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Tetrahydrobiopterin enhances forearm vascular response to acetylcholine in both normotensive and hypertensive individuals
Abstract
BACKGROUND: A deficiency of tetrahydrobiopterin (BH4), an essential cofactor for nitric oxide (NO) synthase, decreases NO synthesis and increases superoxide production. Supplementation of BH4 has been postulated to improve endothelial function in atherosclerotic patients. The purpose of this study was to determine whether BH4 restores endothelium-dependent vasodilation in patients with essential hypertension. METHODS: We evaluated the effects of BH4 on forearm vascular responses to acetylcholine (ACh), an endothelium-dependent vasodilator, and isosorbide dinitrate (ISDN), an endothelium-independent vasodilator, both in patients with essential hypertension and in age- and sex-matched normal control subjects. Forearm blood flow (FBF) was measured using strain gauge plethysmography. RESULTS: The response of FBF to ACh was less in hypertensive patients (n = 8) than in normal control subjects (n = 8). There was no significant difference in FBF response to ISDN in the two groups. During coinfusion of BH4 (500 mg/min), the FBF response to ACh in hypertensive patients increased significantly (14.8 +/- 4.6 to 25.6 +/- 7.3 mL/min/100 mL tissue, P < .05) to the level of normal control subjects. In the control subjects, also, BH4 augmented the FBF response to ACh (27.8 +/- 8.7 to 36.1 +/- 9.6 mL/min/100 mL tissue, P < .05). The increase in FBF after ISDN was not altered by BH4 in either group (each group, n = 6). CONCLUSION: Supplementation of BH4 augments endothelium-dependent vasodilation in both normotensive and hypertensive individuals.
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“Conversely, low levels of BH4 are found in endothelial dysfunction and cardiovascular disease (421, 429, 440, 550, 560, 858, 899).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [429]
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Metadata
- Authors
- Higashi, Y., Sasaki, S., Nakagawa, K., Fukuda, Y., Matsuura, H., Oshima, T., & Chayama, K.
- Affiliations
- Hiroshima University (JP)
- Corresponding author
- Higashi, Y.
- Venue
- American Journal of Hypertension
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-04-01
- Volume
- 15
- Issue
- 4
- Pages
- 326-332
- ISSN
- 0895-7061 (ISSN-L)
- 1941-7225
- OpenAlex
- W2028671428
- Cited by
- 173
- References
- 31
- Citations by year
- 2025: 5 · 2024: 4 · 2023: 6 · 2022: 4 · 2021: 5 · 2020: 3 · 2019: 3 · 2018: 5 · 2017: 6 · 2016: 6 · 2015: 9 · 2014: 9 · 2013: 11 · 2012: 18
- FWCI
- 2.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Hypertension (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Health and Disease Prevention
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Blood Pressure and Hypertension Studies
- Keywords
- tetrahydrobiopterin, BH4 supplementation, endothelium-dependent vasodilation, acetylcholine, forearm blood flow, essential hypertension, isosorbide dinitrate, nitric oxide synthase cofactor, endothelial function, forearm circulation
- MeSH terms
- Acetylcholine, Adult, Biopterins, Drug Synergism, Endothelium, Vascular, Female, Forearm, Humans, Hypertension, Male, Middle Aged, Nitric Oxide, Regional Blood Flow, Nitric Oxide Synthase, Nitric Oxide Synthase Type III
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