Mutations in a C. elegans Gqα Gene Disrupt Movement, Egg Laying, and Viability
Open accessAbstract
We find that C. elegans egl-30 encodes a heterotrimeric G protein a subunit more than 80% identical to mammalian Gqalpha family proteins, and which can function as a Gqalpha subunit in COS-7 cells. We have identified new egl-30 alleles in a selection for genes involved in the C. elegans acetylcholine response. Two egl-30 alleles specify premature termination of Gqalpha and are essentially lethal in homozygotes. Animals homozygous for six other egl-30 alleles are viable and fertile, but exhibit delayed egg laying and leave flattened tracks. Overexpression of the wild-type egl-30 gene produces the opposite behavior. Analysis of these mutants suggest that their phenotypes reflect defects in the muscle or neuromuscular junction.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
egl-30 loss-of-function mutants have a straight posture and are almost immobile on food.
“In C. elegans, loss-of-function mutants in the trimeric G-protein Gαq (encoded by egl-30) have a straight posture and are almost immobile on food”
From Two Rab2 Interactors Regulate Dense-Core Vesicle Maturation (2014) · cites this paper as Brundage et al., 1996
The movement results (p. 5) report that egl-30 mutants moved less, and that ad805 and n715 animals were almost paralyzed when undisturbed on an E. coli lawn. But the other strains were described as sluggish rather than almost immobile. The paper reports shallow or flattened movement tracks and flaccidity, not a straight posture for all loss-of-function mutants. (LLM, conf=0.84)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
THE GENETICS OF CAENORHABDITIS ELEGANS
Brenner, S. (1974) · Genetics · doi:10.1093/genetics/77.1.71 · Cited by 14,985 · see citing works
Diversity of G Proteins in Signal Transduction
Simon, M. I., Strathmann, M., & Gautam, N. (1991) · Science · doi:10.1126/science.1902986 · Cited by 2,016 · see citing works
Self-citationThe 2.0 Å crystal structure of a heterotrimeric G protein
Lambright, D. G., Sondek, J., Bohm, A., Skiba, N. P., Hamm, H. E., & Sigler, P. B. (1996) · Nature · doi:10.1038/379311a0 · Cited by 1,136 · see citing works
Baynash, A. G., Hosoda, K., Giaid, A., Richardson, J., Emoto, N., Hammer, R., & Yanagisawa, M. (1994) · Cell · doi:10.1016/0092-8674(94)90018-3 · Cited by 974 · see citing works
The 2.2 Å crystal structure of transducin-α complexed with GTPγS
Noel, J. P., Hamm, H. E., & Sigler, P. B. (1993) · Nature · doi:10.1038/366654a0 · Cited by 803 · see citing works
EGG-LAYING DEFECTIVE MUTANTS OF THE NEMATODE CAENORHABDITIS ELEGANS
Trent, C., Tsung, N., & Horvitz, H. R. (1983) · Genetics · doi:10.1093/genetics/104.4.619 · Cited by 729 · see citing works
Isozyme-selective stimulation of phospholipase C-β2 by G protein βγ-subunits
Camps, M., Carozzi, A., Schnabel, P., Scheer, A., Parker, P. J., & Gierschik, P. (1992) · Nature · doi:10.1038/360684a0 · Cited by 629 · see citing works
Toward a physical map of the genome of the nematode Caenorhabditis elegans
Coulson, A. R., Sulston, J., Brenner, S., & Karn, J. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.20.7821 · Cited by 621 · see citing works
Koelle, M. R., & Horvitz, H. R. (1996) · Cell · doi:10.1016/s0092-8674(00)80998-8 · Cited by 573 · see citing works
Kinesin-related gene unc-104 is required for axonal transport of synaptic vesicles in C. elegans
Hall, D. H., & Hedgecock, E. M. (1991) · Cell · doi:10.1016/0092-8674(91)90391-b · Cited by 564 · see citing works
Regulation and cell autonomy during postembryonic development of Caenorhabditis elegans
Sulston, J. E., & White, J. G. (1980) · Developmental Biology · doi:10.1016/0012-1606(80)90353-x · Cited by 560 · see citing works
Subunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase C
Katz, A. A., Wu, D., & Simon, M. I. (1992) · Nature · doi:10.1038/360686a0 · Cited by 514 · see citing works
Self-citationThe genetics of feeding in Caenorhabditis elegans.
