The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Determination of Optimal Conditions for Synthesis of Peroxynitrite by Mixing Acidified Hydrogen Peroxide with Nitrite
Abstract
The measured parameters for the formation of peroxynitrous acid via the reaction of acidified hydrogen peroxide with nitrous acid and its self-decomposition corroborate with an earlier suggested mechanism in which H2NO2+ nitrosates H2O2. The activation energies for the formation and decay of peroxynitrous acid have been determined to be 15 and 19 kcal/mol, respectively. We found that perchlorate, nitrate, sulfate and phosphate ions have no effect on the formation and decay rates, whereas chloride ions enhance the rate of the formation of peroxynitrous acid at low peroxide concentrations, and have no effect at high peroxide concentrations. This suggests that at relatively low concentration of H2O2, Cl- competes with H2O2 for H2NO+ to yield NOCl, which may also nitrosate H2O2. Simulation of the experimentally observed parameters for the decay and formation rates suggests that it is not possible to obtain 100% yield of peroxynitrite under any condition. High yields of peroxynitrite were obtained at room temperature using an efficient double mixer where acidified peroxide was mixed with nitrite; after an appropriate delay, the reaction was quenched with strong alkali. An excess of more than 10% of H2O2 over nitrite, or vice versa, is sufficient to get ca. 85-90% of peroxynitrite, almost free from nitrite or H2O2, respectively. The results also suggest that conventional use of ice-cold solutions of the reactants and the alkali solutions is not required if an efficient mixer and appropriate quenching times are available.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“HO mediates the degradation of heme in mammals. This enzyme catalyzes the degradation of the protoporphyrin IX (heme b) to biliverdin (805, 806).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [806]
In the citing paper
“This enzyme catalyzes the degradation of the protoporphyrin IX (heme b) to biliverdin (805, 806).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [806]
Citations
Cites
Beckman, J. S., Beckman, T. W., Chen, J., Marshall, P. A., & Freeman, B. A. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.4.1620 · Cited by 7,029
Nitric oxide, superoxide, and peroxynitrite: the good, the bad, and ugly
Beckman, J. S., & Koppenol, W. H. (1996) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1996.271.5.c1424 · Cited by 5,513
The chemistry of peroxynitrite: A product from the reaction of nitric oxide with superoxide
Pryor, W. A., & Squadrito, G. L. (1995) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.1995.268.5.l699 · Cited by 1,511
Peroxynitrite, a cloaked oxidant formed by nitric oxide and superoxide
Koppenol, W. H., Moreno, J. J., Pryor, W. A., Ischiropoulos, H., & Beckman, J. S. (1992) · Chemical Research in Toxicology · doi:10.1021/tx00030a017 · Cited by 1,338
[23] Oxidative chemistry of peroxynitrite
Beckman, J. S., Chen, J., Ischiropoulos, H., & Crow, J. P. (1994) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(94)33026-3 · Cited by 973
H2O2 Determination by the I3- Method and by KMnO4 Titration
Klassen, N. V., Marchington, D., & Mcgowan, H. (1994) · Analytical Chemistry · doi:10.1021/ac00090a020 · Cited by 967
Pathological implications of nitric oxide, superoxide and peroxynitrite formation
Beckman, J. S., & Crow, J. P. (1993) · Biochemical Society Transactions · doi:10.1042/bst0210330 · Cited by 674
The reaction of NO· with O2·− and HO2·−: A pulse radiolysis study
Goldstein, S., & Czapski, G. (1995) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(95)00034-u · Cited by 398
Self-citationThe chemistry of pernitrites. Part I. Kinetics of decomposition of pernitrous acid
Hughes, M., & Nicklin, H. G. (1968) · Journal of the Chemical Society A Inorganic Physical Theoretical · doi:10.1039/j19680000450 · Cited by 356
Metabolic Fate of Peroxynitrite in Aqueous Solution
Pfeiffer, S., Gorren, A. C. F., Schmidt, K., Werner, E. R., Hansert, B., Bohle, D. S., & Mayer, B. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.6.3465 · Cited by 329
Cited by
Sorted by citations
Items 101–143 of 143
A color-tailored fluorogenic sensor for hydrogen peroxide
Potekhina, E. S., Bass, D. I., Ezeriņa, D., Fleckenstein, D. D., Chebotarev, A. S., Сысоева, В. А., Maltsev, D. I., Pak, V. V., Moshchenko, A. A., Соколов, А. И., et al. (2025) · Nature Chemical Biology · doi:10.1038/s41589-025-02036-6 · Cited by 11
