Actin-Dependent Regulation of Neurotransmitter Release at Central Synapses
Open accessAbstract
Depolymerization of actin by latrunculin A transiently promotes neurotransmitter release. The mean rate of mEPSCs increases by a Ca2+-independent process, without a concomitant change in the mean amplitude. The readily releasable vesicle pool size and the rate of refilling of the readily releasable pool remain unaltered by latrunculin treatment. Evoked neurotransmitter release also increases in a manner consistent with an increase in vesicle release probability. The observed enhancement of neurotransmitter release is specific to actin depolymerization mediated by latrunculin A and is not caused by cytochalasin D. Our findings indicate that actin participates in a regulatory mechanism that restrains fusion of synaptic vesicles at the active zone.
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Overexpression of GFP-β-actin does not impair neuronal function or dendritic spine morphology and density.
“Overexpression of GFP-β-actin does not impair neuronal function or dendritic spine morphology and density and can, therefore, be used to highlight dendritic spines for imaging and quantification [29], [45].”
From Arf4 Determines Dentate Gyrus-Mediated Pattern Separation by Regulating Dendritic Spine Development (2012) · cites this paper as [45]
The Results (page unavailable) show that GFP–β-actin was expressed in hippocampal neurons and visualized puncta on their dendrites, supporting its use as an imaging marker. They do not establish that overexpression leaves neuronal function or dendritic spine morphology and density unimpaired; the reported electrophysiology and morphology comparisons concern latrunculin A treatment, not GFP–β-actin overexpression. (LLM, conf=0.79)
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Cites
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Arf4 Determines Dentate Gyrus-Mediated Pattern Separation by Regulating Dendritic Spine Development
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The WAVE complex in developmental and adulthood brain disorders
Kim, H.-G., Berdasco, C., Nairn, A. C., & Kim, Y. (2025) · Experimental & Molecular Medicine · doi:10.1038/s12276-024-01386-w · Cited by 8 · see citing works
- Authors
- Morales, M., Colicos, M. A., & Goda, Y.
- Affiliations
- University of California San Diego (US)
- Corresponding author
- Goda, Yukiko
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2000-09
- Volume
- 27
- Issue
- 3
- Pages
- 539-550
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2016979915
- Cited by
- 300
- References
- 5
- Citations by year
- 2026: 3 · 2025: 13 · 2024: 1 · 2023: 5 · 2022: 10 · 2021: 9 · 2020: 6 · 2019: 9 · 2018: 8 · 2017: 11 · 2016: 16 · 2015: 14 · 2014: 9 · 2013: 14 · 2012: 17
- FWCI
- 5.54
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- actin, neurotransmitter release, latrunculin A, actin depolymerization, miniature EPSCs, readily releasable pool, synaptic vesicle fusion, release probability, active zone, central synapses, cytochalasin D
- MeSH terms
- Actins, Animals, Calcium, Cells, Cultured, Egtazic Acid, Fluorescent Antibody Technique, Hippocampus, Luminescent Proteins, Marine Toxins, Nerve Tissue Proteins, Synaptic Transmission, Peptides, Cyclic, Recombinant Fusion Proteins, Synapses, Synaptic Vesicles, Thiazoles, Cytochalasin D, Presynaptic Terminals, Neurotransmitter Agents, Bridged Bicyclo Compounds, Heterocyclic, Excitatory Postsynaptic Potentials, Depsipeptides, Green Fluorescent Proteins, Mice, Rats, Thiazolidines
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