Avery, L. (1993) · Genetics · doi:10.1093/genetics/133.4.897 · Cited by 500 · see citing works
Self-citationG protein diversity: a distinct class of alpha subunits is present in vertebrates and invertebrates.
Strathmann, M., & Simon, M. I. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.23.9113 · Cited by 466 · see citing works
Self-citationStructure and function of receptors coupled to G proteins
Baldwin, J. M. (1994) · Current Opinion in Cell Biology · doi:10.1016/0955-0674(94)90134-1 · Cited by 388 · see citing works
G protein-coupled signal transduction pathways for interleukin-8
Wu, D. W., LaRosa, G. J., & Simon, M. I. (1993) · Science · doi:10.1126/science.8316840 · Cited by 383 · see citing works
Self-citationEffects of starvation and neuroactive drugs on feeding in Caenorhabditis elegans
Avery, L., & Horvitz, H. R. (1990) · Journal of Experimental Zoology · doi:10.1002/jez.1402530305 · Cited by 361 · see citing works
Self-citationWilliams, B., & Waterston, R. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.124.4.475 · Cited by 348 · see citing works
Activation and depalmitoylation of Gsα
Wedegaertner, P. (1994) · Cell · doi:10.1016/0092-8674(94)90445-6 · Cited by 344 · see citing works
Genes required for GABA function in Caenorhabditis elegans
Mclntire, S. L., Jørgensen, E. M., & Horvitz, H. R. (1993) · Nature · doi:10.1038/364334a0 · Cited by 314 · see citing works
Modulation of Serotonin-Controlled Behaviors by G o in Caenorhabditis elegans
Ségalat, L., Elkes, D. A., & Kaplan, J. M. (1995) · Science · doi:10.1126/science.7886454 · Cited by 309 · see citing works
Wu, D. W., Katz, A. A., Lee, C., & Simon, M. I. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)35680-1 · Cited by 295 · see citing works
Self-citationA cell that dies during wild-type C. elegans development can function as a neuron in a ced-3 mutant.
Avery, L., & Horvitz, H. (1987) · Cell · doi:10.1016/0092-8674(87)90593-9 · Cited by 295 · see citing works
Self-citationInteracting genes required for pharyngeal excitation by motor neuron MC in Caenorhabditis elegans.
Raizen, D. M., Lee, R., & Avery, L. (1995) · Genetics · doi:10.1093/genetics/141.4.1365 · Cited by 289 · see citing works
Self-citationThe G-Protein Linked Receptor Facts Book
Watson, S. P., & Arkinstall, S. (1994) · Cited by 287 · see citing works
Wilkie, T. M., Scherle, P., Strathmann, M., Slepak, V. Z., & Simon, M. I. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.22.10049 · Cited by 282 · see citing works
Self-citationMembers of the Gq alpha subunit gene family activate phospholipase C beta isozymes.
Lee, C., Park, D., Wu, D. W., Rhee, S. G., & Simon, M. I. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)41962-x · Cited by 281 · see citing works
Self-citationMutants with altered muscle structure in Caenorhabditis elegans
Waterston, R., Thomson, J. N., & Brenner, S. (1980) · Developmental Biology · doi:10.1016/0012-1606(80)90475-3 · Cited by 271 · see citing works
The basal component of the nematode dense-body is vinculin
Barstead, R. J., & Waterston, R. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)81782-3 · Cited by 253 · see citing works
Muscarinic acetylcholine receptor subtypes in smooth muscle
Eglen, R. M., Reddy, H., Watson, N., et al. (1994) · Trends in Pharmacological Sciences · doi:10.1016/0165-6147(94)90047-7 · Cited by 249 · see citing works
Participation of the Protein G o in Multiple Aspects of Behavior in C. elegans
Mendel, J., Korswagen, H. C., Liu, K. S., Hajdu-Cronin, Y. M., Simon, M. I., Plasterk, R. H. A., & Sternberg, P. W. (1995) · Science · doi:10.1126/science.7886455 · Cited by 249 · see citing works
Self-citationReceptor regulation of G-protein palmitoylation.
