Imprinted methylation and its effect on expression of the mouse Zfp127 gene
Abstract
The Zfp127 gene is located on mouse chromosome 7 in an imprinted region that is homologous to the 2-Mb Prader-Willi and Angelman Syndromes region on human chromosome 15q11-q13. Here, we show that the gene is differentially methylated, the maternal allele being methylated and the paternal allele being unmethylated. This maternal methylation is established promptly after fertilization prior to syngamy. We also provide data that demonstrate the significance of methylation in the paternal expression of the gene. The expression of the Zfp127 gene in methyltransferase-deficient mice is significantly higher, suggesting that the gene is biallelically expressed in these mice. The data presented here will help to understand the mechanism by which the monoallelic expression of the entire 2-Mb Prader-Willi and Angelman Syndrome region is regulated.
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The maternal MKRN3 allele is imprinted and only the paternal allele is expressed.
“The maternal MKRN3 allele is imprinted and only the paternal allele is expressed.68–70”
From Pubertal development and regulation (2016) · cites this paper as [70]
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Puberty 1 Pubertal development and regulation
Abreu, A. P., & Kaiser, U. B. (2016)
- Authors
- Hershko, A., Razin, A., & Shemer, R.
- Affiliations
- Hebrew University of Jerusalem (IL)
- Corresponding author
- Shemer, R
- ORCID
- Venue
- Gene
- Publisher
- Elsevier BV
- Published
- 1999-07
- Volume
- 234
- Issue
- 2
- Pages
- 323-327
- ISSN
- 0378-1119 (ISSN-L)
- OpenAlex
- W2114722116
- Cited by
- 25
- References
- 13
- Citations by year
- 2021: 1 · 2020: 2 · 2019: 1 · 2018: 1 · 2016: 4 · 2015: 1 · 2014: 2 · 2013: 3 · 2012: 1
- FWCI
- 0.18
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Gene (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Syndromes and Imprinting
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- genomic imprinting, DNA methylation, monoallelic expression, Prader-Willi syndrome, Angelman syndrome, chromosome 7, chromosome 15q11-q13, paternal allele expression, gene expression regulation
- MeSH terms
- Alleles, Animals, DNA, Female, Gene Expression Regulation, Genes, Male, Mice, Inbred BALB C, Mice, Inbred C57BL, Ribonucleoproteins, Genomic Imprinting, DNA Methylation, Ubiquitin-Protein Ligases, Mice
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