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Heterologous protein expression in the methylotrophic yeast Pichia pastoris
Open accessAbstract
During the past 15 years, the methylotrophic yeast Pichia pastoris has developed into a highly successful system for the production of a variety of heterologous proteins. The increasing popularity of this particular expression system can be attributed to several factors, most importantly: (1) the simplicity of techniques needed for the molecular genetic manipulation of P. pastoris and their similarity to those of Saccharomyces cerevisiae, one of the most well-characterized experimental systems in modern biology; (2) the ability of P. pastoris to produce foreign proteins at high levels, either intracellularly or extracellularly; (3) the capability of performing many eukaryotic post-translational modifications, such as glycosylation, disulfide bond formation and proteolytic processing; and (4) the availability of the expression system as a commercially available kit. In this paper, we review the P. pastoris expression system: how it was developed, how it works, and what proteins have been produced. We also describe new promoters and auxotrophic marker/host strain combinations which extend the usefulness of the system.
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Metadata
- Authors
- Cereghino, J.
- Venue
- FEMS Microbiology Reviews
- Publisher
- Wiley
- Published
- 2000-01
- Volume
- 24
- Issue
- 1
- Pages
- 45-66
- ISSN
- 0168-6445 (ISSN-L)
- OpenAlex
- W2120357935
- Cited by
- 2,039
- References
- 1
- FWCI
- 16.90
- Type
- journal article
- Language
- en
- Open access
- bronze
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