Mumby, S. M., Kleuss, C., & Gilman, A. G. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.7.2800 · Cited by 231 · see citing works
Genetic and pharmacological analysis of neurotransmitters controlling egg laying in C. elegans
Weinshenker, D., Garriga, G., & Thomas, J. (1995) · Journal of Neuroscience · doi:10.1523/jneurosci.15-10-06975.1995 · Cited by 222 · see citing works
The C. elegans unc-104 4 gene encodes a putative kinesin heavy chain-like protein
Otsuka, A. J., Jeyaprakash, A., Garcı́a-Añoveros, J., Tang, L., Fisk, G. J., Hartshorne, T. A., Franco, R., & Bornt, T. (1991) · Neuron · doi:10.1016/0896-6273(91)90126-k · Cited by 217 · see citing works
Park, E.-C., & Horvitz, H. R. (1986) · Genetics · doi:10.1093/genetics/113.4.821 · Cited by 192 · see citing works
The G-protein linked receptor facts book
Sibley, D. R. (1994) · Trends in Pharmacological Sciences · doi:10.1016/0165-6147(94)90094-9 · Cited by 183 · see citing works
Transfected muscarinic acetylcholine receptors selectively couple to Gi-type G proteins and Gq/11.
Offermanns, S., Wieland, T., Homann, D., et al. (1994) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)10203-4 · Cited by 171 · see citing works
dgq: A drosophila gene encoding a visual system-specific Gα molecule
Lee, Y. J., Dobbs, M., Verardi, M. L., et al. (1990) · Neuron · doi:10.1016/0896-6273(90)90349-k · Cited by 138 · see citing works
Motor Neuron M3 Controls Pharyngeal Muscle Relaxation Timing in Caenorhabditis Elegans
Avery, L. (1993) · Journal of Experimental Biology · doi:10.1242/jeb.175.1.283 · Cited by 135 · see citing works
Self-citationTwo types of sites required for meiotic chromosome pairing in Caenorhabditis elegans.
McKim, K. S., Peters, K., & Rose, A. M. (1993) · Genetics · doi:10.1093/genetics/134.3.749 · Cited by 117 · see citing works
Alfonso, A., Grundahl, K. M., McManus, J., & Rand, J. B. (1994) · Journal of Neuroscience · doi:10.1523/jneurosci.14-04-02290.1994 · Cited by 81 · see citing works
Homologous and unique G protein alpha subunits in the nematode Caenorhabditis elegans.
Lochrie, M. A., Mendel, J., Sternberg, P. W., et al. (1991) · Cell Regulation · doi:10.1091/mbc.2.2.135 · Cited by 79 · see citing works
Self-citationSplicing in Caenorhabditis elegans does not require an AG at the 3' splice acceptor site.
Aroian, R. V., Levy, A. D., Koga, M., et al. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.1.626 · Cited by 71 · see citing works
Characterization of a G-protein β-subunit gene from the nematode Caenorhabditis elegans
van der Voorn, L., Gebbink, M. F. B. G., Plasterk, R. H. A., et al. (1990) · Journal of Molecular Biology · doi:10.1016/s0022-2836(05)80118-4 · Cited by 64 · see citing works
Functional Analysis of a Dominant Negative Mutant of Gαi2
Slepak, V. Z., Katz, A. A., & Simon, M. I. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.8.4037 · Cited by 56 · see citing works
Self-citationAnalysis of dominant mutations affecting muscle excitation in Caenorhabditis elegans.
Reiner, D. J., Weinshenker, D., & Thomas, J. H. (1995) · Genetics · doi:10.1093/genetics/141.3.961 · Cited by 54 · see citing works
Gollasch, M., Haller, H. G., Schultz, G., et al. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.22.10262 · Cited by 46 · see citing works
Edgerton, M. D., Chabert, C., Chollet, A., et al. (1994) · FEBS Letters · doi:10.1016/0014-5793(94)01101-x · Cited by 42 · see citing works
Gene Structure of MurineGna11andGna15:Tandemly Duplicated Gq Class G Protein α Subunit Genes
Davignon, I., Barnard, M., Gavrilova, O., et al. (1996) · Genomics · doi:10.1006/geno.1996.0059 · Cited by 31 · see citing works
Culotti, J., & Klein, W. (1983) · Journal of Neuroscience · doi:10.1523/jneurosci.03-02-00359.1983 · Cited by 25 · see citing works
Offermanns, S., Gollasch, M., Hescheler, J., et al. (1991) · Molecular Endocrinology · doi:10.1210/mend-5-7-995 · Cited by 22 · see citing works
Characterization of a G-protein α-subunit gene from the nematode Caenorhabditis elegans
Silva, I. F., & Plasterk, R. H. A. (1990) · Journal of Molecular Biology · doi:10.1016/s0022-2836(05)80160-3 · Cited by 20 · see citing works
Splicing in Caenorhabditis elegans Does Not Require an AG at the 3' Splice Acceptor Site
Aroian, R. V., Levy, A. D., Koga, M., et al. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.1.626-637.1993 · Cited by 19 · see citing works
Flagged first, then by citations
Research works printed but not linked: 4
- Edgerton MD, Chabert C, Chollet A, Arkinstall S. Palmitoylation but not the extreme aminoterminusof G q α is required for coupling to the NK2 receptor. FEBS Lett. 1994; 354:195-199. [PubMed: 7957923]
- Lee CH, Park D, Wu D, Rhee SG, Simon MI. Members of the G q α subunit gene family activate phospholipase C β isozymes. J Biol Chem. 1992; 267:16044-16047. [PubMed: 1322889]
- Sulston, J.; Hodgkin, J. Methods. In: Wood, WB., editor. The Nematode Caenorhabditis elegans. Cold Spring Harbor, New York: Cold Spring Harbor Press; 1988. p. 587-606.
