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Cited by 274

The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.

Toxin gene profiling of enterotoxic and emetic Bacillus cereus: Toxin gene profiling of enterotoxic and emetic B. cereus

Ehling-Schulz, Monika, Guinebretiere, Marie-Hélène, Monthán, Amanda, Berge, Odile, Fricker, Martina, Svensson, Birgitta·2006·FEMS Microbiology Letters

Abstract

Very different toxins are responsible for the two types of gastrointestinal diseases caused by Bacillus cereus: the diarrhoeal syndrome is linked to nonhemolytic enterotoxin NHE, hemolytic enterotoxin HBL, and cytotoxin K, whereas emesis is caused by the action of the depsipeptide toxin cereulide. The recently identified cereulide synthetase genes permitted development of a molecular assay that targets all toxins known to be involved in food poisoning in a single reaction, using only four different sets of primers. The enterotoxin genes of 49 strains, belonging to different phylogenetic branches of the B. cereus group, were partially sequenced to encompass the molecular diversity of these genes. The sequence alignments illustrated the high molecular polymorphism of B. cereus enterotoxin genes, which is necessary to consider when establishing PCR systems. Primers directed towards the enterotoxin complex genes were located in different CDSs of the corresponding operons to target two toxin genes with one single set of primers. The specificity of the assay was assessed using a panel of B. cereus strains with known toxin profiles and was successfully applied to characterize strains from food and clinical diagnostic labs as well as for the toxin gene profiling of B. cereus isolated from silo tank populations.

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Metadata

Authors
Ehling-Schulz, M., Guinebretiere, M.-H., Monthán, A., Berge, O., Fricker, M., & Svensson, B.
Venue
FEMS Microbiology Letters
Published
2006-07
Volume
260
Issue
2
Pages
232-240
ISSN
  • 0378-1097 (ISSN-L)
  • 1574-6968
OpenAlex
W1533665911
Cited by
274
References
17
FWCI
3.44
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Toxin gene profiling of enterotoxic and emetic Bacillus cereus: Toxin gene profiling of enterotoxic and emetic B. cereus. https://app.cochat.ai/papers/10.1111/j.1574-6968.2006.00320.x
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Ehling-Schulz, M., Guinebretiere, M.-H., Monthán, A., Berge, O., Fricker, M., & Svensson, B. (2006). Toxin gene profiling of enterotoxic and emetic Bacillus cereus: Toxin gene profiling of enterotoxic and emetic B. cereus. FEMS Microbiology Letters. https://doi.org/10.1111/j.1574-6968.2006.00320.x

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