Cited by 94Retinal Development and DisordersDevelopmental Biology and Gene RegulationNeurogenesis and neuroplasticity mechanisms

Acheate-scute like 1 (Ascl1) is required for normal delta-like (Dll) gene expression and notch signaling during retinal development

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Nelson, Branden R., Hartman, Byron H., Ray, Catherine A., Hayashi, Toshinori, Bermingham‐McDonogh, Olivia, Reh, Thomas A.·2009·Developmental Dynamics

Abstract

Delta gene expression in Drosophila is regulated by proneural basic helix–loop–helix (bHLH) transcription factors, such as acheate‐scute. In vertebrates, multiple Delta‐like and proneural bHLH genes are expressed during neurogenesis, especially in the retina. We recently uncovered a relationship between Acheate‐scute like 1 (Ascl1), Delta‐like genes, and Notch in chick retinal progenitors. Here, we report that mammalian retinal progenitors are also the primary source of Delta‐like genes, likely signaling through Notch among themselves, while differentiating neurons expressed Jagged2. Ascl1 is coexpressed in Delta‐like and Notch active progenitors, and required for normal Delta‐like gene expression and Notch signaling. We also reveal a role for Ascl1 in the regulation of Hes6, a proneurogenic factor that inhibits Notch signaling to promote neural rather than glial differentiation. Thus, these results suggest a molecular mechanism whereby attenuated Notch levels coupled with reduced proneurogenic activity in progenitors leads to increased gliogenesis and decreased neurogenesis in the Ascl1‐deficient retina. Developmental Dynamics 238:2163–2178, 2009. © 2009 Wiley‐Liss, Inc.

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Authors
Nelson, B. R., Hartman, B. H., Ray, C. A., Hayashi, T., Bermingham‐McDonogh, O., & Reh, T. A.
Affiliations
University of Washington (US)
Corresponding author
  • Nelson, Branden R.
  • Reh, Thomas A.
Venue
Developmental Dynamics
Publisher
Wiley
Published
2009-02-02
Volume
238
Issue
9
Pages
2163-2178
ISSN
  • 1058-8388 (ISSN-L)
  • 1097-0177
OpenAlex
W2130411917
Cited by
94
References
94
Citations by year
2025: 8 · 2024: 3 · 2023: 8 · 2022: 8 · 2021: 6 · 2020: 10 · 2019: 3 · 2018: 5 · 2017: 3 · 2016: 4 · 2015: 6 · 2014: 5 · 2013: 10 · 2012: 5
FWCI
2.01
Type
journal article
Language
en
Open access
Locations
  • Developmental Dynamics (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health
  • National Eye Institute (F32 EY15631, EY13475, R01EY013475, F32EY015631)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (T32HD007183)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords
ASCL1, Notch signaling, retinal development, retinal progenitor cells, HES6, Jagged2, gliogenesis, neurogenesis, chick retina
MeSH terms
Serrate-Jagged Proteins, Jagged-1 Protein, Jagged-2 Protein, Animals, Calcium-Binding Proteins, Cell Differentiation, Eye, Membrane Proteins, Mice, Inbred C57BL, Nerve Tissue Proteins, Neurons, Repressor Proteins, Retina, Stem Cells, Signal Transduction, Polymerase Chain Reaction, In Situ Hybridization, Mice, Knockout, Gene Expression Regulation, Developmental, Drosophila Proteins, Intercellular Signaling Peptides and Proteins, Intracellular Signaling Peptides and Proteins, Adaptor Proteins, Signal Transducing, Mice, Basic Helix-Loop-Helix Proteins, Receptors, Notch

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