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Cited by 426

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SR Splicing Factors Serve as Adapter Proteins for TAP-Dependent mRNA Export

Huang, Yingqun, Gattoni, Renata, Stévenin, James, Steitz, Joan A.·2003·Molecular Cell
DOI 10.1016/s1097-2765(03)00089-3
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Abstract

The only mammalian RNA binding adapter proteins known to partner with TAP/NXF1, the primary receptor for general mRNA export, are members of the REF family. We demonstrate that at least three shuttling SR (serine/arginine-rich) proteins interact with the same domain of TAP/NXF1 that binds REFs. Included are 9G8 and SRp20, previously shown to promote the export of intronless RNAs. A peptide derived from the N terminus of 9G8 inhibits the binding of both REF and SR proteins to TAP/NXF1 in vitro, and this finding argues for competitive interactions. In Xenopus oocytes, the N terminus of 9G8 exhibits a dominant-negative effect on mRNA export from the nucleus, while addition of excess TAP/NXF1 overcomes this inhibition. Thus, multiple adapters including SR proteins most likely cooperate to recruit multiple copies of TAP/NXF1 for efficient mRNA export.

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Cited by

Metadata

Authors
Huang, Y., Gattoni, R., Stévenin, J., & Steitz, J. A.
Venue
Molecular Cell
Publisher
Elsevier BV
Published
2003-03
Volume
11
Issue
3
Pages
837-843
ISSN
1097-2765
DOI
10.1016/s1097-2765(03)00089-3
Cited by
426
References
1
Language
en
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