The DOI matches its Crossref record; 24 of the 25 references it cites were found in a registry or an index.
Expression of a chimeric farnesyl diphosphate synthase gene in Artemisia annua L. transgenic plants via Agrobacterium tumefaciens-mediated transformation
Abstract
An Agrobacterium tumefaciens-mediated transformation system was developed for Artemisia annua L. Using this system a cDNA encoding farnesyl diphosphate synthase (FDS placed under a CaMV 35S promoter) was transferred into A. annua via A. tumefaciens strain LB4404. Leaf or leaf discs were used as explants to be infected with A. tumefaciens and an optimal concentration of 20 mg/l kanamycin was applied to select kanamycin resistant shoots. Forty-five lines of resistance kanamycin shoots transformed with FDS were established. Analysis of PCR showed that at least 20 shoots transformed with the FDS gene were PCR positive. Southern blot analysis suggested the foreign FDS gene had been integrated into the A. annua genome, and Northern blot analysis revealed that the foreign FDS gene expressed at the transcriptional level in five shoot lines (F-1, F-4, F-61, F-62 and F-73 shoot lines). Analysis of artemisinin demonstrated that about 8 approximately 10 mg/g DW of artemisinin were then detected in transgenic plants regenerated from five shoot lines, this is about 2-3 times higher than that in the control.
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“Transgenic plants produced by introduction of a heterologous FPS gene from Gossypium arboreum resulted in 2-3 times more artemisinin compared to non-transgenic plants (Chen et al. 2000).”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Chen et al. 2000
In the citing paper
“A. annua transgenic lines with FPS over-expression showed a three-fold increase in artemisinin (Banyai et al. 2010a; Chen et al. 2000).”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Chen et al. 2000
Citations
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Metadata
- Authors
- Chen, D.-H., Ye, H.-C., & Li, G.-F.
- Venue
- Plant Science
- Publisher
- Elsevier BV
- Published
- 2000-06
- Volume
- 155
- Issue
- 2
- Pages
- 179-185
- ISSN
- 0168-9452 (ISSN-L)
- OpenAlex
- W1988086615
- Cited by
- 225
- References
- 25
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 8 · 2023: 4 · 2022: 10 · 2021: 2 · 2020: 8 · 2019: 8 · 2018: 9 · 2017: 8 · 2016: 15 · 2015: 10 · 2014: 12 · 2013: 17 · 2012: 10
- FWCI
- 1.93
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Plant Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Keywords
- farnesyl diphosphate synthase, Artemisia annua, transgenic plants, Agrobacterium-mediated transformation, artemisinin biosynthesis, CaMV 35S promoter, kanamycin selection, leaf disc explants, transgene integration, artemisinin yield
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