Cloning and expression of a novel, truncated, progesterone receptor

Saner, Karla J.; Welter, Brenda H.; Zhang, Fan; Hansen, Elizabeth; Dupont, Barbara; Wei, Yangzhan; Price, Thomas M.

doi:10.1016/s0303-7207(02)00380-5

Cited by 64Estrogen and related hormone effectsProstate Cancer Treatment and ResearchPeroxisome Proliferator-Activated Receptors

Cloning and expression of a novel, truncated, progesterone receptor

Saner, Karla J., Welter, Brenda H., Zhang, Fan, Hansen, Elizabeth, Dupont, Barbara, Wei, Yangzhan, Price, Thomas M.·2003·Molecular and Cellular Endocrinology

DOI 10.1016/s0303-7207(02)00380-5

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Abstract

Progesterone acts via two specific receptors to affect gene transcription in target tissues. Progesterone receptor (PR) B contains 933 amino acids while PR A is a truncated version lacking the initial 164 amino acids. We have cloned a novel, truncated PR from both human adipose and aortic cDNA libraries. This cDNA encodes a predicted protein of 314 amino acids, termed PR-M. Initiation of transcription of PR-M occurs in intron 3, with the initial exon identical to exon 4 of the genomic PRs, except for a novel 16 amino acid amino-terminal sequence, consistent with a signal peptide. The remainder of PR-M is identical to the genomic PR. Transcript for this protein was identified by RT-PCR in human aortic endothelial cells and T47D breast cancer cells. Expression of PR-M in Sf 9 insect cells results in a 38-kDa protein, demonstrated in human aortic endothelial cells and T47D breast cancer cells. The function of PR-M remains to be determined. The presence of a signal peptide and the lack of a DNA binding region suggests a non-genomic action.

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Authors: Saner, K. J., Welter, B. H., Zhang, F., Hansen, E., Dupont, B., Wei, Y., & Price, T. M.
Affiliations: Clemson University (US)
Greenwood Genetic Center (US)
Prisma Health (US)
Corresponding author: Price, Thomas M.
ORCID: Zhang, Fan
Price, Thomas M.
Venue: Molecular and Cellular Endocrinology
Publisher: Elsevier BV
Published: 2003-02
Volume: 200
Issue: 1-2
Pages: 155-163
ISSN: 0303-7207 (ISSN-L)
DOI: 10.1016/s0303-7207(02)00380-5
OpenAlex: W2007416471
Cited by: 64
References: 46
Citations by year: 2026: 2 · 2025: 1 · 2023: 4 · 2022: 1 · 2021: 4 · 2020: 3 · 2019: 3 · 2017: 4 · 2016: 2 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 2
FWCI: 2.82
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Molecular and Cellular Endocrinology (publishedVersion)
PubMed (publishedVersion)
Funders: American Heart Association
Elsa U. Pardee Foundation
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Prostate Cancer Treatment and Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
Keywords: progesterone receptor, progesterone receptor A, progesterone receptor B, signal peptide, alternative transcription initiation, intron 3, aortic endothelial cells, breast cancer cells, cDNA cloning
MeSH terms: Adipose Tissue, Animals, Base Sequence, Cloning, Molecular, Female, Humans, Molecular Sequence Data, Receptors, Progesterone, Gene Library, Protein Structure, Tertiary, Protein Sorting Signals, Endothelial Cells, Cell Line, Tumor

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Cloning and expression of a novel, truncated, progesterone receptor

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