Ferreyra, M. G., Fina, B. L., Milardovich, N. J., Chamorro, J. C., Santamaría, B., Balestrasse, K., & Prevosto, L. (2022) · Plasma · doi:10.3390/plasma5020021 · Cited by 11
Campolo, N., Mastrogiovanni, M., Mariotti, M., Issoglio, F. M., Estrin, D. A., Hägglund, P., Grune, T., Davies, M. J., Bartesaghi, S., & Radí, R. (2023) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2023.102941 · Cited by 11
Wada, M., Kira, M., Kido, H., Ikeda, R., Kuroda, N., Nishigaki, T., & Nakashima, K. (2010) · Luminescence · doi:10.1002/bio.1206 · Cited by 11
Ma, Q., Ren, J., Huang, H., Wang, S., Wang, X., & Fan, Z. (2012) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2012.02.033 · Cited by 11
Liang, Y., & Yu, C. (2011) · Drug Testing and Analysis · doi:10.1002/dta.282 · Cited by 11
Cyclic Hydroxylamines as Monitors of Peroxynitrite and Superoxide-Revisited
Samuni, U., Samuni, A., & Goldstein, S. (2021) · Antioxidants · doi:10.3390/antiox11010040 · Cited by 10
Self-citationOxidative Release of Copper from Pharmacologic Copper Bis(thiosemicarbazonato) Compounds
Sirois, J. J., Padgitt‐Cobb, L. K., Gallegos, M. A., Beckman, J. S., Beaudry, C. M., & Hurst, J. K. (2018) · Inorganic Chemistry · doi:10.1021/acs.inorgchem.8b00853 · Cited by 10
Liang, Y., Song, J., & Tian, T. (2004) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-004-2841-5 · Cited by 10
Yang, X. (2008) · Analytica Chimica Acta · doi:10.1016/j.aca.2008.04.032 · Cited by 9
Şentürk, A., Alver, A., Karkucak, M., Küçük, M., & Rendi, T. A. (2023) · The American Journal of the Medical Sciences · doi:10.1016/j.amjms.2023.09.002 · Cited by 8
Arylnitroalkenes as scavengers of macrophage-generated oxidants
Celano, L., Carabio, C., Frache, R., Cataldo, N., Cerecetto, H., González, M., & Thomson, L. (2014) · European Journal of Medicinal Chemistry · doi:10.1016/j.ejmech.2013.12.029 · Cited by 9
Stenman, D., Carlsson, M., & Reitberger, T. (2004) · Journal of Wood Chemistry and Technology · doi:10.1081/wct-200026553 · Cited by 8
Zorzi, V., Berardinelli, A., Gozzi, G., Ragni, L., Vannini, L., Ceccato, R., & Parrino, F. (2023) · Chemosphere · doi:10.1016/j.chemosphere.2023.137792 · Cited by 6
Tomasina, F., Martínez, J., Zeida, A., Chiribao, M. L., Demicheli, V., Correa, A., Quijano, C., Castro, L., Carnahan, R. H., Vinson, P. N., et al. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2213432119 · Cited by 5
Chemiluminescence characterisation of the reaction system Tb(III)–amino acid–peroxynitrous acid
Kaczmarek, M., & Lis, S. (2007) · Journal of Alloys and Compounds · doi:10.1016/j.jallcom.2007.04.173 · Cited by 5
Oxidation of Diethyl Sulfide in Aqueous Solutions by Peroxynitrite and the H2O2-NO−2 System
Lobachev, V. L., Zimtseva, G. P., & Rudakov, E. S. (2005) · Theoretical and Experimental Chemistry · doi:10.1007/s11237-005-0056-2 · Cited by 4
Nitric oxide delivery system for biological media
Skinn, B. T., Lim, C. H., & Deen, W. M. (2010) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2010.10.713 · Cited by 4
Celedón, G., González, G., Lissi, E. A., Cerda, T., Bascuñant, D., Lepeley, M., Pazos, F., Lanio, M. E., & Álvarez, C. (2010) · Free Radical Research · doi:10.3109/10715762.2010.536838 · Cited by 4
Liu, Q., Liu, F., Fan, W., Luo, C., Wang, F., & Liu, X. (2021) · Ionics · doi:10.1007/s11581-020-03887-w · Cited by 4
Hoinkis, N., & Litter, M. I. (2022) · Industrial & Engineering Chemistry Research · doi:10.1021/acs.iecr.2c02296 · Cited by 4
Feibel, D., Kwiatkowski, A., Opländer, C., Grieb, G., Windolf, J., & Suschek, C. V. (2023) · Biomedicines · doi:10.3390/biomedicines11020244 · Cited by 4
Carlsson, M., Stenman, D., Merényi, G., & Reitberger, T. (2005) · Holzforschung · doi:10.1515/hf.2005.020 · Cited by 4
Zhang, N., Gao, X., Zhang, W., Higazy, D., Wang, K., Fu, Z., & Cui, M. (2019) · Molecular Biology Reports · doi:10.1007/s11033-019-05211-w · Cited by 3
Meat Freshness: Peroxynitrite’s Oxidative Role, Its Natural Scavengers, and New Measuring Tools
Vasilescu, A., Vezeanu, A., Liu, Y., Hosu, I. S., Worden, R. M., & Peteu, S. F. (2014) · ACS symposium series · doi:10.1021/bk-2014-1185.ch016 · Cited by 3
Qian, Y., Chen, X., Qi, J., & Liu, X. (2018) · RSC Advances · doi:10.1039/c8ra07087c · Cited by 3
Vayssié, S., & Elias, H. (1999) · Inorganic Chemistry · doi:10.1021/ic981423t · Cited by 3
Carlsson, M. (2005) · KTH Publication Database DiVA (KTH Royal Institute of Technology) · Cited by 2
Barium oxides immobilized SO4- and NO2 as chemiluminescence reaction media.
