The DOI matches its Crossref record.
Identification of emetic toxin producing Bacillus cereus strains by a novel molecular assay
Abstract
Bacillus cereus causes two types of gastrointestinal diseases: emesis and diarrhea. The emetic type of the disease is attributed to the heat-stable depsipeptide cereulide and symptoms resemble Staphylococcus aureus intoxication, but there is no rapid method available to detect B. cereus strains causing this type of disease. In this study, a polymerase chain reaction (PCR) fragment of unknown function was identified, which was shown to be specific for emetic toxin producing strains of B. cereus. The sequence of this amplicon was determined and a PCR assay was developed on this basis. One hundred B. cereus isolates obtained from different food poisoning outbreaks and diverse food sources from various geographical locations and 29 strains from other species belonging to the B. cereus group were tested by this assay. In addition, 49 non-B. cereus group strains, with special emphasis on food pathogens, were used to show that the assay is specific for emetic toxin producing B. cereus strains. The presented PCR assay is the first molecular tool for the rapid detection of emetic toxin producing B. cereus strains.
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Cited by
(EFSA), E. F. S. A. (2007) · EFSA Journal · doi:10.2903/j.efsa.2007.587 · Cited by 479
Fatal Family Outbreak of Bacillus cereus -Associated Food Poisoning
Dierick, K., Van Coillie, E., Swiecicka, I., Meyfroidt, G., Devlieger, H., Meulemans, A., Hoedemaekers, G., Fourie, L., Heyndrickx, M., & Mahillon, J. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.8.4277-4279.2005 · Cited by 455
Ehling-Schulz, M., Guinebretiere, M.-H., Monthán, A., Berge, O., Fricker, M., & Svensson, B. (2006) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2006.00320.x · Cited by 274
Self-citationGovasmark, E., Stäb, J., Holen, B., Hoornstra, D., Nesbakk, T., & Salkinoja-Salonen, M. (2011) · Waste Management · doi:10.1016/j.wasman.2011.07.025 · Cited by 125
HUMBLOT, C., PEREZ-PULIDO, R., AKAKI, D., LOISEAU, G., & GUYOT, J.-P. (2012) · Journal of Food Protection · doi:10.4315/0362-028x.jfp-11-450 · Cited by 10
Metadata
- Authors
- Ehling-Schulz, M., Fricker, M., & Scherer, S.
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-03
- Volume
- 232
- Issue
- 2
- Pages
- 189-195
- ISSN
- 0378-1097 (ISSN-L)
- 1574-6968
- OpenAlex
- W2160257247
- Cited by
- 189
- References
- 0
- FWCI
- 4.05
- Type
- journal article
- Language
- en
- Open access
- closed
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- Ehling-Schulz, M., Fricker, M., & Scherer, S. (2004). Identification of emetic toxin producing Bacillus cereus strains by a novel molecular assay. FEMS Microbiology Letters. https://doi.org/10.1016/s0378-1097(04)00066-7
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