Assessment of prey-mediated effects of the coleopteran-specific toxin Cry3Bb1 on the generalist predator Atheta coriaria (Coleoptera: Staphylinidae)
Abstract
A laboratory study was carried out to assess the potential prey-mediated effects of Cry3Bb1-expressing Bt maize on the fitness and predatory ability of Atheta coriaria Kraatz (Coleoptera: Staphylinidae), using Tetranychus urticae Koch (Acari: Tetranychidae) as prey. The concentration of Cry3Bb1 toxin through the trophic chain significantly decreased from Bt maize (21.7 μg g−1 FW) to mites (5.6 μg g−1 FW) and then to A. coriaria adults (1.4 μg g−1 FW), but not from mites to A. coriaria L 1–L 3 larvae (4.1–4.6 μg g−1 FW). Interestingly, the toxin levels detected in A. coriaria larvae represent more than 20% of the concentration found in Bt maize, and the toxin was detected up to 48 h after exposure. To our knowledge, this is the highest level of exposure ever reported in a predatory beetle to the Cry3Bb1 protein. When A. coriaria larvae were reared on Bt-fed mites, Bt-free mites or rearing food, no significant differences among treatments were observed in development, morphological measurements of sclerotized structures and body weight. Moreover, no negative effects on reproductive parameters were reported in adults feeding on Bt-fed prey after 30 days of treatment, and survival was not affected after 60 days of exposure. Similarly, predatory ability and prey consumption of A. coriaria larvae and adults were not affected by exposure to the toxin. All together, these results indicate a lack of adverse effects on A. coriaria, a species commonly used as a biological control agent. The use of A. coriaria as a surrogate species for risk assessment of GM crops that express insecticidal proteins is discussed.
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Studies with Dalotia coriaria indicated no effects on different life table parameters when beetles were fed spider mites reared on Cry1Ab- or Cry3Bb-producing maize or when purified Cry3Aa and Cry1Ab was provided in artificial diet.
“Studies with Dalotia coriaria (Col.: Staphylinidae) indicated no effects on different life table parameters when beetles were fed spider mites reared on Cry1Ab-[53] or Cry3Bb-producing maize [54] or when purified Cry3Aa and Cry1Ab was provided in artificial diet [55].”
From Does the growing of Bt maize change abundance or ecological function of non-target animals compared to the growing of non-GM maize? A systematic review (2022) · cites this paper as [54]
Except for Staphylinidae, no controlled exposure studies examining direct toxic effects of Bt proteins are available for taxa where our meta-analyses showed lower populations in Bt maize.
“With the exception of Staphylinidae [53–55], no controlled exposure studies to examine direct toxic effects of Bt proteins are available for the taxa where our meta-analyses showed lower populations in Bt maize.”
From Does the growing of Bt maize change abundance or ecological function of non-target animals compared to the growing of non-GM maize? A systematic review (2022) · cites this paper as [54]
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Meissle, M., Naranjo, S. E., & Romeis, J. (2022) · Environmental Evidence · doi:10.1186/s13750-022-00272-0 · Cited by 41 · see citing works
“: Staphylinidae) indicated no effects on different life table parameters when beetles were fed spider mites reared on Cry1Ab-[53] or Cry3Bb-producing maize [54] or when purified Cry3Aa and Cry1Ab was provided in artificial diet [55].”
BackgroundGuo, Y.-Y., Tian, J.-C., Shi, W.-P., Dong, X.-H., Romeis, J., Naranjo, S. E., Hellmich, R. L., & Shelton, A. M. (2016) · Transgenic Research · doi:10.1007/s11248-015-9917-1 · Cited by 14 · see citing works
- Authors
- García, M., Ortego, F., Castañera, P., & Farinós, G. P.
- Affiliations
- Consejo Superior de Investigaciones Científicas (ES)
- Corresponding author
- Farinós, G.P.
- Venue
- Bulletin of Entomological Research
- Publisher
- Cambridge University Press (CUP)
- Published
- 2011-11-24
- Volume
- 102
- Issue
- 3
- Pages
- 293-302
- ISSN
- 0007-4853 (ISSN-L)
- 1475-2670
- OpenAlex
- W2034876369
- Cited by
- 22
- References
- 40
- Citations by year
- 2023: 1 · 2022: 7 · 2020: 1 · 2017: 2 · 2016: 4 · 2015: 2 · 2014: 2 · 2012: 3
- FWCI
- 0.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bulletin of Entomological Research (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Funders
- Ministerio para la Transición Ecológica y el Reto Demográfico
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Keywords
- Bt maize, Staphylinidae, Tetranychus urticae, trophic transfer, biological control agents, insecticidal proteins, larval development, reproductive parameters
- MeSH terms
- Animals, Coleoptera, Zea mays, Endotoxins, Female, Larva, Male, Predatory Behavior, Reproduction, Food Chain, Plants, Genetically Modified, Tetranychidae
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