- Watson, S.; Arkinstall, S. The G-Protein Linked Receptor FactsBook. New York: Academic Press; 1994.
Cited by
Sorted by citations
Neurobiology of the <i>Caenorhabditis elegans</i> Genome
Bargmann, C. I. (1998) · Science · doi:10.1126/science.282.5396.2028 · Cited by 929 · see citing works
Fujiwara, M., Sengupta, P., & McIntire, S. L. (2002) · Neuron · doi:10.1016/s0896-6273(02)01093-0 · Cited by 344 · see citing works
Bastiani, C. A., Gharib, S., Simon, M. I., & Sternberg, P. W. (2003) · Genetics · doi:10.1093/genetics/165.4.1805 · Cited by 99 · see citing works
Self-citationTwo Rab2 Interactors Regulate Dense-Core Vesicle Maturation
Ailion, M., Hannemann, M., Dalton, S., Pappas, A., Watanabe, S., Hegermann, J., Liu, Q., Han, H.-F., Gu, M., Goulding, M. Q., et al. (2014) · Neuron · doi:10.1016/j.neuron.2014.02.017 · Cited by 86 · see citing works
Charlie, N. K., Schade, M. A., Thomure, A. M., & Miller, K. G. (2006) · Genetics · doi:10.1534/genetics.105.049577 · Cited by 98 · see citing works
Wang, L., Sato, H., Satoh, Y., Tomioka, M., Kunitomo, H., & Iino, Y. (2017) · The Journal of Neuroscience · doi:10.1523/jneurosci.1774-16.2017 · Cited by 37 · see citing works
Schwarz, J., & Bringmann, H. (2017) · eLife · doi:10.7554/elife.24846 · Cited by 10 · see citing works
- Authors
- Brundage, L., Avery, L., Katz, A., Kim, U.-J., Mendel, J. E., Sternberg, P. W., & Simon, M. I.
- Affiliations
- California Institute of Technology (US)
- The University of Texas Southwestern Medical Center (US)
- Corresponding author
- Simon, Melvin I
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 1996-05
- Volume
- 16
- Issue
- 5
- Pages
- 999-1009
- ISSN
- 0896-6273 (ISSN-L)
- PMC
- PMC4444781
- OpenAlex
- W2009821005
- Cited by
- 195
- References
- 53
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 2 · 2023: 4 · 2022: 5 · 2021: 5 · 2020: 6 · 2019: 1 · 2018: 7 · 2017: 10 · 2016: 5 · 2015: 2 · 2014: 6 · 2013: 13 · 2012: 3
- FWCI
- 2.65
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- CaltechAUTHORS (California Institute of Technology) (submittedVersion)
- Funders
- National Heart, Lung, and Blood Institute (R37HL046154, HL46154, R01HL046154)
- National Institute of Neurological Disorders and Stroke (F32NS09541-03, F32NS009541)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Keywords
- egl-30, Gαq, heterotrimeric G proteins, Caenorhabditis elegans, acetylcholine response, oviposition, locomotion, neuromuscular junction, muscle defects, early termination, gene overexpression, mutant allele, viability
- MeSH terms
- Amino Acid Sequence, Animals, Arecoline, Base Sequence, Female, Molecular Sequence Data, Movement, Muscle Contraction, Mutation, Oviposition, Sequence Alignment, Caenorhabditis elegans, Sequence Homology, Amino Acid, DNA Primers, GTP-Binding Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
