Cloning and analysis of a cDNA encoding farnesyl diphosphate synthase from Artemisia annua
Abstract
A cDNA encoding farnesyl diphosphate (FPP) synthase (FPPS) has been cloned from a cDNA library of Artemisia annua. The sequence analysis showed that the cDNA encoded a protein of 343 amino acid (aa) residues with a calculated molecular weight of 39420 kDa. The deduced aa sequence of the cDNA was highly similar to FPPS from other plants, yeast and mammals, and contained the two conserved domains found in polyprenyl synthases including FPPS, geranylgeranyl diphosphate synthases and hexaprenyl diphosphate synthases. The expression of the cDNA in Escherichia coli showed enzyme activity for FPPS in vitro.
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13C labeling studies have characterized the photosynthetic mechanism of A. annua as C3.
“and 13C labeling studies have also been used to characterize the photosynthetic mechanism of A. annua as C3 [289].”
From The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao) (2010) · cites this paper as [289]
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- Authors
- Matsushita, Y., Kang, W., & Charlwood, B. V.
- Affiliations
- King's College London (GB)
- Corresponding author
- Matsushita, Yasuhiko
- Venue
- Gene
- Publisher
- Elsevier BV
- Published
- 1996-06
- Volume
- 172
- Issue
- 2
- Pages
- 207-209
- ISSN
- 0378-1119 (ISSN-L)
- OpenAlex
- W2084397100
- Cited by
- 95
- References
- 9
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 6 · 2016: 6 · 2015: 9 · 2014: 2 · 2013: 5 · 2012: 8
- FWCI
- 0.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Gene (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Biotechnology and Biological Sciences Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant Gene Expression Analysis
- Keywords
- farnesyl diphosphate synthase, Artemisia annua, cDNA cloning, conserved domains, geranylgeranyl diphosphate synthase, heterologous expression in Escherichia coli, enzyme activity, terpene biosynthesis
- MeSH terms
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Molecular Sequence Data, Plants, Medicinal, Transferases, DNA, Complementary, Alkyl and Aryl Transferases, Geranyltranstransferase
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Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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