Cellular correlates of proneural and Notch-delta gene expression in the regenerating zebrafish retina
Abstract
Fish can regenerate retinal neurons following ocular injury. Evidence is mounting that astrocytic glia function as inducible, regenerative stem cells in this process, but the underlying molecular events that enable neuronal regeneration are comparatively unclear. In the current study gene array, quantitative real-time PCR,in situ hybridization, and immunohistochemical approaches were used to identify, in the damaged retina of adult zebrafish, correlations between transcriptional events and entry into the cell cycle by Müller cells, a type of astrocytic cell present in all vertebrate retinas that is a candidate ‘stem cell’ of regenerated neurons. A proneural gene (achaete-scute homolog 1a,ash1a) and neurogenic components of the Notch signaling pathway, including notch3 and deltaA, were implicated. An injury-induced, enhanced expression of ash1a was observed in Müller cells, which is hypothesized to contribute to the transition of these cells, or their cellular progeny, into a notch3-expressing, regenerative progenitor. A model of vertebrate retinal repair is suggested in which damage-induced expression of proneural genes, plus canonical Notch-Delta signaling, could contribute to retinal stem cell promotion and subsequent regenerative neurogenesis.
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Notch signaling components are induced in the injured adult zebrafish retina.
“Notch signaling components are also induced in the injured adult zebrafish retina”
From HB-EGF is Necessary and Sufficient for Müller Glia Dedifferentiation and Retina Regeneration (2012) · cites this paper as Yurco and Cameron, 2007
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“Notch signaling components are also induced in the injured adult zebrafish retina (Raymond et al., 2006; Yurco and Cameron, 2007).”
BackgroundThe Proneural Basic Helix-Loop-Helix Gene <i>Ascl1a</i> Is Required for Retina Regeneration
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- Authors
- YURCO, P., & CAMERON, D. A.
- Affiliations
- SUNY Upstate Medical University (US)
- Venue
- Visual Neuroscience
- Publisher
- Maximum Academic Press
- Published
- 2007-05
- Volume
- 24
- Issue
- 3
- Pages
- 437-443
- ISSN
- 0952-5238 (ISSN-L)
- 1469-8714
- OpenAlex
- W1976928467
- Cited by
- 35
- References
- 44
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 1 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 2 · 2013: 2 · 2012: 2
- FWCI
- 1.32
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Visual Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- retinal regeneration, zebrafish retina, Müller cells, astrocytes, retinal stem cells, proneural genes, Notch signaling, Notch3, cell cycle re-entry, ocular trauma
- MeSH terms
- Animals, Eye Injuries, Eye Proteins, Gene Expression Regulation, Nerve Regeneration, Nerve Tissue Proteins, Retina, Stem Cells, Transcription Factors, Zebrafish, In Situ Hybridization, Homeodomain Proteins, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Zebrafish Proteins, Basic Helix-Loop-Helix Proteins, Receptors, Notch, Receptor, Notch1
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