Interruption of supramammillohippocampal afferents prevents the genesis and spread of limbic seizures in the hippocampus via a disinhibition mechanism
Abstract
In this study we describe the preventive effect of interruption of the supramammillohippocampal afferents on the Fos expression in the forebrain and epileptic discharges in the hippocampal electroencephalogram in rat model of kainic acid-induced limbic seizure. Little was known about the contribution of different degrees of neural activity of hippocampal principal cells to the genesis and spread of limbic seizures in the forebrain structures. Following kainic acid injection to the amygdala with or without concurrent injection of muscimol to the supramammillary nucleus, behavioral changes and electroencephalograms were observed in freely moving rats. The animals were processed for Fos immunocytochemical analysis at several time points. The latest expression of Fos at 2h was seen in hippocampal CA1-CA3, ventrolateral thalamic nuclei and mediodorsal caudate putamen, while the early Fos expression at 0.5h was seen in the piriform, entorhinal and other cortices, the thalamic midline nuclei and hypothalamic nuclei. Muscimol injection to the supramammillary nucleus prevented Fos expression in the CA1-CA3 region and reduced that in the forebrain regions with the latest Fos expression, but did not affect Fos expression in other forebrain regions with early Fos expression. This treatment also eliminated epileptic discharges and attenuated all waves in hippocampus. These findings indicate that an acute interruption of the facilitatory hypothalamic afferents by intrasupramammillary injec…
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CA2 neurons are the only hippocampal target of the supramammillary nucleus, a structure involved in controlling theta rhythm and spread of epileptic activity in the hippocampus.
“Finally, CA2 neurons are the only hippocampal target of the supramammillary nucleus (which also targets the dentate gyrus), a structure involved in controlling the frequency of the theta rhythm (Pan and McNaughton, 2002) and the spread of epileptic activity (Saji et al., 2000) in the hippocampus.”
From Strong CA2 Pyramidal Neuron Synapses Define a Powerful Disynaptic Cortico-Hippocampal Loop (2010) · cites this paper as Saji et al., 2000
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Strong CA2 Pyramidal Neuron Synapses Define a Powerful Disynaptic Cortico-Hippocampal Loop
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- Authors
- Saji, M., Kobayashi, S., Ohno, K., & Sekino, Y.
- Affiliations
- Kitasato University (JP)
- Tottori University (JP)
- Gunma University (JP)
- Venue
- Neuroscience
- Publisher
- Elsevier BV
- Published
- 2000-05
- Volume
- 97
- Issue
- 3
- Pages
- 437-445
- ISSN
- 0306-4522 (ISSN-L)
- OpenAlex
- W2088804977
- Cited by
- 20
- References
- 42
- Citations by year
- 2022: 1 · 2017: 1 · 2014: 1 · 2013: 1 · 2012: 1
- FWCI
- 0.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Health and Welfare (C-09671424)
- Ministry of Education, Culture, Sports, Science and Technology
- Topics
- Health Sciences › Medicine › Psychiatry and Mental health › Epilepsy research and treatment
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- supramammillary nucleus, limbic seizures, kainic acid-induced epilepsy, muscimol, Fos expression, disinhibition, dentate gyrus, amygdala, thalamus, hypothalamus, epileptic discharges, seizure prevention
- MeSH terms
- Afferent Pathways, Amygdala, Animals, Behavior, Animal, Denervation, Electroencephalography, Epilepsy, Hippocampus, Hypothalamus, Kainic Acid, Male, Muscimol, Neural Inhibition, Neurons, Receptors, GABA-A, Proto-Oncogene Proteins c-fos, Rats, Wistar, Entorhinal Cortex, Rats, GABA-A Receptor Antagonists
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