Cited by 95Plant biochemistry and biosynthesisPlant tissue culture and regenerationPlant Gene Expression Analysis

Cloning and analysis of a cDNA encoding farnesyl diphosphate synthase from Artemisia annua

Matsushita, Yasuhiko, Kang, Wonkyung, Charlwood, Barry V.·1996·Gene

Abstract

A cDNA encoding farnesyl diphosphate (FPP) synthase (FPPS) has been cloned from a cDNA library of Artemisia annua. The sequence analysis showed that the cDNA encoded a protein of 343 amino acid (aa) residues with a calculated molecular weight of 39420 kDa. The deduced aa sequence of the cDNA was highly similar to FPPS from other plants, yeast and mammals, and contained the two conserved domains found in polyprenyl synthases including FPPS, geranylgeranyl diphosphate synthases and hexaprenyl diphosphate synthases. The expression of the cDNA in Escherichia coli showed enzyme activity for FPPS in vitro.

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Crossref · 10.1016/0378-1119(96)00054-6

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9 of 9 references verified0 citation errors · 0 not checkable

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Authors
Matsushita, Y., Kang, W., & Charlwood, B. V.
Affiliations
King's College London (GB)
Corresponding author
Matsushita, Yasuhiko
Venue
Gene
Publisher
Elsevier BV
Published
1996-06
Volume
172
Issue
2
Pages
207-209
ISSN
0378-1119 (ISSN-L)
OpenAlex
W2084397100
Cited by
95
References
9
Citations by year
2026: 1 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 6 · 2016: 6 · 2015: 9 · 2014: 2 · 2013: 5 · 2012: 8
FWCI
0.76
Type
journal article
Language
en
Open access
closed
Locations
  • Gene (publishedVersion)
  • PubMed (publishedVersion)
Funders
Biotechnology and Biological Sciences Research Council
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant Gene Expression Analysis
Keywords
farnesyl diphosphate synthase, Artemisia annua, cDNA cloning, conserved domains, geranylgeranyl diphosphate synthase, heterologous expression in Escherichia coli, enzyme activity, terpene biosynthesis
MeSH terms
Amino Acid Sequence, Base Sequence, Cloning, Molecular, Molecular Sequence Data, Plants, Medicinal, Transferases, DNA, Complementary, Alkyl and Aryl Transferases, Geranyltranstransferase

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