphorylation of DNA-bound HSF, and that this leads to a more efficient interaction of the factor with other components of the transcriptional machinery.
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“Hsf1 is activated by hyperphosphorylation in response to specific environmental cues, which in some fungi include glucose starvation, superoxide, oxygen tension and changes in membrane lipid composition as well as heat shock10,39,45,46.”
From Fungal Hsp90: a biological transistor that tunes cellular outputs to thermal inputs (2012) · cites this paper as [39]
In the citing paper
“Hsf1 is essential for viability in yeasts10,38,39, and is required for the basal expression of HSP genes10,39,40.”
From Fungal Hsp90: a biological transistor that tunes cellular outputs to thermal inputs (2012) · cites this paper as [39]
Citations
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Metadata
- Authors
- Sorger, P. K., & Pelham, H. R. B.
- Affiliations
- MRC Laboratory of Molecular Biology (GB)
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1988-09
- Volume
- 54
- Issue
- 6
- Pages
- 855-864
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2005131012
- Cited by
- 832
- References
- 27
- Citations by year
- 2026: 8 · 2025: 6 · 2024: 12 · 2023: 6 · 2022: 21 · 2021: 10 · 2020: 16 · 2019: 11 · 2018: 15 · 2017: 10 · 2016: 22 · 2015: 9 · 2014: 15 · 2013: 25 · 2012: 17
- FWCI
- 24.28
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- heat shock transcription factor, Saccharomyces cerevisiae, heat shock promoter, protein phosphorylation, DNA binding, heat shock gene expression, transcriptional regulation, gene cloning
- MeSH terms
- Amino Acid Sequence, Base Sequence, Cell Survival, Cloning, Molecular, DNA-Binding Proteins, Gene Expression Regulation, Hot Temperature, Heat-Shock Proteins, Molecular Sequence Data, Molecular Weight, Phosphoproteins, Phosphorylation, Saccharomyces cerevisiae, Transcription Factors, Transcription, Genetic
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