Microscopic localization of PEG-liposomes in a rat model of focal infection
Abstract
In the present study the microscopic localization of polyethylene glycol (PEG) liposomes in infected tissues was studied with both light microscopy (LM) and transmission electron microscopy (TEM) in rats with focal intramuscular Staphylococcus aureus infection. PEG-liposomes containing colloidal gold were prepared and injected intravenously in rats with focal S. aureus infection and tissues were dissected at 24 h post injection. Sections were cut and liposomes were visualized for microscopic evaluation using silver enhancement. Uptake of PEG-liposomes was visualized by both scintigraphy and LM in the abscess, liver and spleen. In the infected area, the liposomes were mainly found in the vicinity of blood vessels. TEM showed that the liposomes were localized in the macrophages and to a lesser extent in endothelial cells in the infectious tissue. In the liver, the liposomes appeared mainly localized in Kupffer cells. In the spleen, uptake was only seen in cells of the red pulp and in cells around the central arteries. Our microscopic observations indicate that uptake and retention of PEG-liposomes in the infectious focus is a result of enhanced extravasation due to increased vascular permeability and subsequent phagocytosis of PEG-liposomes by macrophages in the infected tissue.
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In one study, there was an ~40-fold greater liposome concentration in the abscess compared with the muscle.
“In one study 48 , there was an ~40-fold greater liposome concentration (per cent injected dose per gram of tissue) in the abscess compared with the muscle.”
From Nanotechnology approaches for global infectious diseases (2021) · cites this paper as [48]
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Nanotechnology approaches for global infectious diseases
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- Authors
- Laverman, P., Dams, E. T. M., Storm, G., Hafmans, T. G., Croes, H. J., Oyen, W. J. G., Corstens, F. H. M., & Boerman, O. C.
- Affiliations
- Radboud University Nijmegen (NL)
- Utrecht University (NL)
- Corresponding author
- Laverman, Peter
- Venue
- Journal of Controlled Release
- Publisher
- Elsevier BV
- Published
- 2001-08
- Volume
- 75
- Issue
- 3
- Pages
- 347-355
- ISSN
- 0168-3659 (ISSN-L)
- OpenAlex
- W2011243571
- Cited by
- 53
- References
- 20
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 1 · 2022: 2 · 2021: 4 · 2020: 4 · 2019: 3 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 3 · 2014: 2 · 2013: 2 · 2012: 2
- FWCI
- 1.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Controlled Release (publishedVersion)
- PubMed (publishedVersion)
- Radboud Repository (Radboud University) (submittedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- PEG-liposomes, Staphylococcus aureus infection, abscess, gold nanoparticles, macrophages, Kupffer cells, vascular permeability, extravasation, phagocytosis, liposome uptake, liver, spleen, silver enhancement, scintigraphy, focal infection
- MeSH terms
- Abscess, Animals, Drug Carriers, Liposomes, Liver, Male, Microscopy, Electron, Polyethylene Glycols, Spleen, Staphylococcal Infections, Tissue Distribution, Rats, Wistar, Rats
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