Acheate-scute like 1 (Ascl1) is required for normal delta-like (Dll) gene expression and notch signaling during retinal development
Open accessAbstract
Delta gene expression in Drosophila is regulated by proneural basic helix–loop–helix (bHLH) transcription factors, such as acheate‐scute. In vertebrates, multiple Delta‐like and proneural bHLH genes are expressed during neurogenesis, especially in the retina. We recently uncovered a relationship between Acheate‐scute like 1 (Ascl1), Delta‐like genes, and Notch in chick retinal progenitors. Here, we report that mammalian retinal progenitors are also the primary source of Delta‐like genes, likely signaling through Notch among themselves, while differentiating neurons expressed Jagged2. Ascl1 is coexpressed in Delta‐like and Notch active progenitors, and required for normal Delta‐like gene expression and Notch signaling. We also reveal a role for Ascl1 in the regulation of Hes6, a proneurogenic factor that inhibits Notch signaling to promote neural rather than glial differentiation. Thus, these results suggest a molecular mechanism whereby attenuated Notch levels coupled with reduced proneurogenic activity in progenitors leads to increased gliogenesis and decreased neurogenesis in the Ascl1‐deficient retina. Developmental Dynamics 238:2163–2178, 2009. © 2009 Wiley‐Liss, Inc.
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Ascl1 regulates Notch ligand genes during retinal development.
“Ascl1 impacts Notch signaling during retina development by regulating the expression of genes encoding Notch ligands”
From HB-EGF is Necessary and Sufficient for Müller Glia Dedifferentiation and Retina Regeneration (2012) · cites this paper as Nelson et al., 2009
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“Ascl1 impacts Notch signaling during retina development by regulating the expression of genes encoding Notch ligands (Nelson et al., 2009; Nelson and Reh, 2008).”
BackgroundInfluentialASCL1 reprograms mouse Müller glia into neurogenic retinal progenitors
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- Authors
- Nelson, B. R., Hartman, B. H., Ray, C. A., Hayashi, T., Bermingham‐McDonogh, O., & Reh, T. A.
- Affiliations
- University of Washington (US)
- Corresponding author
- Nelson, Branden R.
- Reh, Thomas A.
- Venue
- Developmental Dynamics
- Publisher
- Wiley
- Published
- 2009-02-02
- Volume
- 238
- Issue
- 9
- Pages
- 2163-2178
- ISSN
- 1058-8388 (ISSN-L)
- 1097-0177
- PMC
- PMC2905851
- OpenAlex
- W2130411917
- Cited by
- 94
- References
- 94
- Citations by year
- 2025: 8 · 2024: 3 · 2023: 8 · 2022: 8 · 2021: 6 · 2020: 10 · 2019: 3 · 2018: 5 · 2017: 3 · 2016: 4 · 2015: 6 · 2014: 5 · 2013: 10 · 2012: 5
- FWCI
- 2.01
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Developmental Dynamics (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Eye Institute (F32 EY15631, EY13475, R01EY013475, F32EY015631)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (T32HD007183)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- ASCL1, Notch signaling, retinal development, retinal progenitor cells, HES6, Jagged2, gliogenesis, neurogenesis, chick retina
- MeSH terms
- Serrate-Jagged Proteins, Jagged-1 Protein, Jagged-2 Protein, Animals, Calcium-Binding Proteins, Cell Differentiation, Eye, Membrane Proteins, Mice, Inbred C57BL, Nerve Tissue Proteins, Neurons, Repressor Proteins, Retina, Stem Cells, Signal Transduction, Polymerase Chain Reaction, In Situ Hybridization, Mice, Knockout, Gene Expression Regulation, Developmental, Drosophila Proteins, Intercellular Signaling Peptides and Proteins, Intracellular Signaling Peptides and Proteins, Adaptor Proteins, Signal Transducing, Mice, Basic Helix-Loop-Helix Proteins, Receptors, Notch
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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