Cited by 35Retinal Development and DisordersNeurogenesis and neuroplasticity mechanismsDevelopmental Biology and Gene Regulation

Cellular correlates of proneural and Notch-delta gene expression in the regenerating zebrafish retina

YURCO, PATRICK, CAMERON, DAVID A.·2007·Visual Neuroscience

Abstract

Fish can regenerate retinal neurons following ocular injury. Evidence is mounting that astrocytic glia function as inducible, regenerative stem cells in this process, but the underlying molecular events that enable neuronal regeneration are comparatively unclear. In the current study gene array, quantitative real-time PCR,in situ hybridization, and immunohistochemical approaches were used to identify, in the damaged retina of adult zebrafish, correlations between transcriptional events and entry into the cell cycle by Müller cells, a type of astrocytic cell present in all vertebrate retinas that is a candidate ‘stem cell’ of regenerated neurons. A proneural gene (achaete-scute homolog 1a,ash1a) and neurogenic components of the Notch signaling pathway, including notch3 and deltaA, were implicated. An injury-induced, enhanced expression of ash1a was observed in Müller cells, which is hypothesized to contribute to the transition of these cells, or their cellular progeny, into a notch3-expressing, regenerative progenitor. A model of vertebrate retinal repair is suggested in which damage-induced expression of proneural genes, plus canonical Notch-Delta signaling, could contribute to retinal stem cell promotion and subsequent regenerative neurogenesis.

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Crossref · 10.1017/s0952523807070496

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Authors
YURCO, P., & CAMERON, D. A.
Affiliations
SUNY Upstate Medical University (US)
Venue
Visual Neuroscience
Published
2007-05
Volume
24
Issue
3
Pages
437-443
ISSN
  • 0952-5238 (ISSN-L)
  • 1469-8714
OpenAlex
W1976928467
Cited by
35
References
44
Citations by year
2025: 2 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 1 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 2 · 2013: 2 · 2012: 2
FWCI
1.32
Type
journal article
Language
en
Open access
closed
Locations
  • Visual Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
retinal regeneration, zebrafish retina, Müller cells, astrocytes, retinal stem cells, proneural genes, Notch signaling, Notch3, cell cycle re-entry, ocular trauma
MeSH terms
Animals, Eye Injuries, Eye Proteins, Gene Expression Regulation, Nerve Regeneration, Nerve Tissue Proteins, Retina, Stem Cells, Transcription Factors, Zebrafish, In Situ Hybridization, Homeodomain Proteins, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Zebrafish Proteins, Basic Helix-Loop-Helix Proteins, Receptors, Notch, Receptor, Notch1

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