The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Effects of Bacillus thuringiensis Cry1Ab and Cry3Aa endotoxins on predatory Coleoptera tested through artificial diet-incorporation bioassays
Abstract
Traditional approaches to studying the effects of genetically modified (GM) crops on beneficial insects involve either field assays, comparing insect population levels between control and GM crops or tritrophic bioassays with contaminated insects – usually larvae or eggs of Lepidoptera – as preys. Here, we report the results of a bioassay using an artificial diet, suitable for predatory Coleoptera, to supply Bacillus thuringiensis(Bt) solubilized Cry1Ab and Cry3Aa as well as trypsin-activated Cry1Ab to Atheta coriaria and Cryptolaemus montrouzieri adults and young larvae of Adalia bipunctata. Water, solubilization buffer and trypsin-treated solubilization buffer were used as controls. In total, 1600 insects were assayed. Assays showed a relatively low mortality rate in the controls, ranging from as low as 7% after 15 days (C. montrouzieri) to about 15–20% after five days (A. bipunctata) or 15 days (A. coriaria). For all three predators, there were no statistical differences between the mortality recorded in any of the treatment groups and the corresponding controls. These results indicate a lack of short- (A. bipunctata) and long-term (A. coriaria and C. montrouzieri) mortality associated with oral ingestion of Cry1Ab and Cry3Aa at the high dose tested (50 μg ml−1). We discuss the relevance of these findings for the ecology of beneficial Coleoptera and compatibility with Bt and GM Bt crops.
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Studies with Dalotia coriaria indicated no effects on different life table parameters when beetles were fed spider mites reared on Cry1Ab- or Cry3Bb-producing maize or when purified Cry3Aa and Cry1Ab was provided in artificial diet.
“Studies with Dalotia coriaria (Col.: Staphylinidae) indicated no effects on different life table parameters when beetles were fed spider mites reared on Cry1Ab-[53] or Cry3Bb-producing maize [54] or when purified Cry3Aa and Cry1Ab was provided in artificial diet [55].”
From Does the growing of Bt maize change abundance or ecological function of non-target animals compared to the growing of non-GM maize? A systematic review (2022) · cites this paper as [55]
Except for Staphylinidae, no controlled exposure studies examining direct toxic effects of Bt proteins are available for taxa where our meta-analyses showed lower populations in Bt maize.
“With the exception of Staphylinidae [53–55], no controlled exposure studies to examine direct toxic effects of Bt proteins are available for the taxa where our meta-analyses showed lower populations in Bt maize.”
From Does the growing of Bt maize change abundance or ecological function of non-target animals compared to the growing of non-GM maize? A systematic review (2022) · cites this paper as [55]
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Metadata
- Authors
- Porcar, M., García-Robles, I., Domínguez-Escribà, L., & Latorre, A.
- Affiliations
- Universitat de València (ES)
- Corresponding author
- Porcar, M.
- Venue
- Bulletin of Entomological Research
- Publisher
- Cambridge University Press (CUP)
- Published
- 2009-09-28
- Volume
- 100
- Issue
- 3
- Pages
- 297-302
- ISSN
- 0007-4853 (ISSN-L)
- 1475-2670
- OpenAlex
- W2085550508
- Cited by
- 37
- References
- 22
- Citations by year
- 2025: 1 · 2022: 6 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 1 · 2014: 5 · 2013: 3 · 2012: 8
- FWCI
- 1.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bulletin of Entomological Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
- Keywords
- Bacillus thuringiensis, Cry1Ab, Cryptolaemus montrouzieri, Adalia bipunctata, oral toxicity, genetically modified crops, beneficial insects, non-target effects, insect mortality
- MeSH terms
- Bacillus thuringiensis Toxins, Animals, Bacillus thuringiensis, Bacterial Proteins, Coleoptera, Biological Assay, Diet, Endotoxins, Hemolysin Proteins, Larva, Pest Control, Biological, Predatory Behavior, Survival Analysis
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