The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Activity of Porphyridium sp. polysaccharide against herpes simplex viruses in vitro and in vivo
Abstract
The cell wall sulfated polysaccharide of the red microalga Porphyridium sp. exhibited impressive antiviral activity against herpes simplex virus types 1 and 2 (HSV-1 and -2) both in vitro (cell culture) and in vivo (rats and rabbits). Depending on the concentration, this polysaccharide completely inhibited or slowed down the development of the cytopathic effect in HSV-infected cells, but did not show any cytotoxic effects on vero cells even when a concentration as high as 250 microg/ml was used. There was indirect evidence for a strong interaction between the polysaccharide and HSV and a weak interaction with the cell surface. When tested in vivo, Porphyridium sp. polysaccharide conferred significant and efficient protection against HSV-1 infection: at a concentration as low as 100 microg/ml, it prevented the appearance and development of symptoms of HSV-1 infection in rats and rabbits. The polysaccharide did not exhibit any cytotoxic effects at a concentration of 2 mg/ml in vivo.
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“Algae are considered as a good source of dietary fibers because of the presence of non-digestible polysaccharides in the algal cell wall (Figure 3) [11]. It has been shown that they possess activities, like antioxidant [12], hypoglycemic [13], antiviral [14,15], antitumor [16], and anti-inflammatory due to the presence of fucoidan and laminarin [17].”
From Overview on the Application of Modern Methods for the Extraction of Bioactive Compounds from Marine Macroalgae (2018) · cites this paper as [15]
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Cites
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Polysaccharides as antiviral agents: antiviral activity of carrageenan
González, M. E., Alarcón, B., & Carrasco, L. (1987) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.31.9.1388 · Cited by 187
INHIBITORY EFFECT OF HEPARIN ON HERPES SIMPLEX VIRUS
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Protective Effect of Seaweed Extracts for Chicken Embryos Infected with Influenza B or Mumps Virus
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Alam, A., Bibi, F., & Porwal, K. (2021) · Annals of Phytomedicine An International Journal · doi:10.54085/ap.covid19.2021.10.2.4
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Bioraffinerie de la microalgue Haematococcus pluvialis
Ba, F. (2016) · theses.fr (ABES) · doi:10.70675/71e8168fz0507z4d65z8f75zf26b8f522563
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Falaise, C. (2019) · theses.fr (ABES) · doi:10.70675/274504eez45aaz44b8za87azf43b48df5f86
Evaluation du potentiel anti-âge d'exopolysaccharides issus de microalgues
Toucheteau, C. (2020) · theses.fr (ABES) · doi:10.70675/2c74bb84z75aez46a2z92e9z9378faf67306
Marcellin-Gros, R. (2020) · theses.fr (ABES) · doi:10.70675/9eaf9c88z3bd4z4084zb8ffz1d5163213feb
Bayu, A., Rahmawati, S. I., Purbani, D. C., Noerdjito, D. R., Bustanussalam, Hapsari, Y., & Putra, M. Y. (2026) · Algal Research · doi:10.1016/j.algal.2026.104718
Duarte, M. M., Suprinovych, A., Veiga, A., Lopes, A. I., Tavaria, F. K., Morais, R. C., & Oliveira, A. L. (2026) · Gels · doi:10.3390/gels12050352
Levy‐Ontman, O., Lavi-Ohayon, T., & Wolfson, A. (2026) · Journal of Polymers and the Environment · doi:10.1007/s10924-026-03834-2
Karshan, N., Huliehel, M., & Levy‐Ontman, O. (2026) · Polymer · doi:10.1016/j.polymer.2026.130755
dos Santos, N. P., Baptista, R. M., Kubelka, B. G., D’Oca, M. G. M., de Lima, J. T., Bueno, D. T., Leitzke, A. F., & Ramos, D. F. (2026) · Frontiers in Natural Products · doi:10.3389/fntpr.2026.1884549
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Metadata
- Authors
- Huheihel, M., Ishanu, V., Tal, J., & Arad, S. (.
- Affiliations
- Ben-Gurion University of the Negev (IL)
- Corresponding author
- Huheihel, Mahmoud
- Venue
- Journal of Biochemical and Biophysical Methods
- Publisher
- Elsevier BV
- Published
- 2002-01
- Volume
- 50
- Issue
- 2-3
- Pages
- 189-200
- ISSN
- 0165-022X (ISSN-L)
- OpenAlex
- W1986624355
- Cited by
- 217
- References
- 24
- Citations by year
- 2026: 7 · 2025: 9 · 2024: 8 · 2023: 9 · 2022: 15 · 2021: 17 · 2020: 24 · 2019: 13 · 2018: 6 · 2017: 7 · 2016: 8 · 2015: 8 · 2014: 9 · 2013: 10 · 2012: 12
- FWCI
- 5.14
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biochemical and Biophysical Methods (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Health Sciences › Medicine › Infectious Diseases › Herpesvirus Infections and Treatments
- Keywords
- Porphyridium, sulfated polysaccharides, cell wall polysaccharides, antiviral activity, herpes simplex virus type 1, herpes simplex virus type 2, red microalgae, cytopathic effect inhibition, cytotoxicity, cell surface binding
- MeSH terms
- Adsorption, Rhodophyta, Animals, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Herpes Simplex, Male, Polysaccharides, Rabbits, Vero Cells, Keratitis, Herpetic, Herpesvirus 2, Human, Herpesvirus 1, Human, Rats, In Vitro Techniques
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