Hajime, G. O. T. O., Lin, J., & Yamada, M. (2000) · BUNSEKI KAGAKU · doi:10.2116/bunsekikagaku.49.547 · Cited by 1
Tret’yakov, V. P., Min’ko, L. A., & Rudakov, E. S. (2004) · Theoretical and Experimental Chemistry · doi:10.1023/b:thec.0000049077.65698.7c · Cited by 1
Electron transfer. 153. Internal electron transfer to bound cobalt(III) induced by hydroxyl radical
Babich, O. A., & Gould, E. S. (2003) · Research on Chemical Intermediates · doi:10.1163/156856703765694291 · Cited by 1
Interactions of Non-Thermal Air Plasmas with Aqueous Solutions
Anderson, C. E. (2016) · eScholarship (California Digital Library) · Cited by 1
Chakraborty, S. (2010) · ScholarWorks @ The University of New Orleans (The University of New Orleans)
dos Santos, C. X. D. C. (2018) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/t.46.2018.tde-18092018-154812
Mechanistic investigations of the reactions of peroxynitrite with hemoglobin and myoglobin
Exner, M. (2003) · Repository for Publications and Research Data (ETH Zurich) · doi:10.3929/ethz-a-004741527
Buettner, G. R., Oberley, L. W., Spitz, D. R., & Domann, F. E. (2003)
The role of dietary phenolic compounds in the detoxification of reactive nitrogen species
MORTON, L. W. (2003) · UWA Profiles and Research Repository (University of Western Australia)
Husaini, R. (2014) · Bradford Scholars (University of Bradford)
Ultra-Weak Chemiluminescence from Decomposition of Peroxynitrous/Peroxynitrite
Chen, H., & Lin, J. (2022) · Springer eBooks · doi:10.1007/978-3-662-64841-4_3
(2004) · Elsevier eBooks · doi:10.1016/b978-044451721-0/50016-5
Human Mn-superoxide dismutase acetylation protects from enzyme nitration and inactivation
Frattini, G., Puzzolo, J. L., Drusin, S. I., Mansilla, S., Radí, R., Demicheli, V., & Moreno, D. M. (2025) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2025.110439
Wu, L., Liu, S., & Liu, H. (2025) · Environmental Science Water Research & Technology · doi:10.1039/d5ew00997a
Metadata
- Authors
- Saha, A., Goldstein, S., Cabelli, D., & Czapski, G.
- Affiliations
- Hebrew University of Jerusalem (IL)
- Brookhaven National Laboratory (US)
- Corresponding author
- Czapski, Gidon
- Venue
- Free Radical Biology and Medicine
- Publisher
- Elsevier BV
- Published
- 1998-03
- Volume
- 24
- Issue
- 4
- Pages
- 653-659
- ISSN
- 0891-5849 (ISSN-L)
- OpenAlex
- W2079636467
- Cited by
- 143
- References
- 33
- Citations by year
- 2025: 4 · 2024: 4 · 2023: 5 · 2022: 6 · 2021: 4 · 2020: 3 · 2019: 3 · 2018: 6 · 2016: 5 · 2015: 1 · 2014: 8 · 2013: 2 · 2012: 4
- FWCI
- 6.34
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Free Radical Biology and Medicine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Organic Chemistry › Physical Organic Chemistry and Reaction Kinetics
- Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- peroxynitrite, peroxynitrous acid, hydrogen peroxide, nitrite, reaction mechanism, activation energy, chloride, nitrosyl chloride, reaction yield optimization, quenching
- MeSH terms
- Cold Temperature, Hydrogen Peroxide, Hydrogen-Ion Concentration, Kinetics, Nitrates, Nitrites, Osmolar Concentration, Perchlorates, Solutions, Thermodynamics, Oxidants
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
