A rapid, nongenomic action of glucocorticoids in rat B103 neuroblastoma cells
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We report here a new example in which glucocorticoids (GCs) acted in a rapid, nongenomic way. In rat B103 neuroblastoma cells, 5-hydroxytryptamine (5-HT) was found to evoke an immediate rise in intracellular free calcium concentration ([Ca(2+)](i)). Pre-incubation of B103 cells for 5 min with corticosterone (B) or bovine serum albumin-conjugated corticosterone (B-BSA) concentration-dependently (10(-4)-10(-8) M) inhibited the peak increments in [Ca(2+)](i). Cortisol and dexamethasone had a similar effect, while deoxycorticosterone and cholesterol were ineffective. This rapid inhibitory effect of corticosterone could be mimicked by protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) and abolished completely by PKC inhibitors Ro31-8220 or GF-109203X. Neither pertussis toxin (PTX) nor nuclear GC receptor (GR) antagonist RU38486 influenced the rapid action of B. Our results suggest that GCs can modulate the 5-HT-induced Ca(2+) response in B103 cells in a membrane-initiated, nongenomic, and PKC-dependent manner.
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Glucocorticoid release during stress could further potentiate these pathways through non-genomic activation of PKC signalling, although it is not specifically known whether glucocorticoids have this effect in the PFC.
“Glucocorticoid release during stress could further potentiate these pathways through non-genomic activation of PKC signalling103 (although it is not specifically known whether glucocorticoids have this effect in the PFC).”
From Stress signalling pathways that impair prefrontal cortex structure and function (2009) · cites this paper as [103]
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A corticosteroid receptor in neuronal membranes
Orchinik, M., Murray, T. F., & Moore, F. L. (1991) · Science · doi:10.1126/science.2063198 · Cited by 523 · see citing works
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Stress signalling pathways that impair prefrontal cortex structure and function
Arnsten, A. F. T. (2009) · Nature Reviews Neuroscience · doi:10.1038/nrn2648 · Cited by 3,299 · see citing works
Non-genomic effects of glucocorticoids: an updated view
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Nongenomic actions of adrenal steroids in the central nervous system
Evanson, N. K., Herman, J. P., Sakai, R. R., & Krause, E. G. (2010) · Journal of Neuroendocrinology · doi:10.1111/j.1365-2826.2010.02000.x · Cited by 76 · see citing works
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- Authors
- Han, J.-Z., Lin, W., Lou, S.-J., Qiu, J., & Chen, Y.-Z.
- Affiliations
- Second Military Medical University (CN)
- Venue
- Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
- Publisher
- Elsevier BV
- Published
- 2002-08
- Volume
- 1591
- Issue
- 1-3
- Pages
- 21-27
- ISSN
- 0167-4889 (ISSN-L)
- OpenAlex
- W2004899596
- Cited by
- 22
- References
- 1
- Citations by year
- 2023: 2 · 2022: 1 · 2019: 1 · 2018: 1 · 2012: 3
- FWCI
- 0.53
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biochimica et Biophysica Acta (BBA) - Molecular Cell Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Key Research and Development Program of China
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- corticosterone, intracellular calcium, serotonin, protein kinase C, membrane-initiated signaling, glucocorticoid receptor, phorbol myristate acetate
- MeSH terms
- Animals, Calcium, Drug Interactions, Glucocorticoids, Neuroblastoma, Serotonin, Tumor Cells, Cultured, Signal Transduction, Rats
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