The organization of cytoplasm at the presynaptic active zone of a central nervous system synapse

Landis, Dennis M.D.; Hall, Alison K.; Weinstein, Lori A.; Reese, Thomas S.

doi:10.1016/0896-6273(88)90140-7

Cited by 335Neuroscience and Neuropharmacology ResearchCellular transport and secretionExtracellular vesicles in disease

The organization of cytoplasm at the presynaptic active zone of a central nervous system synapse

Landis, Dennis M.D., Hall, Alison K., Weinstein, Lori A., Reese, Thomas S.·1988·Neuron

DOI 10.1016/0896-6273(88)90140-7

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Abstract

The axoplasm at the presynaptic active zone of excitatory synapses between parallel fibers and Purkinje cell spines contains a meshwork of distinct filaments intermingled with synaptic vesicles, seen most clearly after the rapid freezing, freeze-etch technique of tissue preparation. One set of filaments extends radially from synaptic vesicles and intersects similar filaments associated with vesicles as well as larger filaments arising from the presynaptic membrane. The small, vesicle-associated filaments appear to link synaptic vesicles to one another and to enmesh them in the vicinity of the synaptic junction. The vesicle-associated filaments could be synapsin I because they have the same molecular dimensions and are distributed in the same pattern as synapsin I immunoreactivity.

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In cryo-EM studies of unfixed and unstained synapse preparations, no dense projections are detectable; the only structures visible are the plasma membrane, synaptic vesicles, and sparse filaments that connect vesicles to each other or tether vesicles to the presynaptic plasma membrane.
“In cryo-EM studies of unfixed and unstained synapse preparations, however, no dense projections are detectable. The only structures visible are the plasma membrane, synaptic vesicles, and sparse filaments that either connect vesicles to each others (“connectors,” average length ~10 nm) or tether vesicles to the presynaptic plasma membrane (“tethers”—5–20 nm; Landis et al., 1988; Fernández-Busnadie”
From The Presynaptic Active Zone (2012) · cites this paper as Landis et al., 1988

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Cytoplasmic Organization
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Self-citation

Authors: Landis, D. M. D., Hall, A. K., Weinstein, L. A., & Reese, T. S.
Affiliations: Case Western Reserve University (US)
National Institutes of Health (US)
ORCID: Hall, Alison K.
Reese, Thomas S.
Venue: Neuron
Publisher: Elsevier BV
Published: 1988-05
Volume: 1
Issue: 3
Pages: 201-209
ISSN: 0896-6273 (ISSN-L)
DOI: 10.1016/0896-6273(88)90140-7
OpenAlex: W2024032585
Cited by: 333
References: 46
Citations by year: 2026: 2 · 2025: 1 · 2024: 4 · 2023: 6 · 2022: 4 · 2021: 6 · 2020: 3 · 2019: 8 · 2018: 10 · 2017: 9 · 2016: 4 · 2015: 7 · 2014: 12 · 2013: 10 · 2012: 12
FWCI: 5.23
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Neuron (publishedVersion)
PubMed (publishedVersion)
Funders: National Institute of Neurological Disorders and Stroke (NS 23641)
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords: active zone, axoplasm, synaptic vesicles, parallel fibers, synapsin I, cytoskeletal filaments, synapses, excitatory synapses, central nervous system synapses
MeSH terms: Animals, Cerebellum, Cytoplasm, Mice, Inbred C57BL, Microscopy, Electron, Nerve Tissue Proteins, Synapses, Synapsins, Mice

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The organization of cytoplasm at the presynaptic active zone of a central nervous system synapse

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